Genetic diversity in natural populations of two geographic isolates of Korean black raspberry

Hong, Yi-Huan; Kim, M.-J.; Hong, Kyung-Nak

doi:10.1080/14620316.2003.11511630

Cited by 15 publications

(7 citation statements)

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“…Considering all genotypes the average polymorphic fragments per primer was of 11.3 with an average polymorphism of 78.72% (Table 4). These results are concordant with some results in similar studies in Rosaceae using ISSR markers 36 , especially in Rubus. In this regard, Sedighi and Rahimmalek (2015) 6 used ISSR primer to assess the genetic diversity in several populations of R. hyrcanus from various geographical regions of the Caspian Sea in Iran.…”

Section: Primers Ampli Cation and Diversity Indicessupporting

confidence: 93%

Morphological and Molecular Characterization of Iranian Wild Blackberry Species Using Multivariate Statistical Analysis and Inter-Simple Sequence Repeats (ISSR) Markers

Gharaghani

Garazhian

Eshghi

et al. 2021

Preprint

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This study was carried out to estimate the genetic diversity and relationships of 74 Iranian blackberry genotypes assigned to 5 different species using inter-simple sequence repeats (ISSR) marker analysis and morphological trait characterization. Sixteen traits including phenological, vegetative and reproductive attributes were recorded, and 10 ISSR primers were screened. Results showed that yield and leaf width have the highest and lowest genetic diversity, (diversity index = 62.57 and 13.74), respectively. Flowering and ripening date recorded as traits having the strongest correlations (r = 0.98). The selected 10 ISSR primers produced a total of 161 amplified fragments (200 to 3500 bp) of which 113 were polymorphic. The highest, lowest and average PIC values were 0.53, 0.38 and 0.44, respectively. Principle component analysis (PCA) based on morphological traits showed that the first six components explained 84.9% of the variations of traits studied, whilst the principal coordinate analysis (PCoA) based on ISSR data implied the first eight principal coordinates explained 67.06% of the total variation. Cluster analysis based on morphological traits and ISSR data classified all genotypes into two and three major groups, respectively, and the distribution pattern of genotypes was mainly based on species and the geographic origins.

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Section: Primers Ampli Cation and Diversity Indicessupporting

confidence: 93%

Morphological and Molecular Characterization of Iranian Wild Blackberry Species Using Multivariate Statistical Analysis and Inter-Simple Sequence Repeats (ISSR) Markers

Gharaghani

Garazhian

Eshghi

et al. 2021

Preprint

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“…This observation suggested that voluntary grouping on the basis of genetic relationships was also genetically meaningful. Observed degree of population differentiation in Japanese red pines (⌽ ST = 0.08) was higher than that observed in Ginkgo biloba (0.057; HONG et al, 2001) and lower than those of Torreya nucifera (0.094; HONG et al, 2000), Rubus coreanus (0.185, HONG et al, 2003), andVaccinium uliginosum (0.335, HAN et al, 2005).…”

Section: Resultsmentioning

confidence: 71%

“…Relatively high level of genetic diversity was observed within 19 populations, ranged from 0.411 (Gyeongbuk-Mungyeong) to 0.505 (Gyeongbuk-Goryeong) with the mean of 0.453 (Table 1), compared to other tree species -i.e. Torreya nucifera: 0.353 (HONG et al, 2000), Ginkgo biloba: 0.379 (HONG et al, 2001), Rubus coreanus: 0.242 (HONG et al, 2003), Vaccinium uliginosum: 0.47 (HAN et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

I-SSR Markers Revealed Inconsistent Phylogeographic Patterns among Populations of Japanese Red Pines in Korea

2007

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To monitor the level and distribution pattern of genetic diversity in countrywide populations of Japanese red pines in Korea, 80 I-SSR variants were analyzed from 192 individuals in 11 populations. The previously reported data, obtained from the 8 populations of this species, were incorporated into the estimation of the population genetic statistics. Relatively higher level of genetic diversity was observed in 19 populations of Japanese red pines (mean of 0.453) than those in other tree species. From the results of AMOVA, majority of genetic diversity (92%) was allocated within populations, which brought about the moderate degree of population differentiation (ϕST= 0.08). Two genetic groups were observed from the dendrogram reconstructed by UPGMA. Overall correlation between genetic relationships and geographic affinity was inconsistent among the 19 populations. Some probable genetic disturbances, induced by either human interference or the natural process of regeneration for the species after devastation, might be responsible for the discrepancy between genetic relationships and geographical distribution of the populations. These results suggested that simple geographic or ecological grouping might not represent the genetic zone of the Korean populations of Japanese red pines, and that more careful approach should be made for designating in situ gene conservation of this species in Korea.

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“…Based on I-SSR PCR analyses, a total of 80 variants were observed in 8 populations of Japanese red pine by using 6 I-SSR primers [UBC #809 (12 variants), 811 (11), 818 (14; Table 1). The corresponding parameters in other tree species are as follows: Torreya nucifera : 0.353 (HONG et al, 2000), Ginkgo biloba : 0.379 (HONG et al, 2001), and Rubus coreanus : 0.242 (HONG et al, 2003). Of the 8 populations, the populations of Gyeongbuk-Uljin and Chungnam-Taean turned out to be genetically most closely related (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…Furthermore, large numbers of I-SSR markers could be generated by using various kinds of primers consisted of simple sequence repeats (SSRs) motifs with a couple of additional arbitrary nucleotides at the 3'-end. The characteristic features of I-SSR enable the PCR-amplified I-SSR markers to be suitable for analyzing genetic status of the populations (SAGHAI et al, 1994;GODWIN et al, 1997;HONG et al, 2000HONG et al, , 2001HONG et al, , 2003JOSHI et al, 2000;YANG et al, 1996;PARSONS et al, 1997;SALIMATH et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Discordance Between Geographical Distribution and Genetic Relationship Among Populations of Japanese Red Pine in Korea Revealed by Analysis of I-SSR Markers

et al. 2004

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Level and distribution of genetic diversity in 8 populations of Japanese red pine in Korea were estimated using I-SSR variants. A total of 80 I-SSR variants were observed in the analyzed 150 individuals, which revealed DNA fingerprints-like individual specific amplicon profiles for all of them. Relatively higher level of genetic diversity within populations was observed in 8 populations of Japanese red pine (mean of 0.450) than in other tree species. From the results of AMOVA, majority of genetic diversity was allocated within populations (93.42%) resulting in a moderate degree of population differentiation (ΦST= 0.066). The observed distribution pattern of I-SSR variants among 8 populations was coincided with the typical patterns for the long-lived woody species. Genetic relationships among the populations, reconstructed by UPGMA and Neighbor- Joining methods, revealed 2 genetic groups. The populations of Gangwon-Uljin and Chungnam-Taean turned out to be the most closely related despite a distant location between them. The overall genetic relationships among the 8 populations, reconstructed by both methods, were not coincided with geographic distances. The discrepancy between genetic relationships and geographical distribution among the populations suggests that the analyzed populations might have undergone random changes in genetic composition due to some kinds of disturbances. Results obtained in this study suggests that more careful approach should be made in preparing strategy for gene conservation of Japanese red pine in Korea. More information on countrywide molecular population genetic status of Japanese red pine will be helpful to prepare more reasonable strategy for gene conservation of the species in the country.

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Genetic diversity in natural populations of two geographic isolates of Korean black raspberry

Cited by 15 publications

References 0 publications

Morphological and Molecular Characterization of Iranian Wild Blackberry Species Using Multivariate Statistical Analysis and Inter-Simple Sequence Repeats (ISSR) Markers

Morphological and Molecular Characterization of Iranian Wild Blackberry Species Using Multivariate Statistical Analysis and Inter-Simple Sequence Repeats (ISSR) Markers

I-SSR Markers Revealed Inconsistent Phylogeographic Patterns among Populations of Japanese Red Pines in Korea

Discordance Between Geographical Distribution and Genetic Relationship Among Populations of Japanese Red Pine in Korea Revealed by Analysis of I-SSR Markers

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