Genetic diversity of Cahi DRB and DQB genes of caprine MHC class II in Sirohi goat

Abstract

The objective of this study was to assess the genetic diversity of the Sirohi goat for and loci, and to study their association with antibody response induced by the Peste des petits ruminants (PPR) vaccine. A total of 360 Sirohi kids were studied using single stranded confirmation polymorphism (SSCP) followed by polymerase chain reaction sequence-based typing (PCR-SBT) for and diversities. The competitive enzyme-linked immuno-sorbent assay (C-ELISA) was used to evaluate immune response post-PPR vaccination. Study revealed rich diversity of major histocompatibility complex (MHC) region in goat. A total of 18 and 15 alleles were obtained which were new. Alleles DRB*0104 and DQB1*0101 were the most common. The approach of SSCP combined with PCR-SBT reflects cost-effective and most powerful approach to decipher the genetic diversity in complex MHC region. Study revealed variation in and genes in Sirohi flock along with high Wu-Kabat index. A total of 16 of the 89 amino acid residue sites in and 19 of 86 residue sites in had more than three amino acid substitutions. Positive evolutionary selection was evident in Sirohi for MHC region. Nonsignificant association of and genotypes with PPR virus (PPRV) vaccine response revealed complexity of the phenotype and importance of other factors for vaccine response. Rich diversity of and genes reflects the fitness of the population and importance of this locus for future selection programmes.

Analysis of Genetic Variability in the Caprine Class II MHC CLA DRB3.2(Cahi DRB3.2) gene locus of the Sangamneri goat breed of India

“…The results obtained in the present study, therefore, are indicating that a very high degree of polymorphism is present in the Caprine Class II Major Histo compatibility complex-CLA-DRB3.2 gene locus of the indigenous Sangamneri goat breed animals. Earlier studies have also supported the presence of a high degree of genetic polymorphism in the Class II MHC CLA-DRB3.2 locus (Amills et al, 1995, Takada et al, 1998, Zhao et al, 2011, Gowane et al, 2018 This genetic variability is maintained in this locus since it encodes the antigen binding site(ABS) which comes into direct contact with the different antigen(s). The genetic variability results in the amino acid variability that further leads to the protein being able of attaining varying conformations that aid in its interaction with the varying antigenic peptides being presented by different pathogens and eliciting an appropriate immune response in order to counter the infection of the host by the pathogen and conferring the host with an ability to ght against disease and protect it from the varied plethora of antigens present in the environment, thereby protecting the host from the assaults by the pathogens and contributing to disease resistance/susceptibility of the host.…”

“…Singh et al, (2012, characterized the Class II MHC DRB 3 gene in the endagered Jamunapari goat breed. Gowane et al (2018), characterized the genetic diversity of the Cahi DRB and DQB genes of the Caprine MHC class II in Sirohi goat breed of India.…”

Polymorphism of Selected Regions of Ovar-MHC and the Health Status of the Ovine Mammary Gland

“…Similar polymorphism was demonstrated in our own studies (Tables 1 and 2), even though a different and longer fragment (exon 2/intron 2) of this gene was analyzed. Much greater genetic diversity of the Ovar-DRB1 gene (14 alleles-from 148 bp to 238 bp) was reported by Worley et al [33] in sheep of various breeds in Norway, Canada, and Alaska, Geldermann et al [34] in German merino (16 alleles), as well as Gowane et al [70] in Malpura (23 alleles). The OMHC1 gene polymorphism demonstrated in this study (Tables 1 and 2) is also confirmed in papers by other authors.…”

Genetic diversity estimation of Yunnan indigenous goat breeds using microsatellite markers

“…Currently, the abundant genetic diversity of goats has been assessed by using many different types of molecular markers, including microsatellites (Câmara, Nunes, Diniz, Silva, & Araújo, 2017;E et al, 2018), MHC region variants (Gowane, Akram, Misra, Prakash, & Kumar, 2018), mitochondrial DNA (E et al, 2018;Tarekegn et al, 2018), functional genes (Gowane et al, 2018;Pitarch et al, 2018), and genomewide SNPs (Ilie, Kusza, Sauer, & Gavojdian, 2018;Nicoloso et al, 2015;Onzima et al, 2018). Currently, the abundant genetic diversity of goats has been assessed by using many different types of molecular markers, including microsatellites (Câmara, Nunes, Diniz, Silva, & Araújo, 2017;E et al, 2018), MHC region variants (Gowane, Akram, Misra, Prakash, & Kumar, 2018), mitochondrial DNA (E et al, 2018;Tarekegn et al, 2018), functional genes (Gowane et al, 2018;Pitarch et al, 2018), and genomewide SNPs (Ilie, Kusza, Sauer, & Gavojdian, 2018;Nicoloso et al, 2015;Onzima et al, 2018).…”

“…Goats (Capra hircus) are economically important domestic animals worldwide. Currently, the abundant genetic diversity of goats has been assessed by using many different types of molecular markers, including microsatellites (Câmara, Nunes, Diniz, Silva, & Araújo, 2017;E et al, 2018), MHC region variants (Gowane, Akram, Misra, Prakash, & Kumar, 2018), mitochondrial DNA (E et al, 2018;Tarekegn et al, 2018), functional genes (Gowane et al, 2018;Pitarch et al, 2018), and genomewide SNPs (Ilie, Kusza, Sauer, & Gavojdian, 2018;Nicoloso et al, 2015;Onzima et al, 2018). The diversity of several indigenous goat breeds in China was assessed Liu et al, 2014;Wang et al, 2017).…”