Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil

Mendes, Natália Serra; Ramos, Inalda Angélica de Souza; Herrera, Heitor Miraglia; Campos, João Bosco Vilela; Alves, João Victor de Almeida; Macedo, Gabriel Carvalho de; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.1007/s00436-019-06337-3

Cited by 12 publications

(20 citation statements)

References 50 publications

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“…Hematophagous dipterans, such as horn flies (Haematobia irritans) and tabanids (Tabanus spp.) were also observed (Souza Ramos et al, 2019a, b;Mendes et al, 2019;De Mello et al, 2019).…”

Section: Animals and Study Areamentioning

confidence: 79%

Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

Ramos

Mello

Mendes

et al. 2020

Rev. Bras. Parasitol. Vet.

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This study investigated the seropositivity for five different tick-borne agents, namely Anaplasma marginale, Babesia bovis, Babesia bigemina, Coxiella burnetii, Anaplasma phagocytophilum, and Trypanosoma vivax in beef cattle in the Brazilian Pantanal. The serum samples collected from animals (200 cows; 200 calves) were used in indirect enzyme-linked immunosorbent assays (iELISA) to detect IgG antibodies against A. marginale, B. bovis, B. bigemina, and T. vivax, and Indirect Fluorescent Antibody Test (IFAT) for detecting IgG antibodies against C. burnetii and A. phagocytophilum. No correlation was observed between seropositivity for C. burnetii and A. phagocytophilum with other agents whereas moderate correlation was observed for A. marginalexB. bigemina x B. bovis. Cows were more seropositive for T. vivax whereas calves were more seropositive for B. bovis and B. bigemina. The highest number of seropositive animals by a single agent was observed for T. vivax (15.2%). Co-seropositivity for T. vivax + A. marginale was higher in cows (25.5%) and for T. vivax + B. bovis + B. bigemina + A. marginale was higher in calves (57.5%). The high seropositivity correlation for A. marginale x B. bovis x B. bigemina is probably due to the presence of the tick biological vector, Rhipicephalus microplus, in the studied farms. Common transmission pathways, mediated by hematophagous dipterans and fomites, may explain the high co-seropositivity of cows for A. marginale and T. vivax. Low seropositivity to C. burnetii is probably due to the type of breeding system employed (extensive). Seropositivity for A. phagocytophilum in only one animal suggests the occurrence of a cross-serological reaction with another agent of the genus Anaplasma.

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“…Hematophagous dipterans, such as horn flies (Haematobia irritans) and tabanids (Tabanus spp.) were also observed (Souza Ramos et al, 2019a, b;Mendes et al, 2019;De Mello et al, 2019).…”

Section: Animals and Study Areamentioning

confidence: 79%

Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

Ramos

Mello

Mendes

et al. 2020

Rev. Bras. Parasitol. Vet.

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“…Antigenic polymorphism has been applied as a strategy by most protozoan parasites to escape the immune response of mammalian hosts, and it is a major obstacle preventing the development of effective vaccines against these parasites [7]. In our present study, analysis of the genetic relationships of B. bovis MSA-1, MSA-2b and MSA-2c of Chinese isolates, with respect to almost all available related sequences deposited in the GenBank database (including Mongolia, Vietnam, Philippines, Thailand, Israel, Vietnam, Sri Lanka, Mexico, Argentina, Brazil, Japan and USA) was conducted [7,12,[18][19][20][21][22][23][24]26,30,31]. Phylogenetic analysis showed that the MSA-1 amino acid sequences had a wide distribution in four clades.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China

et al. 2020

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The apicomplexan parasite Babesia bovis is a tick-borne intracellular hemoprotozoan parasite that is widespread across China. Genetic diversity is an important strategy used by parasites to escape the immune responses of their hosts. In our present study, 575 blood samples, collected from cattle in 10 provinces, were initially screened using a nested PCR (polymerase chain reaction) for detection of B. bovis infection. To perform genetic diversity analyses, positive samples were further amplified to obtain sequences of three B. bovis merozoite surface antigen genes (MSA-1, MSA-2b, MSA-2c). The results of the nested PCR approach showed that an average of 8.9% (51/575) of cattle were positive for B. bovis infection. Phylogenetic analyses of the predicted amino acid sequences revealed that unique antigen variants were formed only by Chinese isolates. Our findings provide vital information for understanding the genetic diversity of B. bovis in China.

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“…Among those families mentioned above, the rap1 , msa1 , sbp2 , and 6-cys multigene families and their encoded proteins fall into the variable category. The diversity present in variable genes coupled with their relative stability make them good markers for use in epidemiologic studies at the isolate level, and advantage has been taken of rap1 and msa1 sequences repeatedly for this purpose (for example, see [21,22,23,24,25,26,27,28,29,30]). In contrast, members of the ves multigene family are highly variant in sequence and the expressed member(s) further vary rapidly during the course of infection in an individual host [14].…”

Section: Major Variable and Variant Multigene Protein Familiesmentioning

confidence: 99%

Variable and Variant Protein Multigene Families in Babesia bovis Persistence

Allred

2019

Pathogens

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Cattle infected with Babesia bovis face a bifurcated fate: Either die of the severe acute infection, or survive and carry for many years a highly persistent but generally asymptomatic infection. In this review, the author describes known and potential contributions of three variable or highly variant multigene-encoded families of proteins to persistence in the bovine host, and the mechanisms by which variability arises among these families. Ramifications arising from this variability are discussed.

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Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil

Cited by 12 publications

References 50 publications

Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China

Variable and Variant Protein Multigene Families in Babesia bovis Persistence

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