SSR markers distinguish traditional Italian bean (Phaseolus vulgaris L.) landraces from Lamon

Abstract

Carucci F., Garramone R., Aversano R., Carputo D. (2017): SSR markers distinguish traditional Italian bean (Phaseolus vulgaris L.) landraces from Lamon. Czech J. Genet. Plant Breed., 53: 168−171.In this study, 12 microsatellite markers (SSR) were evaluated for their applicability to protect from frauds and misuse the Italian Protected Geographical Indication (PGI) product "Common bean from Lamon". SSR analysis generated polymorphic alleles, with an average of 4 alleles per locus and all in the range of molecular weight between 181 and 284 bp. Twenty-nine variety-specific fragments were identified, which might be reasonably adopted for characterization and traceability purposes. Cluster analysis well outlined the relationships between the genotypes studied. Overall, our study underlines the use and usefulness of molecular markers to protect both farmers and consumers from frauds.

Preserving Biodiversity in Marginal Rural Areas: Assessment of Morphological and Genetic Variability of a Sicilian Common Bean Germplasm Collection

“…The efficiency of each SSRs in genetic fingerprinting was rather high for all the loci (mean 0.8656) and BM160 appeared the most discriminant locus. The PIC value of locus BM160 showed similar values to those detected in previous Italian germplasm studies [48,49,58], by contrast, lower PIC values were observed in worldwide collection [51,59]. The discriminating power for each locus was comparable or higher than that reported for the same SSR locus (GATS91, BM210, BM160, PVBR25, BM172, PVBR163) in previous Italian collection studies [47][48][49].…”

“…In particular, six seed descriptors were analyzed: Coat pattern (SCP), color of coat darker (CSCD) and lighter (CSCL), prevalent color of coat (PCSC), shape (SSH) and brilliance (BS). Regarding the seed coat color, the term 'prevalent' was adopted by using 3 different states: (1) lighter color as background and darker color as stripes; (2) darker color as background and lighter color as stripes; (3) darker color and lighter color equally distributed [58]. Others six morpho-descriptors were added to the accession characterization: Color of flower banner (CFB) and wings (CFW), immature (IPC) and mature (MPC) pod color, pod curvature (PC) and leaf shape (LS).…”

Investigation of the Genetic Structure of Some Common Bean (Phaseolus vulgaris L.) Commercial Varieties and Genotypes Used as a Genitor with SSR and SNP markers

“…Pereira et al (2019) using the toucher method and evaluating 27 SSR loci, 17 bean genotypes formed 4 different groups in their clustering analysis. In the study of Carucci et al (2017), the genetic structure of Italian local bean cultivars was analysed with 12 SSR loci, the Ho value was calculated as 0.24.…”

“…It is possible to easily determine the genetic structure, create molecular maps, and label the characters of interest by PCR-based markers. Researchers have utilized various molecular markers to conduct molecular genetic studies in common bean populations/genotypes (Metais et al 2001(Metais et al , 2002Duran et al 2005;Sicard et al 2005;Angioi et al 2010;Buah et al 2017;Carucci et al 2017).…”