Genetic diversity of an ectomycorrhizal fungus Tricholoma terreum in a Larix principis-rupprechtii stand assessed using random amplified polymorphic DNA

Huai, Wenxia; Guo, Liang‐Dong; He, Wei

doi:10.1007/s00572-003-0227-8

Cited by 18 publications

(13 citation statements)

References 8 publications

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“…Total DNA was extracted from these samples utilizing a modified CTAB protocol (Huai, Guo, & He, 2003). Total DNA was extracted from these samples utilizing a modified CTAB protocol (Huai, Guo, & He, 2003).…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Fusarium species in declining wild apple forests on the northern slope of the Tian Shan Mountains in north‐western China

et al. 2019

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Wild apple forests in the Tian Shan Mountains in north‐western China have been adversely affected by an unknown disease in recent years. Symptoms attributed to this disease that affects wild apple trees include xylem browning and dieback which are suggestive of infection by Fusarium species. Therefore, the research team conducted the first survey for Fusarium in the afflicted wild apple forests. Twig samples with symptoms of xylem browning and dieback were collected in the Xinyuan, Gongliu, Yining and Huocheng Counties of Xinjiang Uighur Autonomous Region in China. Based on phylogenetic analyses and morphological observation, sixty strains of Fusarium accounted for 48% of the total number of fungi isolated from samples were subsequently classified into six species including twenty‐four F. avenaceum, seventeen F. solani, ten F. tricinctum, five F. proliferatum, two F. sporotrichioides and two unfamiliar Fusarium sp. 1. The five previously known species of Fusarium were then tested for pathogenicity to leaves and twigs in vitro. The results indicated that all of the species, except for F. tricinctum, can cause obvious lesions on the leaves of host plants and on the twigs of Fuji and wild apple. This is the first report of Fusarium species pathogenicity in Xinjiang wild apple forests, confirming a new host for these pathogens in this study.

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Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Fusarium species in declining wild apple forests on the northern slope of the Tian Shan Mountains in north‐western China

et al. 2019

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“…Harvested mycelium was ground using a pestle for 3-8 min, and genomic DNA was extracted using the modified CTAB protocol (Huai et al 2003). Harvested mycelium was ground using a pestle for 3-8 min, and genomic DNA was extracted using the modified CTAB protocol (Huai et al 2003).…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

“…From our preliminary pathogenicity tests using detached leaves as described in De Dobbelaere et al (2010), of four plant species, Ilex purpurea Hassk, Quercus variabilis Blume, Magnolia denudata Desr. Future research on the temporal and spatial distributions of populations of these eight Phytophthora species, along with study of origin and maintenance of genetic variation may promote an understanding of how populations of these coexisting organisms interact and evolve in the succession of forest ecosystems (Huai et al 2003). In fact, the baiting method might exploite the selective pathogenicity of a Phytophthora species to infect and cause lesions on living host tissue.…”

Section: Distribution Of Phytophthora Speciesmentioning

confidence: 99%

Identification of Phytophthora species baited and isolated from forest soil and streams in northwestern Yunnan province, China

et al. 2013

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Phytophthora species were surveyed by collecting soil samples and placing bait leaves in selected streams during June-October in the years 2005, 2006 and 2010 at three sites in oak forests in Diqing Tibetan Autonomous Prefecture of NW Yunnan province, China. Seventy-three isolates of Phytophthora spp. were recovered from 135 baited leaf samples and 81 soil samples. Eight Phytophthora species were identified by observation of morphological features and ITS1-5.8S-ITS2 rDNA sequence analysis. The eight taxa included two well-known species P. gonapodyides and P. cryptogea, two recently described species P. gregata and P. plurivora, two named but as yet undescribed taxa, P. taxon PgChlamydo and P. taxon Salixsoil, and two previously unrecognized species, Phytophthora sp.1 and P. sp.2. The most numerous species, P. taxon PgChlamydo, and the second most abundant species, P. taxon Salixsoil, were recovered at all three sites. Phytophthora cryptogea was detected only once at site Nixi. Phytophthora gregata and P. sp.2 were isolated from a stream only at site Bitahai, while the other three species were each found at two sites. Phylogenetic analysis revealed that the isolates belonged to three ITS clades, one species including six isolates in clade 2, six species including 66 isolates in clade 6 and one species in clade 8. There was a relatively rich species and genetic diversity of Phytophthora detected in the investigated regions where the forest biotic and abiotic factors affecting the growth and evolution of Phytophthora populations were diverse.

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“…Sexual reproduction and recombination were found to be prevalent, and genetic differentiation was significant among those geographical populations. However, little genetic difference was found between samples from pine-dominated forests and alpine, oak/shrub, mixed forests [296][297][298][299][300].…”

Section: Genetic Structure Of Em Speciesmentioning

confidence: 99%

“…Among 33 sporophores from distinct genotypes, genet size was no larger than 0.5 m. The Shannon-Weaver diversity index was 4.52 for these 33 sporophores, and was similar between the phenetic group 1 (4.28, eight sporophores) and group 2 (4.46, 25 sporophores). Sexual spore propagation would appear to be important in the life history of T. terreum in this larch forest [300]. The genomic library of Tricholoma matsutake was constructed from assays of single nucleotide polymorphisms (SNPs) of two strains, 350 km from each other, in southwest China [301].…”

Section: Genetic Structure Of Em Speciesmentioning

confidence: 99%

A 60-year journey of mycorrhizal research in China: Past, present and future directions

Duan

Chen

et al. 2010

Sci. China Life Sci.

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The significance of mycorrhizas (fungal roots in 90% of land plants) in plant nutrient acquisition and growth, element biogeochemical cycling and maintaining of terrestrial ecosystem structures has been globally established for more than 120 years. Great progress in mycorrhizal research in the past 60 years (1950-2009, 1981-2009 in particular) has also been made across China, particularly in the mainland, Hong Kong and Taiwan. For instance, a total of 20 new and approximately 120 records of arbuscular mycorrhizal (AM) fungal species, 30 new and approximately 800 records of ectomycorrhizal (EM) fungal species, a dozen of new and approximately 100 records of orchid mycorrhizal (OM) fungal species have been isolated by morphological observation and/or molecular identification in China since the 1950s. Great accomplishment has also been made in the following area, including fungal species richness and genetic structure, relationships between species composition and plant taxa, effects of mycorrhizal fungi on plant nutrient uptake and growth, resistances to pathogens and interactions with other soil microorganisms, potential of mycorrhizal fungi in phytoremediation and/or land reclamation, alterations of enzymatic activities in mycorrhizal plants, and elevated CO(2) and O(3) on root colonization and species diversity. Unfortunately, the international community cannot easily appreciate almost all Chinese mycorrhizal studies since the vast majority of them have been published in Chinese and/or in China-based journals. The aim of this review is to make a comprehensive exposure of the past and present China's major mycorrhizal research to the whole world, and then to suggest potential directions for the enhancement of future mycorrhizal research within and/or between the Chinese and international mycorrhizal community.

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Genetic diversity of an ectomycorrhizal fungus Tricholoma terreum in a Larix principis-rupprechtii stand assessed using random amplified polymorphic DNA

Cited by 18 publications

References 8 publications

Fusarium species in declining wild apple forests on the northern slope of the Tian Shan Mountains in north‐western China

Fusarium species in declining wild apple forests on the northern slope of the Tian Shan Mountains in north‐western China

Identification of Phytophthora species baited and isolated from forest soil and streams in northwestern Yunnan province, China

A 60-year journey of mycorrhizal research in China: Past, present and future directions

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