Genetic diversity and relationship between Bradyrhizobium strains isolated from blackgram and cowpea

Saleena, Lilly M.; Ponnusamy, Loganathan; Rangarajan, Sunita; Nair, Sudha

doi:10.1007/s003740100391

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…In Zanzibar soils, salinity level is high and that is why most of these isolates could grow at high concentration. The bacterial strains that survive in saline environment can successfully colonize plants grown under the same condition (SALEENA et al 2001). Our results also confirm that the natural habitat of the strains performs a selection pressure for tolerance to salinity.…”

Section: Discussionsupporting

confidence: 76%

Isolation, characterization and beneficial effects of rice associated plant growth promoting bacteria from Zanzibar soils

Yasmin

Bakar

Malik

et al. 2004

J. Basic Microbiol.

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This study was undertaken to isolate and characterize plant growth promoting bacteria (PGPB) occurring in four soils of Zanzibar, Tanzania as well as to evaluate their potential use as biofertilizers for rice. A total of 12 PGPB strains were isolated from rice and studied for growth characteristics, carbon/nitrogen source utilization patterns using QTS-24 kits, phosphate solubilization, indole acetic acid (IAA) production, antibiotic resistance patterns and growth at different pH, temperature and salt concentrations. All the isolates were motile and gram negative except Z3-4. Acetylene reduction activity was detected in all isolates ranging from 5.9-76.4 nmole C2H2 reduced/h x mg protein while 9 isolates produced IAA ranged from 20-90.8 mg/l. Most of the isolates showed resistance against different environmental stresses like 10-40 degrees C temperature, 0.2-1 M salt concentration and 4-8.5 pH range. Only one isolate Z2-7 formed clear zones on Pikovskaia's medium showing its ability to solubilize phosphates. Z3-2 was used to develop fluorescent antibodies to check the cross reactivity of the isolates. Inoculation of these bacterial isolates resulted in higher plant biomass, root area, and total N and P contents on Tanzanian rice variety BKN PRAT3036B under controlled conditions. Bacillus sp. Z3-4 and Azospirillum sp. Z3-1 are effective strains and, after further testing under field conditions, can be used for inoculum production of rice in Tanzania. The plant growth promoting effects of these PGPRs suggest that these can be exploited to improve crop productivity of rice in Tanzania.

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Section: Discussionsupporting

confidence: 76%

Isolation, characterization and beneficial effects of rice associated plant growth promoting bacteria from Zanzibar soils

Yasmin

Bakar

Malik

et al. 2004

J. Basic Microbiol.

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“…It may be correlated with previous data that showed that it is more effective at promoting growth of wheat and had a moderate effect on rice . The genetic diversity estimates were higher between PGPB strains recovered from different regions than between PGPB isolates from different hosts, confirming the reports of Gomes et al (2001) and Saleena et al (2001). RAPD profiles resolved differences on a much finer scale between isolates.…”

Section: Random Amplified Polymorphic Dna (Rapd) Analysissupporting

confidence: 85%

Plant growth-promoting bacteria as biofertilizer

Hafeez

Yasmin

Ariani

et al. 2006

Agron. Sustain. Dev.

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Seventeen rhizobacteria isolated from different ecological regions, i.e. Brazil, Indonesia, Mongolia and Pakistan were studied to develop inoculants for wheat, maize and rice. Almost all the bacterial isolates were Gram-negative, fast-growing motile rods and utilized a wide range of carbon sources. These isolates produced indole-3-acetic acid at concentrations ranging from 0.8-42.1 µg/mL, irrespective of the region. Fifteen isolates fixed N at rates ranging from 20.3-556.8 nmole C 2 H 2 reduced/h/vial. Isolate 8N-4 from Mongolia produced the highest amount of indole-3-acetic acid (42.1 µg/mL), produced siderophores (0.3 mg/mL) and was the only isolate that solubilized phosphate (188.7 µg P/mL). Inoculation of the wheat variety Orkhon with 8N-4 isolate resulted in the maximum increase in plant biomass, root length, and total N and P contents in plants. Random amplified polymorphic deoxyribonucleic acid (RAPD) analysis, conducted with 60 decamer primers, revealed a high level of polymorphism among the bacterial isolates from different geographic regions and a low level of polymorphism among isolates from the same region. The complete 16S rRNA gene sequence analysis demonstrated that 8N-4 is a Bacillus pumilus strain (Accession number AY548949). It was concluded that Bacillus pumilus 8N-4 can be used as a bio-inoculant for biofertilizer production to increase the crop yield of wheat variety Orkhon in Mongolia. Rhizobacteria / genetic diversity / RAPD

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“…The isolates that showed rapid growth and, more generally, with acid reaction in culture medium obtained in areas of cerrado suggest greater adaptability to this condition ( Table 2). The bacteria that nodulate cowpea have been routinely considered as belonging to the miscellaneous group "cowpea" or Bradyrhizobium spp., comprising a large number of slow-growing strains capable of nodulating several species of herbaceous legumes common in the tropical region (Saleena et al, 2001;Zilli et al, 2006). In this study, approximately 55% of isolates obtained from different regions collection showed rapid growth in culture medium indicating that the limit strain capable of Cowpea nodular goes beyond the group's Bradyrhizobium spp (Tables 2 and 3).…”

Section: Resultsmentioning

confidence: 65%

Isolation and phenotypic characterization of rhizobia that nodulate cowpea in the Cerrado in Tocantins State, Brazil

Júnior

Oliveira²,

Castro³

et al. 2013

J. Biotechnol. Biodivers.

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Depending on the nutritional characteristics and hardiness, the cowpea has become an important source of protein in North and Northeast regions of Brazil. The cowpea benefits from biological nitrogen fixation (BNF) and may receive part of the nitrogen needed for culture via symbiosis, which reduces production costs. Aiming to contribute to the optimization of the BNF in the culture of cowpea (Vigna unguiculata (L) Walp) in the cerrado of Tocantins, through the effectiveness of populations and diversity of rhizobia obtained in seven areas with and without crops , phenotypic isolation and characterization were performed (pH, time of growth characteristics of the colonies and mucus). We obtained 72 rhizobia and evaluated in a dendrogram showed a great diversity, with the formation of 18 groups and the five large groups with 70% similarity. The study of morphological and physiological characteristics reveals a fairly wide diversity of rhizobia and is usually related to studies at the DNA level.

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Genetic diversity and relationship between Bradyrhizobium strains isolated from blackgram and cowpea

Cited by 12 publications

References 12 publications

Isolation, characterization and beneficial effects of rice associated plant growth promoting bacteria from Zanzibar soils

Isolation, characterization and beneficial effects of rice associated plant growth promoting bacteria from Zanzibar soils

Plant growth-promoting bacteria as biofertilizer

Isolation and phenotypic characterization of rhizobia that nodulate cowpea in the Cerrado in Tocantins State, Brazil

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