GENETIC DIVERSITY IN ACCESSIONS OF Passiflora cincinnata Mast. BASED ON MORPHOAGRONOMIC DESCRIPTORS AND MOLECULAR MARKERS

Abstract

-Passiflora cincinnata Mast. has become more popular in the market because the unusual flavor of its fruits and natural beauty of its flowers, and has great potential for breeding programs of Passiflora edulis f. flavicarpa, because its resistance to diseases and drought. The objective of this work was to evaluate seven wild passion fruit (P. cincinnata) accessions, using morphological and agronomic descriptors and molecular markers type ISSR, to identify their morphoagronomic and genetic variabilities and potential for use in breeding programs. A randomized block experimental design was used with five replications and two plants per plot. Thirteen qualitative and twenty-one quantitative, vegetative and floral characteristics were used for morphoagronomic characterization. Twelve ISSR primers were evaluated for molecular characterization. Among the qualitative characteristics, only the color variations were significantly different between the accessions. According to the mean squares of the quantitative characteristics evaluated, obtained from analysis of variance, the means of accessions showed significant differences (p<0.01) for all characteristics. The IAL (internode average length) was the morphological descriptor that most contributed to diversity, with 43.12%, followed by DH5 (stem diameter at 5 cm height) and SW (sepal width). The average genetic similarity found was 68%. Despite the low genetic variability found among accessions, the primers UBC-887 and UBC-841 stood out with high percentage of polymorphism with 14 and 11 polymorphic fragments, respectively, and higher values of polymorphism information content (PIC), resolving power (RP) and marker index (MI), denoting suitability for use in diversity studies of P. cincinnata. Low variability was found among accessions evaluated.Keywords: Genetic Resour ces. Plant Br eeding. ISSR. Passiflor aceae. DIVERSIDADE GENÉTICA EM ACESSOS DE Passiflora cincinnata Mast. BASEADA EM DESCRITORES MORFOAGRONÔMICOS E MARCADORES MOLECULARESRESUMO -A espécie Passiflora cincinnata Mast. vem se popularizando no mercado pelo sabor incomum dos seus frutos, beleza natural de sua flores e possui grande potencial para a cultura de Passiflora edulis f. flavicarpa, pois apresenta resistência a doenças e déficit hídrico. Este trabalho teve como objetivo avaliar sete acessos de maracujá-do-mato (P. cincinnata) por meio de descritores morfológicos, descritores agronômicos e marcadores moleculares do tipo ISSR visando identificar variabilidade morfoagronômica e genética e o potencial para serem utilizados em programas de melhoramento. O delineamento experimental foi em blocos casualizados, com cinco repetições e duas plantas por parcela. Para caracterização morfoagronômica foram avaliadas 13 características qualitativas e 21 características quantitativas vegetativas e florais. Para caracterização molecular foram testados 12 primers de ISSR. Entre as características qualitativas apenas as variações de coloração apresentaram diferenças marcantes entre os diferentes acessos. De...

Characterization of the genetic diversity in Passiflora spp. in the Boyacá Department, Colombia

“…The coefficient of cofenetic correlation was 75%, demonstrating a relationship between the genetic distances and the groups. Similar results were obtained by Batista et al (2017) when evaluating genetic diversity in P. cincinnata accessions based on morphological descriptors and molecular markers and by Oliveira et al (2019) when evaluating the genetic diversity of Passiflora spp. using ISSR and RAPD markers.…”

“…In studies on genetic diversity in Passiflora, it has been found that ISSR markers are highly polymorphic and informative (Batista et al, 2017;Oliveira et al, 2019;Vianna et al, 2019); in addition, heterozygosity values greater than those found in this study have been reported by Lougon et al (2014) when evaluating the genetic diversity in the Passiflora genus with SSR markers, finding an average heterozygosity of He = 0.57. These values show broad genetic variability in the Passiflora genus.…”

“…The genetic diversity in Passiflora has been studied with different molecular techniques, such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) (Oluoch et al, 2018;Vieira et al, 2019); however, simple sequence repeat (SSR) markers are the most used techniques for the characterization of germplasm in different crops since they are highly polymorphic, reproducible and co-dominant, with wide genome coverage (Gramazio et al, 2018). Among the microsatellite markers, inter-simple sequence repeat (ISSR), which combines characteristics of the RAPD and microsatellites techniques, facilitates multilocus genomic analysis with a single primer based on regions of SSR, presents good reproducibility, has a low cost, is simple, does not need previous genome information (Morillo et al, 2018), and has proven to be polymorphic and informative in studies on genetic diversity in Passiflora (Santos et al, 2011;Sousa et al, 2015;Batista et al, 2017;Oliveira et al, 2019;Vianna et al, 2019).…”

Genetic diversity in wild populations of the restinga ecotype of the cashew (Anacardium occidentale) in coastal Piauí, Brazil

“…We used ISSR molecular markers (inter-simple sequence repeat), widely deployed in studies of economically important species and their wild relatives that focus on genotype identification, genetic conservation and cultivar development (e.g. Nunes et al 2013;Martins et al 2014;Oliveira et al 2014;Rodrigues et al 2015;Carmo et al 2017;Wu et al 2019).…”

O maracujazeiro-do-mato (Passiflora Cincinnata mast.) e sua importância econômica: Uma revisão narrativa

“…No entanto, a potencialidade agronômica de espécies silvestres de maracujazeiros não foi completamente explorada por motivos de escassez em pesquisas básicas e aplicadas (Santos et al, 2012). Um destes potenciais pode ser encontrado na espécie Passiflora cincinnata Mast., que tem se popularizado no mercado por ser uma espécie nativa na região semiárida, pelo sabor incomum de seus frutos, por sua beleza natural e de suas flores e também pela resistência a doenças e ao déficit hídrico (Carmo et al, 2017).…”