Complete Genome Sequence Resource of a Strain of <i>Curtobacterium flaccumfaciens</i> pv. <i>flaccumfaciens</i>, the Causal Agent of Bacterial Wilt of Common Bean, from Turkey

O'Leary, Michael L; Gilbertson, R. L.

doi:10.1094/phyto-04-20-0131-a

Cited by 6 publications

(4 citation statements)

References 29 publications

(21 reference statements)

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“…The genomes of the plasmids with a low GC contain multiple repeats, CTTT, CCTTTT, and similar, with the overweight of the thymine residues. The plasmids pCff1 and pCff113 attract special interest because of virulence proteins reported to be encoded in their genomes [23]. These plasmids have prominent withingenome similarities, with a linear plasmid, pCSL1, occurring in Clavibacter sepedonicus strain ATCC33113 [23].…”

Section: Genomospecies 49 50mentioning

confidence: 99%

“…The plasmids pCff1 and pCff113 attract special interest because of virulence proteins reported to be encoded in their genomes [23]. These plasmids have prominent withingenome similarities, with a linear plasmid, pCSL1, occurring in Clavibacter sepedonicus strain ATCC33113 [23].…”

Section: Genomospecies 49 50mentioning

confidence: 99%

“…and eight Curtobacterium strains isolated by the authors from infected plants (No. [23][24][25][26][27][28][29][30], as well as 15 strains isolated according to the same protocol but which turned out to be representatives of other genera (No. [31][32][33][34][35][36][37][38][39][40][41][42][43][44][45].…”

Section: Curtobacterium Pcr Diagnostics and Genus-specific Primersmentioning

confidence: 99%

“…Another important question concerns the relatedness between the mechanisms of pathogenicity of curtobacteria and their taxonomy and phylogeny. The genomes of Curtobacterium plasmids have been shown to contain possible virulence genes, and the effect of mobile elements and gene exchange could reveal the ways of the emergence of pathogenic strains and pathovars [20][21][22][23]. In this study, Curtobacterium plasmids were studied in the context of the genomics of pathogenicity.…”

Section: Introductionmentioning

confidence: 99%

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Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics

Evseev

Lukianova

Tarakanov

et al. 2022

CIMB

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The genus of Curtobacterium, belonging to the Microbacteriaceae family of the Actinomycetales order, includes economically significant pathogenic bacteria of soybeans and other agricultural crops. Thorough phylogenetic and full-genome analysis using the latest genomic data has demonstrated a complex and contradictory taxonomic picture within the group of organisms classified as the Curtobacterium species. Based on these data, it is possible to delineate about 50 new species and to reclassify a substantial part of the Curtobacterium strains. It is suggested that 53 strains, including most of the Curtobacterium flaccumfaciens pathovars, can compose a monophyletic group classified as C. flaccumfaciens. A genomic analysis using the most recent inventory of bacterial chromosomal and plasmid genomes deposited to GenBank confirmed the possible role of Microbacteriaceae plasmids in pathogenicity and demonstrated the existence of a group of related plasmids carrying virulence factors and possessing a gene distantly related to DNA polymerase found in bacteriophages and archaeal and eukaryotic viruses. A PCR diagnostic assay specific to the genus Curtobacterium was developed and tested. The presented results assist in the understanding of the evolutionary relations within the genus and can lay the foundation for further taxonomic updates.

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Section: Genomospecies 49 50mentioning

confidence: 99%

Section: Genomospecies 49 50mentioning

confidence: 99%

Section: Curtobacterium Pcr Diagnostics and Genus-specific Primersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics

Evseev

Lukianova

Tarakanov

et al. 2022

CIMB

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Curtobacterium allii sp. nov., the actinobacterial pathogen causing onion bulb rot

Khanal

Bhatta

Timilsina

et al. 2022

Antonie van Leeuwenhoek

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A Gram positive, aerobic, and non-spore-forming bacterial strain, 20TX0166 T , was isolated from a diseased onion bulb in Texas, USA. Upon testing its pathogenicity on onion bulb, it produced pathogenic response which makes it rst species of pathogen belonging to the phylum actinobacteria detected in onion. Phylogenetic analysis of the 16S rRNA gene sequence revealed that the strain belonged to the genus Curtobacterium and was most similar to Curtobacterium accumfaciens LMG 3645 T (100%), C. pusillum DSM 20527 T (99.5%), and C. oceanosedimentum ATCC 31317 T (99.5%). The orthologous ANI (orthoANIu), ANI based on blast (ANIb), and dDDH values between the novel strain and the closest relative, C. accumfaciens LMG 3645 T , were 95.7%, 95.4%, and 63.3%, respectively. These values were below the recommended species cut-off threshold of 96% (ANI) and 70% (dDDH), suggesting the strain may be a novel species. The estimated genome size of the novel species was 3.98 Mbp with a G + C content of 70.8%. Physiologic and phenotypic characters of this novel strain were also unique when compared with the closely related species. The major cellular fatty acids of this strain were C 15:0 anteiso and C 17:0 anteiso. Using a polyphasic approach based on phenotypic and genotypic analyses, strain 20TX0166 T represents a novel species of the genus Curtobacterium, and the name Curtobacterium allii sp. nov.

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Curtobacterium flaccumfaciens pv. flaccumfaciens (bacterial wilt of dry beans)

Osdaghi¹

2022

CABI Compendium

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Complete Genome Sequence Resource of a Strain of Curtobacterium flaccumfaciens pv. flaccumfaciens, the Causal Agent of Bacterial Wilt of Common Bean, from Turkey

Cited by 6 publications

References 29 publications

Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics

Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics

Curtobacterium allii sp. nov., the actinobacterial pathogen causing onion bulb rot

Curtobacterium flaccumfaciens pv. flaccumfaciens (bacterial wilt of dry beans)

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