Genetic diversity and strain-typing in cultivated strains of Lentinula edodes (the shii-take mushroom) in Japan by AFLP analysis

Terashima, Kazuhisa; Matsumoto, Teruyuki; Hasebe, Kozaburd; Fukumasa-Nakai, Yukitaka

doi:10.1017/s0953756201005007

Cited by 37 publications

(37 citation statements)

References 17 publications

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“…The EcoRI primers were labeled with the fluorescent dye 6=-carboxyfluorescein to enable detection with an ABI Prism 3130 genetic analyzer (Applied Biosystems, LLC, Foster City, CA). Reaction conditions and the thermocycler program used for selective amplification were as described by Terashima et al (48,49). AFLP fragments were detected using the ABI Prism 3130 genetic analyzer, and their sizes were analyzed using Gene Mapper v. 4.0 software (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

Okuda

Ueda

Obatake³

et al. 2012

Appl Environ Microbiol

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A large number of spores from fruiting bodies can lead to allergic reactions and other problems during the cultivation of edible mushrooms, including Pleurotus eryngii (DC.) Quél. A cultivar harboring a sporulation-deficient (sporeless) mutation would be useful for preventing these problems, but traditional breeding requires extensive time and labor. In this study, using a sporeless P. eryngii strain, we constructed a genetic linkage map to introduce a molecular breeding program like marker-assisted selection. Based on the segregation of 294 amplified fragment length polymorphism markers, two mating type factors, and the sporeless trait, the linkage map consisted of 11 linkage groups with a total length of 837.2 centimorgans (cM). The gene region responsible for the sporeless trait was located in linkage group IX with 32 amplified fragment length polymorphism markers and the B mating type factor. We also identified eight markers closely linked (within 1.2 cM) to the sporeless locus using bulked-segregant analysis-based amplified fragment length polymorphism. One such amplified fragment length polymorphism marker was converted into two sequence-tagged site markers, SD488-I and SD488-II. Using 14 wild isolates, sequence-tagged site analysis indicated the potential usefulness of the combination of two sequence-tagged site markers in cross-breeding of the sporeless strain. It also suggested that a map constructed for P. eryngii has adequate accuracy for marker-assisted selection.

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Section: Methodsmentioning

confidence: 99%

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

Okuda

Ueda

Obatake³

et al. 2012

Appl Environ Microbiol

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“…In our experiment, all the 19 genotypes were cultivated under the same conditions with the fruiting temperature set at 13 15 C. The strain HmJ2632 was found to grow at a signifi cantly slower rate when compared to the others. Terashima et al (2002) reported that the cultivated L. edodes strains with the same fruiting season type shared the same gene pool. Zhang et al (2007) also observed a similar phenomenon in Chinese cultivated strains of L. edodes.…”

Section: Discussionmentioning

confidence: 99%

“…Developing and genotyping AFLP markers have been reported in several fungi (Boucias et al, 2000;Majer et al, 1996;Purwantara et al, 2000). The technique has also been applied to genotypic identifi cation in only a few edible mushrooms such as Lentinula edodes (Terashima and Matsumoto, 2004;Terashima et al, 2002) and Pleurotus eryngii (Urbanelli et al, 2007). However, to our best knowledge, there is no report that developed a reliable experimental protocol for genotyping H. marmoreus with AFLP.…”

Section: Introductionmentioning

confidence: 99%

Development of AFLP markers and phylogenetic analysis in Hypsizygus marmoreus

Wang

Feng

et al. 2009

J. Gen. Appl. Microbiol.

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The present study was designed to develop a reliable experimental protocol for detecting and genotyping amplifi ed fragment length polymorphism (AFLP) markers in the commercially important edible mushroom Hypsizygus marmoreus; and to use the markers to evaluate the genetic polymorphisms and phylogenetic relationships among 19 strains of H. marmoreus. Using a set of ten primer pairs, 609 AFLP markers were identifi ed, of which 532 were found segregating among 19 cultivated varieties and laboratory stock strains. An estimate of genetic relationships among the varieties/strains based on the AFLP markers was obtained by primary phylogenetic analysis using UPGMA. Our data demonstrate abundant genetic variation in the natural population of H. marmoreus and facilitate further analysis of genetic divergence within the species. The present study is useful for understanding the genetic structure of this species and uncovering genetic control of economically important traits for effi cient breeding programs.

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“…Meanwhile, Vos et al (1995) developed AFLP (amplified fragment length polymorphism) analysis based on selective PCR amplification of genomic DNA restriction fragments, and demonstrated that AFLP analysis could reproducibly detect a large number of polymorphic loci. Furthermore, we previously reported that AFLP analysis reproducibly identified more DNA markers than isozyme and RAPD profiling and that this technique could be used to differentiate major commercial strains of Japanese shiitake (Terashima et al 2002) using freeze-dried mycelia.…”

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confidence: 98%

Strain typing of shiitake (Lentinula edodes) cultivars by AFLP analysis, focusing on a heat-dried fruiting body

Terashima

Matsumoto

2004

Mycoscience

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To validate strain typing by amplified fragment length polymorphism (AFLP) analysis in shiitake (Lentinula edodes) cultivars, the reproducibility of AFLP markers with DNA extracted from the heat-dried fruiting body was evaluated. DNAs were extracted from three different portions of the heat-dried fruiting body -the stipe, pileus, and gill -and AFLP analysis of all parts was carried out using two combinations of selected amplification primer pairs. AFLP profiles of DNA from the gill tissue of heatdried fruiting body were almost identical to those of cultured mycelia in the same strains, although it was difficult to detect reproducible AFLP profiles from stipe and pileus DNA. These results indicated that AFLP analysis would be applicable for strain typing with heat-dried fruiting bodies of L. edodes by using the DNA extracted from gills.

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Genetic diversity and strain-typing in cultivated strains of Lentinula edodes (the shii-take mushroom) in Japan by AFLP analysis

Cited by 37 publications

References 17 publications

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

Development of AFLP markers and phylogenetic analysis in Hypsizygus marmoreus

Strain typing of shiitake (Lentinula edodes) cultivars by AFLP analysis, focusing on a heat-dried fruiting body

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