Genetic diversity and population structure of a common bean (Phaseolus vulgaris L.) collection from Calabria (Italy)

SSR markers distinguish traditional Italian bean (Phaseolus vulgaris L.) landraces from Lamon

“…Therefore, these latter seven microsatellites proved to be the most discriminating and polymorphic markers for the rapid and unambiguous identification of all the samples analysed here. Such variability of SSR markers was previously reported also by Kwak and Gepts (2009) and Mercati et al (2013). It might be speculated that the reduced number of alleles we observed could be the consequence of several factors, such as the small sample set tested, the self-pollinating system and the genetic bottleneck of European bean varieties compared to the American gene pool (Angioi et al 2010).…”

“…DNA was isolated from seeds using the DNeasy plant mini kit (Qiagen, Valencia, USA). Based on the literature data, twelve SSRs were chosen among the most informative ones, namely: DROUGH1, AY1, AIA, PAX1, LIPOX, BETAG, BNG91-R, BNG91-R2 from Guerra-Sanz (2004); C42, C76, C130 from Wang et al (2012); BM160 from Mercati et al (2013). Molecular analyses were carried out using three biological replicates for each sample and PCR reactions were performed in triplicate, as described in Carputo et al (2013).…”

Morphological and genetic diversity among and within common bean (Phaseolus vulgaris L.) landraces from the Campania region (Southern Italy)

“…In those areas, common bean is probably the horticultural crop with the highest number of landraces (Perrino et al, 1984). The germplasm of different Italian regions has been characterized (Lioi et al, 2012;Mercati et al, 2013;Piergiovanni and Lioi, 2010;Raggi et al, 2013), but despite the long history of cultivation, the number of accessions (Hammer et al, 1989) and the presence of some well-known landraces that excel for product quality (e.g. "di Controne", "Dente di Morto"), the germplasm of the Campania region (Southern Italy) has been poorly investigated.…”

Genetic Diversity and Seed Quality of the “Badda” Common Bean from Sicily (Italy)

“…In the last decades, several landraces have been investigated to promote their safeguard, valorization, and on farm conservation [7][8][9]. Although the attention devoted by researchers to common bean landraces has greatly increased, studies on this topic are far from being exhaustive.…”

“…In particular, SSRs (Simple Sequence Repeats), or microsatellites, are short (mostly 2-4 base pair tandem repeats of DNA sequence; their polymorphism originates from a different number of repetitive core motifs present at one locus. They are useful genetic tools to construct genetic maps, to evaluate genetic diversity in commercial and local varieties, lines or genotypes of common bean [9,18,19].…”