Genetic diversity of Capsicum chinensis (Solanaceae) accessions based on molecular markers and morphological and agronomic traits

Abstract

ABSTRACT. We estimated the genetic diversity of 49 accessions of the hot pepper species Capsicum chinensis through analyses of 12 physicochemical traits of the fruit, eight multi-categorical variables, and with 32 RAPD primers. Data from the physicochemical traits were submitted to analysis of variance to estimate the genetic parameters, and their means were clustered by the Scott-Knott test. The matrices from the individual and combined distance were estimated by multivariate analyses before applying Tocher's optimization method. All physicochemical traits were examined for genetic variability by analysis of variance. The responses of these traits showed more contribution from genetic than from environmental factors, except the percentage of dry biomass, content of soluble solids and vitamin C level. Total capsaicin had the greatest genetic divergence. Nine clusters were formed from the quantitative data based on the generalized distance of Mahalanobis, using Tocher's method; four were formed from the multi-categorical data using the Cole-Rodgers coefficient, and eight were formed from the molecular data using the Nei and Li coefficient. The accessions were distributed into 14 groups using Tocher's method, and no significant correlation between pungency and origin was detected. Uni-and multivariate analyses permitted the identification of marked genetic diversity and fruit attributes capable of being improved through breeding programs.

Research Article Characterization of a pepper collection (Capsicum frutescens L.) from Brazil.

“…For all methodologies used, accession CNPH 3453 was the most divergent within the germplasm collection evaluated. It produces pale-orange fruits and, based on its morphological characteristics in comparison to the characterization of C. chinense (Finger et al, 2010;Baba et al, 2016) such accession may belong to the C. chinense species and had been mistakenly allocated in the C. frutescens bank. In the three cluster analysis, CNPH 3453 formed a separate group (Figures 1, 2, and 4).…”

Morphological characterization and analysis of genetic variability among pepper accessions

“…Measurements that presented significant differences among them were obtained in all the characters (Table 2), being these grouped by the Scott e Knott test (SCOTT & KNOTT, 1974) at a level of significance of 1% probability. Other studies on the variability and genetic parameters in Capsicum, using morphological markers are described in the literature (BENTO et al, 2007;FINGER et al, 2010;NEITZKE et al, 2010;RÊGO et al, 2011;DOMENICO et al, 2012;SILVA NETO et al, 2014).…”

Impact of climatic factors on genetic diversity of Stipa breviflora populations in Inner Mongolia

“…Genetic differentiation drives genetic diversity (Schaal et al, 1991) and the relationship between genetic differentiation and environmental factors has been a focus of recent studies (Rossetto et al, 2009;Finger et al, 2010;Santos et al, 2010;Martins et al, 2011). Random amplified polymorphic DNA (RAPD) markers can cover the total genome of organisms, including coding and noncoding regions, and reflect differences in the whole genome (Garcia et al, 1998).…”