Genetic Diversity of Cultivated Lentil (Lens culinaris Medik.) and Its Relation to the World's Agro-ecological Zones

Khazaei, Hamid; Caron, Carolyn T.; Fedoruk, Michael; Diapari, Marwan; Vandenberg, Albert; Coyne, Clarice J.; McGee, Rebecca J.; Bett, Kirstin E.

doi:10.3389/fpls.2016.01093

Cited by 100 publications

(96 citation statements)

References 43 publications

(58 reference statements)

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“…The average PIC value was 0.25, which is consistent with common bean (Souza et al, 2012;Galeano et al, 2012;Blair et al, 2013) but lower than for faba bean (Vicia faba L.) and lentil (Lens culinaris Medik.) (Kaur et al, 2014;Khazaei et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Mung Bean Germplasm from the USDA Core Collection Using Newly Developed KASP‐based SNP Markers

Islam

Blair

2018

Crop Science

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Mung bean [Vigna radiata (L.) Wilczek] is an important, nutritious legume food crop for tropical and subtropical countries with high value in Asia and worldwide potential. However, the genetic improvement of mung bean is slowed by low polymorphism of previous molecular markers, even microsatellites. The goals of this study were to develop Kompetitive Allele Specific Polymerase Chain Reaction (KASP)‐based single‐nucleotide polymorphism (SNP) markers and use them for characterization of 94 cultivated mung bean genotypes from the USDA originating in 27 countries across 10 regions of the world, all being cultivars rather than wild accessions. We targeted 42 SNPs from previous sequencing and converted them into 20 robust KASP assays. Of these, 18 were polymorphic among the mung bean cultivars, with 38 alleles total and 1.9 alleles per locus average. The polymorphism information content of the newly developed markers ranged from zero for monomorphic markers to 0.375 for the most diverse biallelic polymorphic marker (MBkSNP_39) and averaged 0.250 across all loci. Population structure analysis grouped 90% of the accessions into two subpopulations, with 10% belonging to an admixture group, but did not follow geographic origins of the germplasm, suggesting no clear center of origin and blending of the subpopulations. An analysis of molecular variance revealed 22% of genetic variation among subpopulations and 78% within subpopulations. The first two axes of region‐wide principle coordinate analysis explained 81.26% variation of total variation, indicating the existence of genetic diversity among groups. The SNP markers of this study can be used in molecular breeding of mung bean and are the first to work with KASP detection.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Mung Bean Germplasm from the USDA Core Collection Using Newly Developed KASP‐based SNP Markers

Islam

Blair

2018

Crop Science

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“…Molecular markers have proven to be valuable tools in the evaluation of genetic variation both within and between species (Powell et al, 1996). Molecular biology has contributed to the development of DNA-based markers that can be used for genotype identification, fingerprinting, genetic mapping and diversity assessment (Khazaei et al, 2016). On the other hand, ISSRs are DNA-based markers permit detection of polymorphisms in inter-microsatellite loci ( Karaca and Izbirak, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation within and among the wild populations of Alhagi graecorum using ISSR markers

et al. 2019

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Alhagi graecorum Boiss. (Camel thorn) (Family Fabaceae) is a wild plant which grows in salty soils and dry environment. It is native to Mediterranean and central Asia. Several studies have revealed the use of Alhagi plants in treating a wide spectrum of diseases. In this study, eight ISSR primers were used to assess genetic diversity of five populations of A. graecorum (El-Dakhla Oasis; Botanical garden of botany department, Ain Shams University; Wadi El-Rayan; Qarun Lake and Siwa Oasis) using individual -and bulked samples-based approaches. Twenty five individuals were sampled, amplified separately and amplified as bulked DNA to identify polymorphism among the studied populations. In total, 101 and 97 bands were scored with 83.3% and 78.4% polymorphism, respectively. Average polymorphism information content (PIC) for amplified DNA of individual samples was 0.875 and bulked-samples was recorded 0.69. Analysis of molecular variance (AMOVA) showed that 63% of the total variation was observed among populations while variation within populations accounted for the remaining 37%. The highest genetic similarity index was (0.99) among the studied Alhagi genotypes was recorded between the two genotypes 6 and 7 both individuals were belonging to Ain Shams University population, while among inter populations was (0.853%) between Qarun Lake and Siwa Oasis populations. The UPGMA cluster analysis divided the 25 individuals and bulked samples into two main groups. Each group was further subdivided into many sub-clusters, PCoA was also carried out and in agreement with the UPGMA. The obtained results demonstrate that the ISSR markers were efficient for evaluation of the genetic relationships between intra-and inter-population due to their efficiency in revealing polymorphism.

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“…Few molecular genetics and genomic resources existed for lentil, partly due to its relatively large genome size (Khazaei et al, 2016). Assessment of genetic diversity of different crop species is appropriate precursor for improvement of the crop because it generates baseline data to guide selection of parental lines.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization and relationships among Egyptian lentil cultivars as revealed by biochemical and molecular markers.

Mahfouz

Rayan

2018

Egypt. J. Bot.

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L ENTIL (Lens culinaris Medikus) is an important seed legume crop cultivated worldwide as human food. Little is known about the molecular structure of the Egyptian lentil. Intervarietal variation and cultivar identification are crucial for breeding and gene bank conservation of this plant worldwide. The SDS-PAGE and SRAP-PCR techniques were used to detect some biochemical and molecular markers of six Egyptian lentil genotypes (Sinai 1, Line 9, Giza 1, Giza 4, Giza 51 and Giza 370). The genetic distance between cultivars was also estimated from banding patterns. The results of SDS-PAGE revealed a total of 21 bands with molecular weight ranging from about to 6-102 KD. The maximum number of bands (14) appeared in Giza 4, while the minimum number of bands (10) appeared in Giza 1. Electrophoresis analysis among six lentil cultivars showed 80.95% polymorphism, while DNA variations were explored using ten combinations of SRAP markers. SRAP analysis among six lentil cultivars showed 61.54% polymorphism. The highest similarity index (0.869) was found between Giza 51and Giza 370. The lowest similarity index (0.603) was found between Giza 1 and Giza 4. The markers used in this study created polymorphic bands among the different cultivars that can be utilized as molecular markers for their differentiation. The obtained data indicated that SRAP and SDS-PAGE systems can efficiently identify and differentiate between the selected cultivars. Also these results, enhancing the available knowledge of lentil genetic resources in Egypt, may contribute to their conservation and utilization in breeding programs.

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Genetic Diversity of Cultivated Lentil (Lens culinaris Medik.) and Its Relation to the World's Agro-ecological Zones

Cited by 100 publications

References 43 publications

Molecular Characterization of Mung Bean Germplasm from the USDA Core Collection Using Newly Developed KASP‐based SNP Markers

Molecular Characterization of Mung Bean Germplasm from the USDA Core Collection Using Newly Developed KASP‐based SNP Markers

Genetic variation within and among the wild populations of Alhagi graecorum using ISSR markers

Genetic characterization and relationships among Egyptian lentil cultivars as revealed by biochemical and molecular markers.

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