Genetic diversity and relationship among faba bean (Vicia faba L.) germplasm entries as revealed by TRAP markers

Abstract

Target region amplification polymorphism markers were used to assess the genetic diversity and relationship among 151 worldwide collected faba bean (Vicia faba L.) entries (137 accessions maintained at the USDA–ARS, Pullman, WA, 2 commercial varieties and 12 elite cultivars and advanced breeding lines obtained from Link of Georg-August University, Germany). Twelve primer combinations (six sets of polymerase chain reaction) amplified a total of 221 markers, of which 122 (55.2%) were polymorphic and could discriminate all the 151 entries. A high level of polymorphism was revealed among the accessions with an estimated average pairwise similarity of 63.2%, ranging from 36.9 to 90.2%. Cluster analysis divided the 151 accessions into five major groups with 2–101 entries each and revealed a substantial association between the molecular diversity and the geographic origin. All 101 accessions in Group V are originated from China and 13 of the 15 accessions in Group II were from Afghanistan. Thirty-two individual plants were sampled from two entries to assess the intra-accession variation. It was found that the advanced inbred line (Hiverna/5-EP1) had very little variation (5.0%), while the original collection (PI 577746) possessed a very high amount of variation (47.1%). This is consistent with the previous reports that faba bean landraces have a high level of outcrossing in production fields and thus contain larger amount variation within each landrace. One implication of this observation for germplasm management is that a relatively larger population is needed in regeneration to mitigate the possible loss of genetic variation due to genetic drift.

Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers

“…A large amount of variation within faba bean landraces was also demonstrated using Target Region Amplification Polymorphism (TRAP) [51], probably as a result of high levels of outcrossing as previously suggested [47,48,[50][51][52].…”

“…The genetic variability detected within the faba bean accessions studied was also reported for Greek, Sicilian and Tunisian germplasm using Inter-Simple Sequence Repeat (ISSR), Amplified Fragment Length Polymorphism (AFLP) and Sequence Specific Amplified Polymorphism (SSAP) markers [48][49][50]. A large amount of variation within faba bean landraces was also demonstrated using Target Region Amplification Polymorphism (TRAP) [51], probably as a result of high levels of outcrossing as previously suggested [47,48,[50][51][52].…”

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

“…Repetitive sequences and other DNA-based markers have been used to assess genetic diversity (Zeid et al, 2003;Gutierrez et al, 2006;Torres et al, 2006;Sanz et al, 2007;Terzopolous and Bebeli, 2008;Zong et al, 2009;Kwon et al, 2010) among cultivated faba genotypes. Phenotypic traits of agronomic interest were used to evaluate ex situ faba bean germplasm collections in Europe, at ICARDA and in China (reviewed in Duc et al, 2010).…”

Genetic diversity of Palestine landraces of faba bean (Vicia faba) based on RAPD markers

“…The possible explanation for that is Palestine in general and the West Bank in particular "in which all landraces were collected" is a small geographical area, and therefore, we would expect a narrow genetic diversity of faba bean landraces. In addition, most of these landraces may have moved from one place to another through human activities (Kwon et al, 2010). However, only VF-22, VF-23, and VF-24 landraces, which were collected from the Bethlehem region were clustered together.…”