Genetic diversity and relationships detected by isozyme and RAPD analysis of crop and wild species of Amaranthus

Using of Inter Microsatellite Polymorphism to evaluate gamma-irradiated<br>Amaranth mutants

“…Molecular markers approach was successfully used to understand intraand inter-specific genetic diversity and/or evolutionary relationships in Amaranths (Lanoue et al, 1996;Chan and Sun, 1997;Sun et al, 1999;Xu et al, 2001;Lee et al, 2008, Tony-Odigie et al, 2012Mladenovic et al, 2012). Lee et al (2008) reported the potential of microsatellite based markers for the A. hypochondriacus assessment; when they successfully amplified in all the tested species 12 loci and demonstrated the applicability of these markers for the study not only of intra-, but for interspecific genetic diversity of amaranth, too.…”

“…Along with the RAPD technique, both of the still preferred method for fast initiating identification of genotypes as they are relatively inexpensive, utilizes arbitrary primers, and randomly samples a potentially large number of loci in a less complex pattern than other PCR based markers (Milella et al, 2005(Milella et al, , 2011. Transue et al (1994) and Chan and Sun (1997) used RAPD markers for the study of evolutionary relationships among grain amaranths and their wild relatives. Popa et al (2010) studied genetic diversity and phylogenetic relationship among six species of Amaranthus by RAPD markers and showed that there is slightly intra and inter species polymorphism.…”

“…This work was based on isozyme polymorphisms and several authors have since expanded molecular diversity studies in the genus. In a study including both isozyme and random amplified polymorphic DNA (RAPD) markers, Chan and Sun (1997) reported molecular phylogenies of cultivated and wild amaranths. Authors evaluated 23 different species, including the three cultivated for grain as well as accessions of all species in the A. hybridus aggregate.…”

Analysis of the relationships between species of the genus Prosopis revealed by the use of molecular markers

“…There is a plethora of molecular markers based on the polymerase chain reaction (PCR) and over the last 10 years a large body of information about them has accumulated [15,42]. These markers have been used in wild plants to uncover genetic diversity and relatedness in plant populations, to measure the influence of breeding systems on diversity, and in plant taxonomy [4,9,10,17,20]. With cultivated plants they have been used in cultivar identification, to determine genetic relatedness among cultivars, for paternity testing, in the identification of hybrids and in genome mapping projects [1-3, 19, 21, 25, Burkart [7,8].…”

Methodological Approaches to Simple Enzyme Polymorphism Analyses of Amaranth Species (Amaranthus SP.)

“…The low allozyme variation in total 52 amaranth populations and two cultivars were described by Yudina et al (2005). The more accurate estimates of genetic diversity and of relationships within and among crop species and wild relatives of amaranth were generated by means of complementary approach using information from both isozymes and RAPD analyses (Chan & Sun 1997). On the contrary, a high degree of polymorphism was found within and between populations of amaranth even in neighbouring populations, as characterized by different alleles or isoenzymes (Jacobsen & Mujica 2003).…”