Genetic diversity of cacao accessions selected for resistance to witches’ broom based on RAPD markers

Faleiro, Fábio Gelape; Lopes, Uilson Vanderlei; Yamada, Mamoru; Melo, Gilson Roberto Pires; Monteiro, Wilson Reis; Pires, José Luís; Rocha, Jefferson Bomfim; Bahia, Rita C.S; Gomes, Luana Mahé Costa; Araújo, Ioná Santos; Faleiro, A. S. G.

doi:10.12702/1984-7033.v04n01a03

Cited by 9 publications

(8 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sabe-se apenas que muitas delas originaram-se de poucas fontes de resistência (Yamada et al, 2003). Essas seleções devem ter-se originado de cerca de 18 genitores utilizados pela Ceplac, para a produção dos híbridos distribuídos aos agricultores (Faleiro et al, 2004b).…”

Section: Introductionunclassified

Diversidade genética entre acessos de cacau de fazendas e de banco de germoplasma na Bahia

Leal

Santos

et al. 2008

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi avaliar a diversidade genética de acessos de cacau, selecionados previamente como produtivos e resistentes à vassoura-de-bruxa na Bahia, e estudar suas inter-relações com genótipos no banco de germoplasma. Amostras de DNA de folhas dos 120 acessos, coletados em 17 fazendas de sete municípios do Sul da Bahia, foram amplificadas pela técnica de RAPD ("random amplified polymorphic DNA"). Os coeficientes de dissimilaridade genética, calculados pelo método de Jaccard a partir das bandas RAPD, permitiram evidenciar, pela análise de agrupamento, que a maioria das seleções das fazendas (89,2%) agrupou-se com acessos do banco de germoplasma considerados representativos da diversidade de cacau (híbridos, trinitários, Scavinas, amazônicos e cacau-comum). As demais seleções distribuíram-se em outros sete grupos distintos. Há elevada diversidade genética entre as seleções das fazendas, e algumas delas devem ter-se originado de genitores não incluídos nesta análise. Esses materiais apresentam potencial para seleção de clones com maior diversidade para novos cruzamentos ou uso pelos agricultores.Termos para indexação: Theobroma cacao, marcador molecular, melhoramento genético, vassoura-de-bruxa. Genetic diversity among farm and germplasm bank accessions of cacao in Bahia, BrazilAbstract -The objective of this work was to assess genetic diversity of farm cacao accessions, previously selected as productive and resistant to witch broom in Bahia, Brazil, and to study their interrelationship with reference genotypes on germplasm bank. DNA samples from 120 cacao accessions, collected on 17 farms in seven counties in Southern Bahia, were amplified by RAPD technique (random amplified polymorphic DNA). The coefficients of genetic dissimilarity, calculated from the RAPD bands by Jaccard method, allowed to show by cluster analysis that most farm selections (89.2%) grouped with accessions from germplasm bank considered representatives of the cacao genetic diversity (hybrids, Trinitarian, Scavina, Amazon and common cacao). The other selections were distributed in seven distinct groups. There is high genetic diversity among farm selections, and some of them may have originated from other progenitors not included in this analysis. These materials present potential for clone selection with higher diversity for new crossings or direct use by agriculturists.

show abstract

Section: Introductionunclassified

Diversidade genética entre acessos de cacau de fazendas e de banco de germoplasma na Bahia

Leal

Santos

et al. 2008

Pesq. agropec. bras.

View full text Add to dashboard Cite

show abstract

“…Bragantia, Campinas, v.64, n.3, p. [361][362][363][364][365][366][367][368]2005 The level of GD found in the TSA-644 descendants can be considered low when compared to previous studies in large sets of cocoa germplasm (FALEIRO et al, 2004a) and farmers' selections in southern Bahia (YAMADA, 2001;2004c;LEAL et al, 2004). However, the data revealed that Scavina-6 X IMC 67 descendants maintain a certain level of diversity.…”

Section: Discussionmentioning

confidence: 99%

“…Measurements of genetic variability among and within cocoa populations have been derived from phenotypic and molecular data (BEKELE and BEKELE, 1996;MARITA et al, 2000;FALEIRO et al, 2004a). Phenotypic data has been instrumental in the classification of the major germplasm groups in cocoa, but it is less useful for the separation of accessions within groups (CHARTERS and WILKINSON, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Molecular markers like Random Amplified Polymorphic DNA (RAPD) and microsatellites are currently the most widely used markers for assessing genetic diversity in cocoa (MARITA et al, 2001;FALEIRO et al, 2002b;FALEIRO et al, 2004a;2004b). MARITA et al (2001) using RAPD studied the genetic diversity of a representative sample composed by 280 accessions, providing an overall portrait of the relatedness of the major cocoa germplasm groups available in the world.…”

Section: Introductionmentioning

confidence: 99%

“…MARITA et al (2001) using RAPD studied the genetic diversity of a representative sample composed by 280 accessions, providing an overall portrait of the relatedness of the major cocoa germplasm groups available in the world. RAPD has also been used in genetic diversity studies in clones selected for WB resistance (FALEIRO et al, 2004a), in the identification of mislabeling and accession duplication (FALEIRO et al, 2002c), and in the development of genetic linkage maps (FALEIRO et al 2004b;QUEIRÓZ et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of genetic diversity on a sample of cocoa accessions resistant to witches' broom disease based on RAPD and pedigree data

et al. 2005

View full text Add to dashboard Cite

Genetic diversity in cocoa (Theobroma cacao L.) has been assessed based on morphological and molecular markers for germplasm management and breeding purposes. Pedigree data is available in cocoa but it has not been used for assessing genetic relatedness. The geneitic diversity of 30 clonal cocoa accessions resistant to witche´ broom disease, from the CEPEC series, were studied on the basis of RAPD data and pedigree information. Twenty of these accessions descend from the TSA-644 clone, originated from a cross between the Upper Amazon germplasm called Scavina-6, the main source of resistance to witches' broom disease, and IMC-67. The ten remaining clones come from different sources including Amazon and Trinitario germplasm. RAPD data was collected using 16 primers and pedigree information was obtained from the International Cocoa Germplasm Database. Genetic similarities, genetic distances and coefficient of parentage were calculated using available software. Relatively low genetic diversity was observed in this germplasm set, probably because of great genetic relatedness amongst accessions studied and the poor representation of the germplasm. The TSA-644 descendants were more diverse than the other accessions used in the study. This might be due to the origin of the TSA clone, which was derived from highly divergent genotypes. Association between genetic similarities based on RAPD data and coefficient of parentage, based on pedigree data, was very low, probably due to the homogeneity of the breeding stocks and poor pedigree information. These findings are useful to cocoa breeders in planning crosses for the development of hybrid and clonal cultivars.

show abstract

Genetic Improvement in Cocoa

Monteiro

Lopes²,

Clément³

Breeding Plantation Tree Crops: Tropical Species

View full text Add to dashboard Cite

Genetic diversity of cacao accessions selected for resistance to witches’ broom based on RAPD markers

Abstract: -To increase the genetic diversity sources of resistance to witches

Cited by 9 publications

References 6 publications

Diversidade genética entre acessos de cacau de fazendas e de banco de germoplasma na Bahia

Diversidade genética entre acessos de cacau de fazendas e de banco de germoplasma na Bahia

Assessment of genetic diversity on a sample of cocoa accessions resistant to witches' broom disease based on RAPD and pedigree data

Genetic Improvement in Cocoa

Contact Info

Product

Resources

About