Genetic diversity in the non-structural gene of parvovirus B19 detected by single-stranded conformational polymorphism assay (SSCP) and partial nucleotide sequencing

Kerr, Jonathan; Curran, Martin D.; Moore, John E.; Erdman, Dean D.; Coyle, Peter; Nunoue, Tadasu; Middleton, Derek; Ferguson, Wendy

doi:10.1016/0166-0934(95)00017-o

Cited by 33 publications

(20 citation statements)

References 36 publications

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“…Compared to the standard strain, no insertions or deletions among the isolates were identified. The nucleotide and amino acid diversity of the NS1-VP1u coding region between each strain in our study and strain Au was close to 2%, as previously published (13).…”

Section: Serum Anti-b19v Igm and Igg Antibodysupporting

confidence: 67%

“…In the present study, the isolates of subtype A1 from 1981 to 1982 and of subtype A2 from 1986 to 1987 were also different. Kerr et al (13) divided B19V isolates into six types (1a, 1b, and 2 to 5) by a single-stranded conformational polymorphism assay and partial nucleotide sequencing. They collected 50 samples from various countries (United Kingdom, United States, and Japan).…”

Section: Discussionmentioning

confidence: 99%

“…B19V strains are now subdivided into three distinct genotypes: 1, 2, and 3 (11,20,21,29). Genotype 1 is thought to be the most common worldwide.Almost all reports about polygenetic analyses of B19V sequences have shown that genetic diversity is correlated with the respective places and years of isolation (7,8,10,13). However, these studies were based on unsystematic data with an uncoordinated distribution of places and years because sampling occurred at random.…”

mentioning

confidence: 99%

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Analysis of Nucleotide Sequences of Human Parvovirus B19 Genome Reveals Two Different Modes of Evolution, a Gradual Alteration and a Sudden Replacement: a Retrospective Study in Sapporo, Japan, from 1980 to 2008

Suzuki

Yoto

Ishikawa

et al. 2009

J Virol

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There have been no long-term systematic analyses of the molecular epidemiology of human parvovirus B19 (B19V). We investigated the variations of nucleotide sequences of B19V strains collected in Sapporo, Japan, from 1980 to 2008. In that period, six outbreaks of erythema infectiosum occurred regularly at 5-year intervals. The B19V strains collected successively, regardless of the outbreak, were analyzed for nucleotide variation in the subgenomic NS1-VP1u junction. The isolated strains can be classified into 10 subgroups. Two patterns of change of endemic strains were observed. One was a dynamic replacement of strains that occurred almost every 10 years, and the other was a gradual change consisting of an accumulation of point mutations.Human parvovirus B19 (B19V), a small, nonenveloped, single-stranded DNA virus, belongs to the genus Erythrovirus and the family Parvoviridae and is the only erythrovirus known to be pathogenic in humans. B19V usually transmits via a respiratory route and can also be infectious in blood products or blood components (4). B19V DNA replicates restrictively within erythrocyte precursors in bone marrow (23). The most common presentation of B19V infection is erythema infectiosum (fifth disease), which is generally benign and self-limiting (1). B19V causes aplastic crisis in patients with congenital hemolytic anemia (25). Many reports suggest an association between B19V infection and various clinical manifestations, such as hydrops fetalis, arthritis, hepatitis, and meningoencephalitis (2,5,26,33).The function of NS1, which is composed of 671 amino acids (aa), is related to the transactivation of protein for viral DNA replication, and NS1 is known to have cytotoxic properties (9,18). Another open reading frame encodes both VP1 (781 aa) and VP2 (554 aa) for capsids. VP1 and VP2 are identical except for the additional 227-aa N terminus called the VP1 unique region (VP1u), which is exposed to the surface of infected cells and the target sites of neutralizing antibodies (17,27).Genetic diversity among B19V strains has been considered to be low, and nucleotide variation is less than 1 to 2% for the whole genome (7, 10). However, recent reports suggest that the genetic diversity of B19V is greater than previously believed. B19V strains are now subdivided into three distinct genotypes: 1, 2, and 3 (11,20,21,29). Genotype 1 is thought to be the most common worldwide.Almost all reports about polygenetic analyses of B19V sequences have shown that genetic diversity is correlated with the respective places and years of isolation (7,8,10,13). However, these studies were based on unsystematic data with an uncoordinated distribution of places and years because sampling occurred at random. We have screened serum samples collected over the last 3 decades for B19V DNA using a nested PCR assay and analyzed these sequences to determine the epidemiological distribution and transmission patterns of B19V. Our study tracks the genetic evolution of the virus more precisely because it is based on serum samples col...

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Section: Serum Anti-b19v Igm and Igg Antibodysupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Analysis of Nucleotide Sequences of Human Parvovirus B19 Genome Reveals Two Different Modes of Evolution, a Gradual Alteration and a Sudden Replacement: a Retrospective Study in Sapporo, Japan, from 1980 to 2008

Suzuki

Yoto

Ishikawa

et al. 2009

J Virol

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“…B19 strains have been divided into genotypic groups on the basis of restriction site polymorphisms [15][16][17][18] and the single-stranded conformational polymorphism assay [19]. Such assays form part of the routine molecular phylogenetic analysis of viral nucleotide sequences [20].…”

Section: Virologymentioning

confidence: 99%

Neurological manifestations of human parvovirus B19 infection

Barah

Vallely

Cleator

et al. 2003

Reviews in Medical Virology

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Since its discovery, human parvovirus B19 has been linked with a broad spectrum of clinical syndromes. An aetiological role for the virus has been confirmed in erythema infectiosum, transient aplastic crisis, persistent infection manifesting as pure red cell aplasia in immunocompromised persons, non-immune hydrops fetalis and arthritis. Less commonly recognised, but receiving increasing attention recently, are the neurological manifestations, a variety of which have been described in patients with either clinically diagnosed or laboratory confirmed B19 infection. The purpose of this review is to summarise present knowledge of B19, its known and potential pathogenic mechanisms and its association with human diseases, particularly those with neurological manifestations. The outcome of the review supports an aetiological role of the virus in neurological disease. However, the pathogenesis remains unknown and elucidating this is a priority.

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“…A study comparing nine isolates collected over 20 years analysed the regions encoding the VP1-specific and VP1-VP2 junction portions of the capsid and showed that there has been minimal change in the genome (Gallinella et al, 1995). A study of the NS region by Kerr et al (1995) also found very little variability. Originally it was thought there was only one B19 sub-group but now the situation is unclear.…”

Section: Fhgmentioning

confidence: 99%

Persistence of parvovirus B19 DNA in testis of patients with testicular germ cell tumours.

Gray

Guillou

Zufferey

et al. 1998

Journal of General Virology

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Germ cell tumours (GCT) of the testis are the most common malignant tumours occurring in young adults. In view of the young age of patients, the increasing incidence of GCT and the overexpression of wild-type p53 observed in a majority of tumours, the possibility of the involvement of a virus in the development of this cancer was considered. Testicular GCT were analysed for the presence of cytomegalovirus and Epstein-Barr virus (EBV), which are known to cause overexpression of wildtype p53 protein, and parvovirus B19. The testicular tissue of 39 patients with testicular GCT and 12 patients with healthy testicular tissues was tested for presence of viral DNA by PCR. Neither cytomegalovirus nor EBV DNAs were detected in the 39 tumours analysed, but parvovirus B19 DNA

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Genetic diversity in the non-structural gene of parvovirus B19 detected by single-stranded conformational polymorphism assay (SSCP) and partial nucleotide sequencing

Cited by 33 publications

References 36 publications

Analysis of Nucleotide Sequences of Human Parvovirus B19 Genome Reveals Two Different Modes of Evolution, a Gradual Alteration and a Sudden Replacement: a Retrospective Study in Sapporo, Japan, from 1980 to 2008

Analysis of Nucleotide Sequences of Human Parvovirus B19 Genome Reveals Two Different Modes of Evolution, a Gradual Alteration and a Sudden Replacement: a Retrospective Study in Sapporo, Japan, from 1980 to 2008

Neurological manifestations of human parvovirus B19 infection

Persistence of parvovirus B19 DNA in testis of patients with testicular germ cell tumours.

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