Genetic diversity and species-specific PCR-based markers from AFLP analyses of Thai bananas

Wongniam, Sirapope; Somana, Jamorn; Swangpol, Sasivimon; Seelanan, Tosak; Chareonsap, P.P.; Chadchawan, Supachitra; Jenjittikul, Thaya

doi:10.1016/j.bse.2010.03.015

Cited by 9 publications

(8 citation statements)

References 28 publications

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“…Genetic diversity of 16 accessions of M. balbisiana in India was 32% (Uma et al 2006) while genetic diversity of 19 Thailand accessions was 33% (Wongniam et al 2010). This result may support the hypothesis that the center of origin and center of diversity of M. balbisiana might came from South Asia and Indochina (de Langhe 2009;Aung et al 2010).…”

Section: Discussionsupporting

confidence: 60%

“…Genetic diversity of 21 accessions M. balbisiana from Indonesia was 13.8% which was much lower compared to genetic diversity of M. balbisiana from India (Uma et al 2006) and Thailand (Wongniam et al 2010). Genetic diversity of 16 accessions of M. balbisiana in India was 32% (Uma et al 2006) while genetic diversity of 19 Thailand accessions was 33% (Wongniam et al 2010).…”

Section: Discussionmentioning

confidence: 72%

“…The analysis was conducted by AFLP method (Vos et al 1995) with minor modification, i.e., without radioactive labeling of primer, but by fluourescence nucleic acid dye. Twenty six primer pairs for selective amplification were selected from previous studies (Ude et al 2002a;El-Khisin et al 2009;Opara et al 2010;Wongniam et al 2010). Restriction buffer, restriction enzyme, ligation buffer, adapter, pre-selective amplification primer, pre-selective amplification PCR reagent, and selective primers were the product of Invitrogen AFLP Analysis System I kit.…”

Section: Dna Extractionmentioning

confidence: 99%

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Genetic Diversity of Musa balbisiana Colla in Indonesia Based on AFLP Marker

Ahmad

Megia

Poerba

2014

HAYATI Journal of Biosciences

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Musa balbisianaColla, known as Pisang Klutuk/Pisang Batu, is important for banana breeding program due to its B genome and often considered to be resistant to pest, disease and drought. Banana is a big and tall herb up to 7-9 m in height.The study was conducted to assess genetic diversity of 21 accessions of M. balbisiana cultivars/ varieties from Indonesia based on Amplified Fragment Length Polymorphism (AFLP) markers in order to provide basic information to support banana breeding program. Analyses of similarity index of Nei and Li and clustering based on UPGMA was conducted using NTSYS program. Principal Component Analysis (PCA) was conducted by MINITAB 14. Twenty two primer pairs of AFLP markers produced 485 AFLP bands varying from 51-3206 bp in size and 46.18% of the AFLP bands were polymorphic. Genetic diversity among the 21 accessions of M. balbisiana was 13.8%. The genetic diversity of wild M. balbisiana was 12.9%, higher than the genetic diversity of cultivated M. balbisiana which was 11.5%. Cluster analysis based on UPGMA suggested that wild M. balbisiana and cultivated M. balbisiana could not be separated into different clusters. Both cluster analysis and PCA produced the same three groups of the accessions. The PCA analyses showed that 17 AFLP bands were responsible for the grouping.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 72%

Section: Dna Extractionmentioning

confidence: 99%

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Genetic Diversity of Musa balbisiana Colla in Indonesia Based on AFLP Marker

Ahmad

Megia

Poerba

2014

HAYATI Journal of Biosciences

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“…The passport data to source locations is presented in Online Resource 1. Native species were collected from wild habitats with specimens conservation methods as described in Wongniam et al (2010) including recently reported species, Musa yunnanensis (SS&JS 235, Häkkinen and Hong 2007) and M. serpentina (SS&JS 246, Swangpol and Somana 2011) and introduced species, M. ornata (SS&JS 433), M. coccinea (SS&JS 223), and Musella lasiocarpa (SS&JS 231), were ornamental plants and maintained in institute or private collections. Cultivars samples and the given names called by the local growers were obtained from several banana distributors.…”

Section: Methodsmentioning

confidence: 99%

“…SSR, as the DNA marker, consists of tandem repeats of short nucleotides motif which allocating throughout the genome of eukaryotes (Weising et al 2005). The polymorphisms obtained from SSR are highly informative with codominant inheritance.…”

Section: Introductionmentioning

confidence: 99%

Development of SSR markers from Musa balbisiana for genetic diversity analysis among Thai bananas

et al. 2016

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Bananas in Thailand have been surveyed by our team to be at least 140 cultivars in the plantations, 10 wild species and, 4 introduced species. To characterize the genetic relationship of species and cultivars, a set of novel SSR markers was developed. Totaling 53 clones containing SSR motifs were isolated from SSR-enriched library of wild Musa balbisiana Colla 'Tani' (BB). Selected positive clones were used to design 28 primer pairs for amplification of 12 wild and 82 cultivar accessions with genome designations AA, AB, AAA, AAB, ABB, and BBB. These SSR markers loci were homology searched to the banana genomes to map their locations. The seven-sets multiplex PCR approach using four fluorescent-labeled universal primers were utilized for cost effectiveness. Capillary fragment analysis yielded the accurate size of amplicons for evaluation of particular patterns for each cultivar. Phylogram and Structure analysis presented the specific genotype of genome groups (A and B genotypes, polyploid hybrid genomes) and cultivar groups. By A:B specific alleles ratio, accurate genome designations of hybrids can be determined. Additionally, a marker, characterized to be partial plastid ycf2 gene, indicated the maternal identification of hybrid cultivars. One SSR marker was also preliminary tested with some wild species and advised to be the candidate fingerprinting marker for species identification. In conclusion, SSR marker sets developed here proved their exploitation in detailed identity and relationship of cultivated bananas, which would be useful for genetic conservation and ongoing breeding programs in Thailand and other areas.

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Genes and Markers: Application in Banana Crop Improvement

Biswas

2016

Banana: Genomics and Transgenic Approaches for Genetic Improvement

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Genetic diversity and species-specific PCR-based markers from AFLP analyses of Thai bananas

Cited by 9 publications

References 28 publications

Genetic Diversity of Musa balbisiana Colla in Indonesia Based on AFLP Marker

Genetic Diversity of Musa balbisiana Colla in Indonesia Based on AFLP Marker

Development of SSR markers from Musa balbisiana for genetic diversity analysis among Thai bananas

Genes and Markers: Application in Banana Crop Improvement

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