Genetic diversity and structure of the endangered species Megaleranthis saniculifolia in Korea as revealed by allozyme and ISSR markers

Jeong, Ji Hee; Kim, Eun Hye; Guo, Weihua; Yoo, Ki Oug; Jo, Dong Gwang; Kim, Zin Suh

doi:10.1007/s00606-010-0333-y

Cited by 28 publications

(16 citation statements)

References 37 publications

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“…The allozyme and ISSR markers might have been influenced by evolutionary factors such as selection or mutation in different ways because both markers are related to spatially and functionally different genomic regions (Torres et al 2003). Many previous studies using isozymes or other DNA markers, such as ISSR or RAPD, have shown similar results (Torres et al 2003;Jeong et al 2010). In this study, there was a high correlation between diversity indices of the populations based on both allozyme and ISSR markers.…”

Section: Discussionsupporting

confidence: 75%

Genetic diversity of four new species related to southwestern Sichuan buckwheats as revealed by karyotype, ISSR and allozyme characterization

et al. 2012

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The genetic diversity of four new species related to southwestern Sichuan buckwheats was examined using karyotypes, allozymes and intersimple sequence repeats (ISSR). Karyotype analysis showed that Fagopyrum wenchuanense, F. qiangcai and F. pugense are diploid (2n = 2x = 16) while F. crispatofolium is tetraploid (2n = 4x = 32). The karyotype evolutionary trend of buckwheat indicated that F. crispatofolium, F. gracilipes var. odontopterum and F. gracilipes seemed to be more advanced than F. wenchuanense, F. qiangcai, F. pugense, F. esculentum, F. cymosum and F. tataricumare. Three polymorphic enzyme systems were used for the examination of variation, including peroxidase, esterase and superoxide dismutase. Consistent banding patterns were obtained for esterase and superoxide dismutase, while peroxidase produced a large number of sharp bands. A total of 18 ISSR primers were selected for the analysis and showed high variations among the species. Allozymes and ISSR markers were utilized to estimate the genetic distance among accessions and to draw phylogenetic trees. Our data provide evidence of a high degree of genetic diversity among southwestern Sichuan buckwheats. In addition, both cultivars and wild types showed a high degree of divergence suggesting a complex domestication process in this crop. This study provides a better understanding of evolutionary mechanisms and genetic relationships in four new species of buckwheat.

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Section: Discussionsupporting

confidence: 75%

Genetic diversity of four new species related to southwestern Sichuan buckwheats as revealed by karyotype, ISSR and allozyme characterization

et al. 2012

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“…fragmented, endemic and rare) we had predicted that there should be low genetic diversity, but generally speaking, it seems that the total genetic diversity based on ISSR and AFLP markers is similar to, or slightly higher, than most of those described by different authors in other species. In fact, Nei's gene diversity estimates in other AFLP studies ranged from 0.07-0.26 (Gaudeul et al 2000;Rottenberg & Parker 2003;Barnaud & Houliston 2010;Chen et al 2010;Gong et al 2010) and in the case of ISSR analysis ranged from 0.10-0.28 (Qiu et al 2004;Xiao et al 2004;Wu et al 2004;Shao et al 2009;Jeong et al 2010). In addition, determining levels of diversity within species and populations, as well as ascertaining how species diversity is apportioned within and among populations, is useful in formulating strategies for conserving diversity within taxa (Hamrick et al 1991).…”

Section: Genetic Diversitymentioning

confidence: 92%

Genetic diversity and structure in fragmented populations of the endangered species Ranunculus cabrerensis (Ranunculaceae): implications for conservation

2012

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Ranunculus cabrerensis is an endemic and endangered species of the Northwestern Iberian Peninsula. The molecular markers AFLP and ISSR were used to investigate the genetic diversity and population structure of four populations across its known distribution. Fifteen selective primer combinations of AFLP and seventeen ISSR primer combinations produced a total of 2830 and 103 unambiguously repeatable fragments respectively, of which 97.57 and 81.38% were polymorphic for both markers. The genetic diversity of R. cabrerensis at species level was high (HE = 0.294 by ISSR and HE = 0.191 by AFLP) and differentiation between sampled locations was also relatively high (GST = 0.316 and 0.158 by ISSR and AFLP analysis respectively) compared to other studies of endangered and rare species using the same techniques. The analysis of molecular variance (AMOVA) indicated that the main genetic variation was within sampled locations (73% by AFLP; 52% by ISSR), even though the variation among locations was also significant. Principal Coordinates, NeighborNet and Bayesian analyses revealed a weak but significant relationship between the genetic structures of different populations in R. cabrerensis, with gene flow acting as a homogenizing force that prevents stronger differentiation of populations. Finally, suggestions for conservation strategies to preserve the genetic resources of this species are outlined.

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“…In some cases, the genetic diversity of a fragmented population can be higher than that of a continuously distributed population (Llorens et al 2004). This is because the effects of habitat fragmentation on genetic diversity and population structure can be affected by other factors, such as population size, gene flow, and the time scale of fragmentation (Jeong et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation and structure of Juniperus chinensis L. (Cupressaceae) in Korea

Kim

Shin

Jeong³

et al. 2018

j ecology environ

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Background: Juniperus chinensis L. populations are distributed locally on several areas including coastal cliffs which are difficult to access in the central eastern Korea. Wild populations inhabit relatively barren environments such as rocky areas and cliffs, which are very sensitive to even minor environmental disturbances including artificial interventions and natural disturbances, and thus demonstrate great fluctuations in the population size and density. This study aims to analyze the genetic diversity, differentiation, and genetic structure of each population in order to provide useful data required to establish a substantial conservation strategy of J. chinensis. Results: The genetic diversity of J. chinensis at the population level (P = 78.7%, h = 0.282, S.I. = 0.420) was somewhat higher compared with those measured in the same genus, Juniperus. The genetic differentiation degree among nine populations established naturally in central eastern Korea was 11.50% and that among sub-populations within the same area was 5.52%. On the other hand, genetic variation of individuals within the populations was 82.93%. But frequency of the main allele was different among loci. In particular, fixation of allele frequency and occurrence of rare allele in the highly isolated population suggest a likelihood that genetic drift would occur in populations of this plant. As the result of analysis on the genetic structure of nine populations, nearby populations and isolated populations tended to form separate clusters from each other as the hypothetical number of clusters (K) increase. Conclusions: This result implies that if the population size of J. chinensis is reduced due to environmental change and artificial and/or natural disturbances in the future, it could affect negatively on the genetic diversity of the plant species. In order to maintain and conserve genetic diversity of J. chinensis, ecological network, which can help genetic exchange among the local populations, should be prepared, and conservation strategies in situ as well as ex situ are also required with continuous monitoring.

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Genetic diversity and structure of the endangered species Megaleranthis saniculifolia in Korea as revealed by allozyme and ISSR markers

Cited by 28 publications

References 37 publications

Genetic diversity of four new species related to southwestern Sichuan buckwheats as revealed by karyotype, ISSR and allozyme characterization

Genetic diversity of four new species related to southwestern Sichuan buckwheats as revealed by karyotype, ISSR and allozyme characterization

Genetic diversity and structure in fragmented populations of the endangered species Ranunculus cabrerensis (Ranunculaceae): implications for conservation

Genetic variation and structure of Juniperus chinensis L. (Cupressaceae) in Korea

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