Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis

Gonçalves, Ricardo Marcelo; Balbi-Peña, María Isabel; Soman, José Marcelo; Maringoni, Antônio Carlos; Taghouti, Géraldine; Saux, Marion Fischer-Le; Portier, Pérrine

doi:10.1007/s10658-018-01648-0

Cited by 17 publications

(11 citation statements)

References 29 publications

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“…The two evolutionary lineages, yellow‐pigmented strains and red‐/orange‐pigmented strains, are distinct. Orange‐ and red‐pigmented strains of Cff were separated from yellow‐pigmented strains using multilocus sequence analysis (MLSA) of five housekeeping genes, atpD , gyrB , ppk , recA , and rpoB (Osdaghi et al ., ; ; Gonçalves et al , ). Whole‐genome sequencing of Australian Cff isolated from mungbean has shown the presence of multiple lineages (Vaghefi et al ., ).…”

Section: Population Structure and Genetic Diversitymentioning

confidence: 99%

“…oortii (Osdaghi et al , ). Recently, Gonçalves et al () analysed a large population of plant‐pathogenic C. flaccumfaciens strains from all over the world and concluded that there is no correlation between the MLSA scheme and the host specificity of the strains. It seems more likely that the evolutionary forces that drive phylogenetic position and bacteriological characteristics, for example colony pigmentation in C. flaccumfaciens , are dominating the plant pathogenicity features and host specification of the strains.…”

Section: Population Structure and Genetic Diversitymentioning

confidence: 99%

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Bacterial wilt of dry beans caused by Curtobacterium flaccumfaciens pv. flaccumfaciens: A new threat from an old enemy

Osdaghi

Young

Harveson

2020

Molecular Plant Pathology

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.In this review, we provide an updated overview on the aetiology, epidemiology, and management of bacterial wilt of dry beans and the current taxonomic status of the causal agent Curtobacterium flaccumfaciens pv. flaccumfaciens. AbstractBacterial wilt and tan spot of dry beans (family Fabaceae), caused by Curtobacterium flaccumfaciens pv. flaccumfaciens, is an important emerging disease threatening the edible legume industry around the globe. The management of bacterial wilt has been a major problem since its original description in 1922. This is in part due to the seedborne nature of the pathogen allowing the bacterium to be transmitted long distances via infected seeds, as well as a lack of detailed molecular information concerning the pathogenicity repertoires and virulence determinates of the pathogen. Identification can also be difficult owing to the presence of five different colony colour variants (i.e., yellow, orange, pink, purple, and red) on culture media. In this review, we provide an overview of the aetiology, epidemiology, and management strategies of bacterial wilt disease. First, a comprehensive and comparative symptomology of the disease on different dry bean species is described. Then, the taxonomic history of the causal agent and utility of high-throughput sequencing-based approaches in the precise characterization of the pathogen is explained. Furthermore, we provide an updated outline on the global distribution of the pathogen, highlighting expansion of the causal agent into the areas with no history of the disease until the beginning of the current century. Finally, because there are limited options for use of conventional pesticides against the pathogen, we highlight the use of integrated pest management strategies, for example quarantine inspections, resistant cultivars, and crop sanitation, to combat the risk of bacterial wilt disease in the dry bean industry. Disease symptoms: Interveinal chlorosis on leaflets leading to necrotic areas and systemic wilt. Seed discolouration to yellow, orange, pink, or purple is seen in whiteseeded cultivars. Host range: Causes bacterial wilt and tan spot disease on edible dry beans in the Fabaceae family, including common bean (Phaseolus vulgaris), cowpea (Vigna unguiculata), mungbean (Vigna radiata), soybean (Glycine max), as well as a number of weed species.

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Section: Population Structure and Genetic Diversitymentioning

confidence: 99%

Section: Population Structure and Genetic Diversitymentioning

confidence: 99%

Bacterial wilt of dry beans caused by Curtobacterium flaccumfaciens pv. flaccumfaciens: A new threat from an old enemy

Osdaghi

Young

Harveson

2020

Molecular Plant Pathology

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“…1). Six housekeeping genes (atpD, dnaK, gyrB, ppK, recA, and rpoB), which had been used to construct a phylogenetic tree of C. accumfaciens were all identi ed in the core genome (Goncalves et al 2019). A total of 4,296 genes were found in more than two, but not all 12 genomes, these constituted the accessory genomes (Fig.…”

Section: Pangenome Of C Accumfaciensmentioning

confidence: 99%

“…However, there is no obvious morphological difference between pathogenic and nonpathogenic C. accumfaciens strains . Showing high genetic diversity amongst the strains, a phylogenetic tree of C. accumfaciens was constructed by multilocus sequence analysis (MLSA) based on six housekeeping genes (Goncalves et al 2019). Nevertheless, to further reveal the differences between the pathogenic and nonpathogenic strains, systems biology approaches such as genomics is necessary.…”

Section: Introductionmentioning

confidence: 99%

Genomic Analysis of Curtobacterium Flaccumfaciens Reveals the Differences Between Pathogenic and Nonpathogenic Strains

Sun

2021

Preprint

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Purpose Curtobacterium flaccumfaciens is a Gram-positive bacterium which has been isolated from different plants and abiotic environment. Curtobacterium. flaccumfaciens pv. flaccumfaciens (Cff) is a pathogenic bacterium that infects legume, which is causing great economic losses. At the genomic level, the metabolic and phylogenetic characteristics, and differences in pathogenicity between pathogenic and nonpathogenic C. flaccumfaciens strains have not been analyzed in detail. Methods Therefore, in order to discuss the differences in genome, phylogeny, gene function and mobile genetic elements between pathogenic and nonpathogenic strains, pangenomics and comparative genomics were used in this study to analyze 12 C. flaccumfaciens strains. Result The pangenome of C. flaccumfaciens is open. Phylogenetic analysis showed that there was no correlation between the phylogeny and pathogenicity of C. flaccumfaciens. KAAS annotation of the core genome shows that the citrate cycle was incomplete. In addition, gene islands analysis of the three pathogenicity-related genes encoding for pectate lyase, serine protease and cellulases showed that they only existed in the Cffs and LMG3645 strains. LMG3645 might be a pathogenic strain. Conclusion This study clearly and reliably revealed the differences between the pathogenic and nonpathogenic strains of C. flaccumfaciens at the genomic level, and paves the way for further research on its pathogenicity.

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“…Genetic typing studies (e.g., repetitive element sequence-based PCR, amplified fragment length polymorphism) of C. flaccumfaciens pv. flaccumfaciens strains have shown a large degree of heterogeneity (Agarkova et al 2012;Gonçalves et al 2019), but genomic resources necessary for further investigation of the genetic diversity among C. flaccumfaciens pv. flaccumfaciens strains are limited.…”

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confidence: 99%

Complete Genome Sequence Resource of a Strain of Curtobacterium flaccumfaciens pv. flaccumfaciens, the Causal Agent of Bacterial Wilt of Common Bean, from Turkey

O'Leary

Gilbertson

2020

Phytopathology®

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Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is the causal agent of bacterial wilt of common bean (Phaseolus vulgaris), a disease that can reduce yields of this economically important crop worldwide. Current genomics resources for this important bacterial pathogen are limited. Therefore, long-read sequencing was used to determine the complete genome sequence of a pathogenic Cff strain isolated from common bean leaves showing irregular necrotic lesions with yellow borders collected in a commercial field in Turkey in 2015.

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Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis

Cited by 17 publications

References 29 publications

Bacterial wilt of dry beans caused by Curtobacterium flaccumfaciens pv. flaccumfaciens: A new threat from an old enemy

Bacterial wilt of dry beans caused by Curtobacterium flaccumfaciens pv. flaccumfaciens: A new threat from an old enemy

Genomic Analysis of Curtobacterium Flaccumfaciens Reveals the Differences Between Pathogenic and Nonpathogenic Strains

Complete Genome Sequence Resource of a Strain of Curtobacterium flaccumfaciens pv. flaccumfaciens, the Causal Agent of Bacterial Wilt of Common Bean, from Turkey

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