Genetic diversity of four new species related to southwestern Sichuan buckwheats as revealed by karyotype, ISSR and allozyme characterization

Zhou, Meiliang; Bai, Da-Quan; Tang, Yu; Zhu, Xuemei; Shao, Ji-Rong

doi:10.1007/s00606-011-0586-0

Cited by 28 publications

(14 citation statements)

References 28 publications

(39 reference statements)

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“…The unique characteristic of F. crispatifolium is rugged texture of leaf surface. Molecular systematic studies showed that the species is closely related to F. gracilipes and F. pugense (Jin et al 2018, Zhou et al 2012.…”

Section: Recent Discovery Of the New Species Of Fagopyrum And Their Smentioning

confidence: 99%

Classification and systematics of the <i>Fagopyrum</i> species

Ohsako

2020

Breed. Sci.

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Fagopyrum (Polygonaceae) is a small genus including less than 30 species, mostly endemic to southern China. The genus includes two cultivated species, common buckwheat F. esculentum and Tartary buckwheat F. tataricum. Fagopyrum is clearly distinguished from other genus in Polygonaceae by the central position of embryo in achene. The genus is divided into two major groups, namely the cymosum group and the urophyllum group, based on morphological characters and molecular systematics. In the last three decades the number of species in the genus has doubled by the discovery of new species by Japanese and Chinese groups. Most of them are revealed to be included in the urophyllum group based on morphological and molecular genetic analyses. Molecular systematic surveys have also detected inappropriate treatment of some non-Fagopyrum species as new species or combination in the genus.

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Section: Recent Discovery Of the New Species Of Fagopyrum And Their Smentioning

confidence: 99%

Classification and systematics of the <i>Fagopyrum</i> species

Ohsako

2020

Breed. Sci.

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“…megaspartanium of the large achene group were very similar to the two cultivated species in many aspects and were considered the potential ancestors of common buckwheat (F. esculentum) and tartary buckwheat (F. tataricum) respectively (Steward, 1930;Ohnishi, 2016). However, reports based on morphological cladistics, isozyme analysis, and DNA polymorphism suggested that F. cymosum is not the direct ancestor of buckwheat; it is only distantly related to F. tataricum and F. esculentum (Ohnishi and Matsuoka, 1996;Kadyrova et al, 2010;Zhou et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Fagopyrum esculentum ssp. ancestrale-A Hybrid Species Between Diploid F. cymosum and F. esculentum

et al. 2020

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Fagopyrum cymosum is considered as most probable wild ancestor of cultivated buckwheat. However, the evolutionary route from F. cymosum to F. esculentum remains to be deciphered. We hypothesized that a hybrid species exists in natural habitats between diploid F. cymosum and F. esculentum. The aim of this research was to determine the phylogenetic position of F. esculentum ssp. ancestrale and to provide new thoughts on buckwheat evolution. Different methodologies including evaluation of morphological traits, determination of secondary metabolites, fluorescence in situ hybridization (FISH), comparative chloroplast genomics, and molecular markers were deployed to determine the phylogenetic relationship of F. esculentum ssp. ancestrale with F. cymosum and F. esculentum. The ambiguity observed in morphological pattern of genetic variation in three species revealed that F. esculentum ssp. ancestrale is closely related to F. cymosum and F. esculentum. Flavonoid analysis revealed that F. esculentum ssp. ancestrale is closely related to F. esculentum. Comparative chloroplast genome analysis further supported the close proximity of F. esculentum ssp. ancestrale with F. esculentum. Additionally, molecular marker analysis revealed that F. esculentum ssp. ancestrale exhibits co-dominance with the bands amplified by F. cymosum and F. esculentum. These finding provided supporting evidence in favor of the hypothesis that F. esculentum ssp. ancestrale is a hybrid species between F. cymosum to F. esculentum, which was probably originated by spontaneous hybridization under natural conditions.

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“…Until now, numerous researchers reported that germplasm resources have been innovated using polyploidization techniques in many crop or medicinal plants (Ahloowalia 1967; Kermani et al 2003; Tang et al 2010; Ye et al 2010; Kanoktip et al 2014) including F. esculentum (Zhu and Gao 1988; Lian and Chen 2013). Limited works regarding tartary buckwheat, polyploidy induction works have been reported (Hou et al 2002; Zhou et al 2012). In the present study, the tetraploid F. tataricum plants were successfully induced by colchicine.…”

Section: Introductionmentioning

confidence: 99%

Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Fagopyrum tataricum (L.) Gaertn

Wang

Sheng

Wen³

et al. 2017

Bot Stud

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BackgroundTartary buckwheat are very popular as an important functional food material and its cultivation is very widespread in our whole world, but there obviously lack works in the researches of genetic breeding for agricultural and medicinal utilization. The aim of this study is to obtain good germplasm resources for agricultural and medicinal use of tartary buckwheat (Fagopyrum tataricum) by inducing the tetraploid plants.ResultsFour cultivars of F. tataricum, that is, Qianwei 2#, Jinku 2#, Chuanqiao 1#, and Liuqiao 1# were selected to experiment. The tips of seedlings with two true leaves were treated by 0.25% (w/v) colchicine solution for 48, 72, and 96 h, respectively. The chromosome number of treated plants was determined by metaphase chromosome counting of root tip cells and PMCs (pollen mother cells) meiosis observation. Tetraploid induction successfully occurred in all three treatments with an efficiency ranging from 12.13 to 54.55%. The chromosome number of the diploid plants was 2n = 2x = 16, and that of the induced tetraploid plants was 2n = 4x = 32. The typical morphological and physiological qualities were compared between the control diploid and corresponding induced tetraploid plants. Results showed that the induced tetraploid plants had obviously larger leaves, flowers, and seeds. Moreover, the content of seed protein and flavonoid were also increased in the tetraploid plants. The pollen diameter and capsule size of diploid plants were significantly smaller than those of tetraploid plants.Conclusion Fagopyrum tataricum can be effectively induced into tetraploids by colchicines. The tetraploid induction can produce valuable germplasm resources for breeding and is a practicable breeding way in F. tataricum.

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Genetic diversity of four new species related to southwestern Sichuan buckwheats as revealed by karyotype, ISSR and allozyme characterization

Cited by 28 publications

References 28 publications

Classification and systematics of the <i>Fagopyrum</i> species

Classification and systematics of the <i>Fagopyrum</i> species

Fagopyrum esculentum ssp. ancestrale-A Hybrid Species Between Diploid F. cymosum and F. esculentum

Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Fagopyrum tataricum (L.) Gaertn

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