Genetic diversity based on MIRU-VNTR profile of isolates of Mycobacterium bovis from Mexican cattle

Vargas, Alejandro Nava; Suazo, Feliciano Milián; Alarcón, Germinal Jorge Cantó; Venegas, Yezenia Rubio; Solorio, Roberto Guerrero; Rodríguez-Hernández, Elba; Pizano-Martínez, Óscar

doi:10.1016/j.prevetmed.2016.07.007

Cited by 8 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The finding that MIRU31 had the highest discriminatory power was in line with those of Jeon et al (2008), Besirovic et al (2012), andBolado-Martínez et al (2015), while Supply et al (2006) found the discriminatory power of this locus to be medium and El Sayed (2019) reported this locus to have the lowest discriminatory power. MIRU26 was reported to have the second highest discriminatory power, which was in line with the findings of several researchers (Hilty et al 2005;Allix et al 2006Boniotti et al 2009;Parreiras et al 2012;Laniado-Laborín et al 2014;Bolado-Martínez et al 2015;Vargas et al 2016), who reported the same result. Another locus with discriminatory power was MIRU10, in line with the findings of Besirovic et al (2012), andElsayed (2019) while Bolado-Martínez et al (2015) and Vargas et al (2016) reported that this locus had only medium discriminatory power.…”

Section: Discussionsupporting

confidence: 90%

“…With the emergence of molecular epidemiology, faster and more reliable diagnostic methods were developed that provided information about the relationships between the strains as well as the genetic structure. MIRU-VNTR is a molecular tool that makes use of fast, objective, and high-resolution monitoring procedures for the identification of outbreak dynamics and epidemiology (Vargas et al 2016). The goal of the present study is to determine the epidemiological characteristics of the clinical isolates of M. bovis in Konya province at a molecular level.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Typing of Mycobacterium bovis isolates from cattle using MIRU-VNTR analysis

Gülcü

Hadimli

2020

Etlik Veteriner Mikrobiyoloji Dergisi

View full text Add to dashboard Cite

Bovine tuberculosis, as a significant threat to both animal and human health, is a common global zoonotic disease. The emergence of molecular epidemiology has made it possible to gain a better understanding of the dynamics of disease transmission, and consequently, to come up with more effective control methods. The present study seeks to identify Mycobacterium bovis isolates in our region at a genotype level. To this end, the molecular epidemiological characteristics of Mycobacterium bovis strains isolated using classical methods and identified using molecular methods from the tissues and organs of cattle with suspected tuberculosis, obtained from slaughterhouses in the Konya province, or from those sent to Konya Veterinary Control Institute, were determined through genotyping. In the analysis of a total of 70 Mycobacterium bovis isolates, carried out using the MIRU-VNTR method, it was found that the repeat numbers for MIRU2, MIRU4, MIRU20, MIRU23, MIRU24, MIRU27, and MIRU39 loci did not vary between strains, while the repeat numbers for MIRU10, MIRU16, MIRU26, MIRU31, and MIRU40 loci varied between strains and had a high discriminatory power (0.25≤h). It was further observed that 29 subgroups between 1-14 isolates formed. The movement of animals in our region, which occurs for several reasons, is considered to cause Mycobacterium bovis strains to vary between herds, and the fact that the cattle from which the isolates were sourced for our study came from different herds was considered to cause a variation in the discriminatory power in the MIRU loci.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Typing of Mycobacterium bovis isolates from cattle using MIRU-VNTR analysis

Gülcü

Hadimli

2020

Etlik Veteriner Mikrobiyoloji Dergisi

View full text Add to dashboard Cite

show abstract

“…In Mexico, typing of M . bovis has been done by RFLP, RAPD-PCR, spoligotyping, and MIRU-VNTR [ 8 , 10 , 11 , 43 , 44 ]. Spoligotyping is cheap, easy to perform, it requires minimal amount of DNA and there is an international database where spoligotypes can be reported, stored and compared [Smith et al, 2012].…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping

et al. 2018

Self Cite

View full text Add to dashboard Cite

Mycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genotyping of M. bovis has been performed mainly by spoligotyping and variable number tandem repeats (VNTR), but higher resolution power can be useful for a finer definition of the spread of the disease. Whole genome sequencing and spoligotyping was performed for a set of 322 M. bovis isolates from different sources in Mexico: Baja California, Coahuila, Estado de Mexico, Guanajuato, Hidalgo, Jalisco, Queretaro and Veracruz, from dairy and beef cattle, as well as humans. Twelve main genetic clades were obtained through WGS and genetic diversity analysis. A clear differentiation of the Baja California isolates was seen as they clustered together exclusively. However, isolates from the central states showed no specific clustering whatsoever. Although WGS proves to have higher resolving power than spoligotyping, and since there was concordance between WGS and spoligotyping results, we consider that the latter is still an efficient and practical method for monitoring bovine tuberculosis in developing countries, where resources for higher technology are scarce.

show abstract

“…The number of MIRUs per genome is around 40-50. VNTR typing is useful for epidemiological studies and can be used to recognize multiple infections [ 204 , 205 ].…”

Section: Partial-genome Typing Of Mtbcmentioning

confidence: 99%

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

et al. 2020

View full text Add to dashboard Cite

Tuberculosis (TB) is a chronic inflammatory and zoonotic disease caused by Mycobacterium tuberculosis complex (MTBC) members, which affects various domestic animals, wildlife, and humans. Some wild animals serve as reservoir hosts in the transmission and epidemiology of the disease. Therefore, the monitoring and surveillance of both wild and domestic hosts are critical for prevention and control strategies. For TB diagnosis, the single intradermal tuberculin test or the single comparative intradermal tuberculin test, and the gamma-interferon test, which is regarded as an ancillary test, are used. Postmortem examination can identify granulomatous lesions compatible with a diagnosis of TB . In contrast, smears of the lesions can be stained for acid-fast bacilli, and samples of the affected organs can be subjected to histopathological analyses. Culture is the gold standard test for isolating mycobacterial bacilli because it has high sensitivity and specificity compared with other methods. Serology for antibody detection allows the testing of many samples simply, rapidly, and inexpensively, and the protocol can be standardized in different laboratories. Molecular biological analyses are also applicable to trace the epidemiology of the disease. In conclusion, reviewing the various techniques used in MTBC diagnosis can help establish guidelines for researchers when choosing a particular diagnostic method depending on the situation at hand, be it disease outbreaks in wildlife or for epidemiological studies. This is because a good understanding of various diagnostic techniques will aid in monitoring and managing emerging pandemic threats of infectious diseases from wildlife and also preventing the potential spread of zoonotic TB to livestock and humans. This review aimed to provide up-to-date information on different techniques used for diagnosing TB at the interfaces between wildlife, livestock, and humans.

show abstract

Genetic diversity based on MIRU-VNTR profile of isolates of Mycobacterium bovis from Mexican cattle

Cited by 8 publications

References 13 publications

Typing of Mycobacterium bovis isolates from cattle using MIRU-VNTR analysis

Typing of Mycobacterium bovis isolates from cattle using MIRU-VNTR analysis

Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

Contact Info

Product

Resources

About