Genetic diversity and relatedness among seven red deer (Cervus elaphus) populations

Maršálková, Lenka; Židek, Radoslav; Pokorádi, Jaroslav; Golian, Jozef; Belej, Ľubomí­r

doi:10.5219/320

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…Generally, good level of observed heterozygosity was found across all species. In the Slovak Red deer population the rate of heterozygous was ascertained at 41% and it showed lower level of heterozygosity that observed in free living Red deer Ernst et al (2011) [12] and Maršálková et al (2014) [13] using microsatellite markers. Even if the trend of heterozygosity had a declining tendency, the obtained value of F IS (0.04) can be still regarded as suffi cient for the maintaining of genetic diversity within them.…”

Section: Discussionmentioning

confidence: 92%

“…Due to the hybridisation with the Japanese Sika deer and North American Wapiti [9][10][11]. For long-term conservation and development purpose, it therefore appears compulsory to manage their wildlife to maintain both species survival and within species genetic diversity [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…The phylogenic relationships and genetic diversity, as well as the level of admixture across native populations of the Cervidae family have been investigated primarily based on mitochondrial DNA [14,15] and microsatellite markers [11][12][13]. Profi ling a large number of single nucleotide polymorphisms (SNPs) was only realistically possible for organisms with well-developmed genomic resources, and the high cost of developing these resources has been a major impediment to studies for non-model organisms, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Genomic variation across cervid species in respect to the estimation of red deer diversity

et al. 2017

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The aim of this study was to assess the genetic variations and relationships across evolutionary related cervid species in order to estimate the genetic diversity of the Red deer population that inhabits the forest area in the south-western part of Slovakia. The study was based on the application of cross-species SNP genotyping. The genomic data were obtained from a total of 86 individuals representing six genera (Axis, Dama, Cervus, Alces, Rangifer, and Odocoileus) using Illumina BovineSNP50 BeadChip. From 38.85% of successfully genotyped loci up to 1,532 SNPs showed polymorphism and were informative for subsequent analyses of the diversity and interspecifi c genetic relationships. Generally, a good level of observed heterozygosity was found across all species. The value of F IS (0.23±0.13) signalised the increase of a homozygous proportion within them. The application of molecular variance analysis to the hierarchical population structure showed that most of the variation was conserved within separate species (96%). The performed diversity analysis of Slovak Red deer population and comparative analysis of their phylogenic relationships among subspecies from genus Cervus did not identify a remarkable loss of genetic variability. Also, were not identifi ed any degree of admixture that could be due to the historical background of deer farming in Slovakia or reintroduction and hybridisation by other species from genus Cervus (C.canadensis, and C. nippon) which are the major risk of loss of autochthonous Red deer populations in many areas of Central Europe. The analysis of individual's ancestry showed consistent results with patterns of evaluated group differentiations which means low migration rates among all species.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genomic variation across cervid species in respect to the estimation of red deer diversity

et al. 2017

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“…The prevalence of homozygotes in particular groups and on the level of whole population confirmed also the value of Wright's F IS (0.294±0.008) and F IT indexes (0.821±0.012). The results showed lower degree of genetic variability within Slovak red deer population than presented Maršálková et al (2014) using microsatellite markers. In addition, the value of F IS index can signalize the potential risk of inbreeding increase accompanied by the decrease of genetic diversity within population.…”

Section: Resultsmentioning

confidence: 53%

The Degree of Genetic Admixture Within Species From Genus Cervus

Moravčíková¹,

Kasarda²,

Kadlečík³

2017

AgricultForest

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SUMMARYThe aim of present study is to estimate the level of admixture within species originating from genus Cervus (family Cervidae) that can reflect not only their phylogeny origin, but mainly the impact of introgression and hybridization in Red deer population inhabit the forest area of Slovakia. It was happened over the last few centuries. Recently, the introduction and hybridization by non-native species is considered as the major risk of diversity loss within autochthonous Red deer populations in area of Central Europe. The genomic data were obtained from in total 61 animals (species A. axis, D. dama, C. elaphus, C. Nippon, C. canadensis) based on applying the cross-species SNP genotyping (Illumina BovineSNP50 BeadChip). The quality control of genotyping data has been performed to eliminate any SNPs with genotyping errors (loci with >10% missing genotypes), less informative markers (MAF<0.01) and markers deviating from Hardy-Weinberg equilibrium limit of 0.0001. From totally 43.21% of successfully genotyped markers up to 843 were informative for subsequent estimation. As expected the presence of admixed individuals was found within Red deer and Wapiti populations, but the signal of cross-species admixture was low. The membership probabilities of individuals were higher than 90% within each of analysed cervid group. Our study showed that the Slovak Red deer population can provide valuable gene pool within Central European deer populations in respect to the management of genetic resources conservation strategies.

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“…Molecular markers can provide an effective tool for efficcient selection of desired agronomic traits because they are based on the plant genotypes and thus, are independent of environmental variation. Nowadays, several molecular markers are developed, of which simple sequence repeats (SSRs) or microsatellites are the most widely used types (Jenabi et al, 2011;Maršálková et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of rye cultivars

Balážová

Petrovičová

Gálová

et al. 2016

Potr. S. J. F. Sci.

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The results of molecular analysis of 45 rye taxa (Secale cereale L.) represented by agricultural varieties originated from Central Europe and the Union of Soviet Socialist Republics (SUN) are presented. The genetic diversity of rye cultivars by 6 SSR markers was evaluated. Six specific microsatellite primer pairs produced 58 polymorphic alleles with an average of 9.7 alleles per locus. The number of alleles ranged from 6 (SCM2) to 14 (SCM86). Genetic polymorphism was characterized based on diversity index (DI), probability of identity (PI) and polymorphic information content (PIC). The diversity index (DI) of SSR markers ranged from 0.5478 (SCM2) to 0.887 (SCM86) with an average of 0.778. The lowest value of polymorphic information content was recorded for SCM2 (0.484) and the highest value for SCM86 (0.885) of PIC was detected in SCM86 with an average of 0.760.The dendrogram of genetic similarity was constructed, based on UPGMA algorithm. The hierarchical cluster analysis divided rye genotypes into 4 main clusters. The first cluster of 14 genotypes was subdivided in two subclusters (1a and 1b) where 50% of genotypes were Czechoslovak origin. The second cluster contained four genotypes were three (75%) of them had Czech or Czechoslovak origin. In the third subcluster separated three rye genotypes of different origin. The rest (24) of rye genotypes in the fourth cluster were divided into two subclusters (4a and 4b) where clearly separated group of Polish (4aa) and Czech and Czechoslovak (4ab) genotypes. Two genotypes of 4aa subcluster (Wojcieszyckie and Dankowskie Nowe) from Poland were genetically the closest. In the dendrogram alle genotypes were differentiated and clustering partially reflects geographic origin of studied rye genotypes. In this experiment, SSRs markers proved to be a high informative and usefull tool in genetic diversity research for the distinguishing and characterization of close related varieties.

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Genetic diversity and relatedness among seven red deer (Cervus elaphus) populations

Cited by 6 publications

References 21 publications

Genomic variation across cervid species in respect to the estimation of red deer diversity

Genomic variation across cervid species in respect to the estimation of red deer diversity

The Degree of Genetic Admixture Within Species From Genus Cervus

Molecular characterization of rye cultivars

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