Genetic diversity assessment using RAPD primers in insecticide resistant populations of diamondback moth Plutella xylostella (Linn.)

Abstract

Genetic diversity in acephate, spinosad and Cry2Ab resistant Plutella xylostella collected from three states of India was assessed by RAPD markers. The DNA extracted from larvae was subjected to polymerase chain reaction using 10 RAPD primers. The highest number alleles (7) were produced by primer ABA-13, followed by six alleles each by primers ABA-2, 7, 8, 11, 14; five alleles each were produced by ABA-4, 9, 10, 12. UPGMA analysis clustered the acephate, spinosad and Cry2Ab treated P.xylostella populations into two groups with overall similarity level of 33%, 27% and 34% respectively. Cluster A consisted 11 samples while Cluster B consisted only F 1 of acephate and spinosad treated Karnataka population. In Cry2Ab treated population Cluster B comprised 11 samples and Cluster A had out grouped singly i.e. F 0 generation from Karnataka. The genetic variability between the acephate, spinosad and Cry2Ab treated populations ranged from 33 to 69%, 27 to 56% and 34 to 69% respectively. Acephate and spinosad treated F 1 population and Cry2Ab treated F 0 population from Karnataka were out grouped from rest of the populations.

Detection of Genetic Relationship and Genetic Variation of the Four Species of Real Flies Using Molecular Markers

“…The study also showed the presence of distinct bands (Absent bands, Unique bands) as shown in Table ( 6). The total of distinct bands resulting from the primers was (85) bands of which (67) were unique bands and (18) bands were absent. , The metal fly was characterized by the highest percentage of unique bands, reaching (24) bands, while the horse fly got the lowest percentage of unique bands, reaching (12) bands.…”

“…Researchers have sought to develop these markers and normalize them to work since their discovery until now (Williams et al, 1990). Many of these markers, especially RAPD, have been widely used in studying the genetic variation between flies and determining the genetic signature of flies (Singh et al, 2016: Sunitha et al, 2015: Malviya et al, 2010. The aim of the research is to uncover the genetic relationship and the genetic variation of four types of real flies using molecular markers.…”

Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers

“…This molecular marker has an advantage of the possibility of using a different set of primers and choosing the best for each analysis as different sets of primers often produce different banding patterns that can be used in a variety of studies (Yulin et al 1998, grutzmacher et al 2007, Johnson et al 2008, Zahoor et al 2013, Coelho-Bortolo et al 2016, Preet and gupta 2017. Therefore, these markers have several entomological applications, for example, in forensic entomology, breeding programs, taxonomic identification of pests of agronomic interests, detection of genetic variation among populations, as well as, in the identification of closely related species (Grzywacz et al 2012, ercan et al 2012, Sivasankaran et al 2013, Matta et al 2013, Sunitha et al 2015, Singh et al 2016, Haghighi and Kumar 2017, Queiroz et al 2017, Sharma and Singh 2017. With regard to species identification, the analysis of electrophoretic profiles has been used in other groups of insects, with satisfactory results (Yulin et al 1998, 1999, Johnson et al 2008, Sivasankaran et al 2013, Zahoor et al 2013, Bajpai 2016a.…”

Antifeedant effects of crofton weed,Ageratina adenophoraextracts and their phytochemical compounds against three cabbage insect pest species

“…Pieris brassicae is also considered very destructive, as it can damage the host plant from seedling to vegetative and flowering stages (Younas et al, 2004). In India, P. xylostella was reported as a major pest by 92% of farmers, with losses of up to 50% (Grzywacz et al, 2010; Sunitha et al, 2020; Singh et al, 2021). Female moths can lay up to about 200 eggs on the upper leaf surface (Shakeel et al, 2017).…”