The complete chloroplast genome sequence of Hibiscus syriacus L. is presented in this study. The genome is composed of 161 019 bp in length, with a typical circular structure containing a pair of inverted repeats of 25 745 bp of length separated by a large single-copy region and a small single-copy region of 89 698 bp and 19 831 bp of length, respectively. The overall GC content is 36.8%. One hundred and fourteen genes were annotated, including 81 protein-coding genes, 4 ribosomal RNA genes and 29 transfer RNA genes.
Chimeric plants composed of green and albino tissues have great ornamental value. To unveil the functional genes responsible for albino phenotypes in chimeric plants, we inspected the complete plastid genomes (plastomes) in green and albino leaf tissues from 23 ornamental chimeric plants belonging to 20 species, including monocots, dicots, and gymnosperms. In nine chimeric plants, plastomes were identical between green and albino tissues. Meanwhile, another 14 chimeric plants were heteroplasmic, showing a mutation between green and albino tissues. We identified 14 different point mutations in eight functional plastid genes related to plastid-encoded RNA polymerase (rpo) or photosystems which caused albinism in the chimeric plants. Among them, 12 were deleterious mutations in the target genes, in which early termination appeared due to small deletion-mediated frameshift or single nucleotide substitution. Another was single nucleotide substitution in an intron of the ycf3 and the other was a missense mutation in coding region of the rpoC2 gene. We inspected chlorophyll structure, protein functional model of the rpoC2, and expression levels of the related genes in green and albino tissues of Reynoutria japonica. A single amino acid change, histidine-to-proline substitution, in the rpoC2 protein may destabilize the peripheral helix of plastid-encoded RNA polymerase, impairing the biosynthesis of the photosynthesis system in the albino tissue of R. japonica chimera plant.
Using PCR-RFLP analysis, a comparative study on the restriction site polymorphism within 8 genes and regions of the Abies chloroplast DNA has been conducted covering 15 Asian, 6 North American and 7 Mediterranean species. A variable degree of divergence was observed among individual species of a given region as well as between geographical groups. A group of the Mediterranean firs, consisting of closely related species, differed profoundly from both Asian and North American representatives. Although a higher level of restriction site variants was detected among the Asian firs, two thirds of them were allocated to the difference between A. mariesii and the other Asian firs. The North American species exhibited the highest level of polymorphism resulting in several subgroups on a cladogram. At the individual species level, the Asian species A. mariesii and the North American species A. lasiocarpa diverged conspicuously from their counterparts in their respective regions. The results of restriction site polymorphism analysis are discussed with ragard to crossability and taxonomic status of individual species.
To monitor the level and distribution pattern of genetic diversity in countrywide populations of Japanese red pines in Korea, 80 I-SSR variants were analyzed from 192 individuals in 11 populations. The previously reported data, obtained from the 8 populations of this species, were incorporated into the estimation of the population genetic statistics. Relatively higher level of genetic diversity was observed in 19 populations of Japanese red pines (mean of 0.453) than those in other tree species. From the results of AMOVA, majority of genetic diversity (92%) was allocated within populations, which brought about the moderate degree of population differentiation (ϕST= 0.08). Two genetic groups were observed from the dendrogram reconstructed by UPGMA. Overall correlation between genetic relationships and geographic affinity was inconsistent among the 19 populations. Some probable genetic disturbances, induced by either human interference or the natural process of regeneration for the species after devastation, might be responsible for the discrepancy between genetic relationships and geographical distribution of the populations. These results suggested that simple geographic or ecological grouping might not represent the genetic zone of the Korean populations of Japanese red pines, and that more careful approach should be made for designating in situ gene conservation of this species in Korea.
Level and distribution of genetic diversity in 8 populations of Japanese red pine in Korea were estimated using I-SSR variants. A total of 80 I-SSR variants were observed in the analyzed 150 individuals, which revealed DNA fingerprints-like individual specific amplicon profiles for all of them. Relatively higher level of genetic diversity within populations was observed in 8 populations of Japanese red pine (mean of 0.450) than in other tree species. From the results of AMOVA, majority of genetic diversity was allocated within populations (93.42%) resulting in a moderate degree of population differentiation (ΦST= 0.066). The observed distribution pattern of I-SSR variants among 8 populations was coincided with the typical patterns for the long-lived woody species. Genetic relationships among the populations, reconstructed by UPGMA and Neighbor- Joining methods, revealed 2 genetic groups. The populations of Gangwon-Uljin and Chungnam-Taean turned out to be the most closely related despite a distant location between them. The overall genetic relationships among the 8 populations, reconstructed by both methods, were not coincided with geographic distances. The discrepancy between genetic relationships and geographical distribution among the populations suggests that the analyzed populations might have undergone random changes in genetic composition due to some kinds of disturbances. Results obtained in this study suggests that more careful approach should be made in preparing strategy for gene conservation of Japanese red pine in Korea. More information on countrywide molecular population genetic status of Japanese red pine will be helpful to prepare more reasonable strategy for gene conservation of the species in the country.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.