Genetic diversity in Indian ash gourd (Benincasa hispida) accessions as revealed by quantitative traits and RAPD markers

Pandey, Sudhakar; Kumar, Sanjeev; Mishra, Upama; Rai, Anurag; Singh, Mahesh Kumar; Rai, Mathura

doi:10.1016/j.scienta.2008.05.031

Cited by 30 publications

(17 citation statements)

References 15 publications

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“…In spite of its high economic importance, the research on wax gourd, particularly at the levels of molecular biology and genetics, is very weak. The studies on wax gourd, to data, are only confined to a few fields, such as genetic diversity [9,10], and drug development [6,7,11]. The genomic information of wax gourd is very limited.…”

Section: Introductionmentioning

confidence: 99%

De Novo Assembly and Characterization of the Transcriptome, and Development of SSR Markers in Wax Gourd (Benicasa hispida)

et al. 2013

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BackgroundWax gourd is a widely used vegetable of Cucuribtaceae, and also has important medicinal and health values. However, the genomic resources of wax gourd were scarcity, and only a few nucleotide sequences could be obtained in public databases.Methodology/Principal FindingsIn this study, we examined transcriptome in wax gourd. More than 44 million of high quality reads were generated from five different tissues of wax gourd using Illumina paired-end sequencing technology. Approximately 4 Gbp data were generated, and de novo assembled into 65,059 unigenes, with an N50 of 1,132 bp. Based on sequence similarity search with known protein database, 36,070 (55.4%) showed significant similarity to known proteins in Nr database, and 24,969 (38.4%) had BLAST hits in Swiss-Prot database. Among the annotated unigenes, 14,994 of wax gourd unigenes were assigned to GO term annotation, and 23,977 were found to have COG classifications. In addition, a total of 18,713 unigenes were assigned to 281 KEGG pathways. Furthermore, 6,242 microsatellites (simple sequence repeats) were detected as potential molecular markers in wax gourd. Two hundred primer pairs for SSRs were designed for validation of the amplification and polymorphism. The result showed that 170 of the 200 primer pairs were successfully amplified and 49 (28.8%) of them exhibited polymorphisms.Conclusion/SignificanceOur study enriches the genomic resources of wax gourd and provides powerful information for future studies. The availability of this ample amount of information about the transcriptome and SSRs in wax gourd could serve as valuable basis for studies on the physiology, biochemistry, molecular genetics and molecular breeding of this important vegetable crop.

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Section: Introductionmentioning

confidence: 99%

De Novo Assembly and Characterization of the Transcriptome, and Development of SSR Markers in Wax Gourd (Benicasa hispida)

et al. 2013

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“…Despite what Peter et al (1991) claimed about low diversity of the ash gourd gene pool, Pandey et al (2008) reported that in a study based on molecular markers quite big diversity of the ash gourd germplasm was detected; on the other hand, Sureja et al (2006) reported small diversity of the ash gourd germplasm they studied. The diversity of physiological and crop traits in our research was very large (see Fig.…”

Section: Discussionmentioning

confidence: 85%

“…For creating variability, crossing among parental lines is a very efficient method. However, selection of divergent parents is important because the greater is the genetic divergence among the parents for the characters, the better are the chances of obtaining a pool of genotypes of large diversity (Pandey et al 2008;Verma et al 2007). At the same time, of course, genotypes possessing desirable traits are important.…”

Section: Introductionmentioning

confidence: 99%

Searching for the optimum multivariate profile of an ash gourd [Benincasa hispida (Thunb.) Cogn.] genotype

2010

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Ash gourd is an important vegetable especially in the Indo-China region, but also in America and Africa. It is a monotypic genus, and its genetic pool is considered little diverse, which makes breeding of good ash gourd cultivars difficult. A pool of 56 genotypes, of which 10 are released cultivars and 46 indigenous lines collected from different parts of India, was studied in a two-year experiment. Various traits are important when breeding new cultivars, so an optimum multivariate performance is studied in this germplasm, with a special focus on traits of the greatest importance, namely fruit yield per plant, flesh thickness, vine length, and number of days to female and male flowering. Such performance is studied for the bestyielding genotypes, and promising genotypes in terms of multivariate performance are selected.

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“…As a result, markers like RAPDs may accurately assay the degree of genetic change between two genomes, but they may not necessarily reflect the divergence in terms of changes in traits of agronomic importance. Pandey et al (2008) showed that the comparison of Jaccard's similarity based on RAPD markers and average taxonomic distance based on quantitative characters was not significant (r = 0.0477) in Indian ash gourd.…”

Section: Genetic and Chemical Analysis Of Cowpeamentioning

confidence: 92%

“…The agronomic characterization and RAPD based on dendrogram were somewhat similar, indicating that the agronomic characterizing information will continue to be useful to identify diverse germplasm in breeding programs of lupine and/or other species (Abd EL- Ghani et al 2007). Pandey et al (2008) showed that DNA markers are preferable to morphological ones because they relate variability directly at the genetic level and provide reliable and enormous data that permit a reproducible estimate of genetic diversity in the germplasm.…”

Section: Cluster Analysis Based On Resistance To Weevil Pest Traits Omentioning

confidence: 99%

Genetic and chemical analyses of six cowpea and two Phaseolus bean species differing in resistance to weevil pest

Abdel-Sabour

Obiadalla-Ali

AbdelRehim

2010

J. Crop Sci. Biotechnol.

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The objective of this work was to evaluate the genetic variability among six cowpea (Vigna unguiculata) cultivars differing in their resistance to Callosobruchus maculatus (F.) weevil. Two resistant bean cultivars were used to compare between the sensitive, moderate tolerant, and high tolerant cowpea cultivars. The differentiations were performed by using random amplified polymorphic DNA (RAPD) fingerprinting, protein concentration and organic and non-organic components in seed coat. Six polymorphic primers were identified, resulting in different informative bands. Based on polymorphic profiles, three clusters were formed. Clustering was mainly affected by the resistance to weevil pest. The sensitive cowpea cultivars were separated in one group, the moderate tolerant and high tolerant cultivars came in separate groups, and finally, the resistant bean cultivars separated clearly in one distinct group. The most interesting result was represented by concentration of total protein in the seed coat. The protein concentration in the resistant bean cultivars were approximately 50% less than concentration in each of the moderate tolerant and sensitive cultivars of cowpea. Ferric ions were about 25% less than the moderate tolerant and sensitive cultivars. The concentrations of calcium and potassium in seed coats were higher in the resistant beans than in cowpea cultivars. Cobalt was about four times higher in resistant bean than in the sensitive and moderate tolerant cowpea cultivars, which may play a major role in seed resistance to weevil.

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Genetic diversity in Indian ash gourd (Benincasa hispida) accessions as revealed by quantitative traits and RAPD markers

Cited by 30 publications

References 15 publications

De Novo Assembly and Characterization of the Transcriptome, and Development of SSR Markers in Wax Gourd (Benicasa hispida)

De Novo Assembly and Characterization of the Transcriptome, and Development of SSR Markers in Wax Gourd (Benicasa hispida)

Searching for the optimum multivariate profile of an ash gourd [Benincasa hispida (Thunb.) Cogn.] genotype

Genetic and chemical analyses of six cowpea and two Phaseolus bean species differing in resistance to weevil pest

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