Genetic diversity assessment of sorghum (Sorghum bicolor (L.) Moench) accessions using single nucleotide polymorphism markers

Abstract

Sorghum (Sorghum bicolor (L.) Moench) is an important resource to the national economy and it is essential to assess the genetic diversity in existing sorghum germplasm for better conservation, utilization and crop improvement. The aim of this study was to evaluate the level of genetic diversity within and among sorghum germplasms collected from diverse institutes in Nigeria and Mali using Single Nucleotide Polymorphic markers. Genetic diversity among the germplasm was low with an average polymorphism information content value of 0.24. Analysis of Molecular Variation revealed 6% variation among germplasm and 94% within germplasms. Dendrogram revealed three groups of clustering which indicate variations within the germplasms. Private alleles identified in the sorghum accessions from National Center for Genetic Resources and Biotechnology, Ibadan, Nigeria and International Crop Research Institute for the Semi-Arid Tropics, Kano, Nigeria shows their prospect for sorghum improvement and discovery of new agronomic traits. The presence of private alleles and genetic variation within the germplasms indicates that the accessions are valuable resources for future breeding programs.

Hybrid performance as related to genomic diversity and population structure in public sorghum inbred lines

“…The PIC value obtained in the current study is comparable with other results, such as the recent work by Afolayan et al. (2019). Furthermore, given that the current study is based on elite breeding lines subjected to intensive selection, the level of diversity observed was considered robust.…”

“…However, the materials included in the study not only represent small fraction of this diversity but were also subjected to inbreeding and selection. The PIC value obtained in the current study is comparable with other results, such as the recent work by Afolayan et al (2019). Furthermore, given that the current study is based on elite breeding lines subjected to intensive selection, the level of diversity observed was considered robust.…”

Genetic Diversity and Population Structure of Sorghum [Sorghum Bicolor (L.) Moench] Accessions as Revealed by Single Nucleotide Polymorphism Markers

“…In the case of bi-allelic SNP markers, the maximum PIC value of 0.375 is attained when both alleles have a frequency of 0.5. In the present bi-allelic SNP-based study, the PIC values ranged from 0.09 to 0.375 with the overall average of 0.24, which is comparable with previous studies on sorghum using SNP markers ( Afolayan et al, 2019 ; Silva et al, 2021 ; Wondimu et al, 2021 ). Forty-seven percent of these SNP loci have a PIC value of greater than 0.25 and hence they are highly informative and could be used for various applications including population genetic studies of sorghum.…”

“…The genetic diversity of crop species can be studied through morphological, biochemical, and molecular markers ( Rao et al, 1996 ; Geleta and Labuschagne, 2005 ; Mehmood et al, 2008 ; Enyew et al, 2021 ). Previous studies on the genetic diversity of sorghum have been carried out by using random amplified polymorphism DNA (RAPD) analysis ( Ayana et al, 2000 ; Ruiz-Chután et al, 2019 ), simple sequence repeat (SSR) markers ( Djè et al, 2000 ; Ghebru et al, 2002 ; Manzelli et al, 2007 ; Ali et al, 2008 ; Wang et al, 2009 ; Ng’uni et al, 2011 , 2012 ; Burow et al, 2012 ; Adugna et al, 2013 ; Adugna, 2014 ; Mofokeng et al, 2014 ; Motlhaodi et al, 2014 , 2017 ), and express sequence tags (EST) SSR markers ( Ramu et al, 2013 ), SNP markers ( Cuevas et al, 2017 ; Afolayan et al, 2019 ; Cuevas and Prom, 2020 ). More recently, a few studies have been performed on the genetic diversity of Ethiopian sorghum accessions using SNP markers ( Girma et al, 2019 ; Menamo et al, 2021 ; Wondimu et al, 2021 ).…”

Understanding the Sorghum–Colletotrichum sublineola Interactions for Enhanced Host Resistance

“…To this end, improving anthracnose resistance has been an utmost priority in sorghum breeding programs. Fortunately, there is a high genetic diversity and wide anthracnose resistance variation in sorghum landraces (Motlhaodi et al, 2017;Afolayan et al, 2019;Cuevas and Prom, 2020;Mengistu et al, 2020), which can be explored and used in its breeding program for improving resistance against the disease.…”