Genetic diversity and population structure of wild pear (<i>Pyrus pyraster</i> (L.) Burgsd.) in Poland

Wolko, Łukasz; Bocianowski, Jan; Antkowiak, Wojciech; Słomski, Ryszard

doi:10.1515/biol-2015-0003

Cited by 21 publications

(19 citation statements)

References 46 publications

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“…However, supplementary analysis with different accessions from these species is needed to support these observations. Identification of private alleles using different marker systems has been reported earlier in different Pyrus species [15,[33][34][35][36][37]. The average number of produced alleles in each cultivar was compared between two groups of Iranian and introduced cultivars.…”

Section: Ssr Transferability and Genetic Diversitymentioning

confidence: 99%

Assessment of genetic structure among different pear species (Pyrusspp.) using apple-derived SSR and evidence of duplications in the pear genome

Erfani‐Moghadam

Zarei

2018

Biotechnology & Biotechnological Equipment

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Simple sequence repeat (SSRs) markers derived from Malus were used to assess their transferability in the analysis of the genetic diversity and relationship of some Pyrus species. All studied microsatellite loci produced fragments and were polymorphic among the studied samples. Fiftyone allelic variants were detected at eight SSR loci, ranging from five (MS14h03, CH02b10 and CH03g06) to nine (CH02h11a), with a mean of 6.37 alleles per locus. A relatively high level of diversity was recorded among the studied accessions. The average of Shannon's index (I) was 1.04. The Dice genetic similarity coefficient ranged greatly and 'Khoj 1' (a member of P. communis) and 'Shinko' (a cultivar belonging to P. pyrifolia) had the lowest (0.21) values, while 'Chojuro' and 'Nijisseiki', two cultivars belonging to P. pyrifolia, had the highest genetic similarity (0.97) among the studied samples. Bayesian cluster analysis and principal coordinates analysis plot separated the P. pyrifolia samples from other species, and formed three distinct groups, indicating high genetic differences between P. pyrifolia and other studied pear species. Several private alleles were observed in some of the studied species that would be of great importance for species identification. Furthermore, in 23 samples, more than two alleles were observed in the CH04e03 locus, indicating presence of locus duplication in these samples. Our observations suggest that, for this locus, at least two homologous chromosomes or genomic regions may be presented in the genome of some pear samples.

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Section: Ssr Transferability and Genetic Diversitymentioning

confidence: 99%

Assessment of genetic structure among different pear species (Pyrusspp.) using apple-derived SSR and evidence of duplications in the pear genome

Erfani‐Moghadam

Zarei

2018

Biotechnology & Biotechnological Equipment

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“…The resulting similarity was subjected to clustering by applying the unweighted pair group method using the arithmetic means algorithm. Variability for each primer combination was measured using the polymorphism information content, PIC (Wolko et al, 2015): Variability for each primer combination was measured using the polymorphism information content, PIC (Wolko et al, 2015):…”

Section: Data Scoring and Analysismentioning

confidence: 99%

“…All calculations were performed using GenAIEx 6.5 genetic analysis in Excel (Peakall and Smouse, 2012) and STATISTICA v.10 software (StatSoft, Inc.). Variability for each primer combination was measured using the polymorphism information content, PIC (Wolko et al, 2015):…”

Section: Data Scoring and Analysismentioning

confidence: 99%

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Liersch

Bocianowski

Wos³

et al. 2016

Crop Science

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The level of genetic diversity or similarity between parents has been proposed as a predictor of F1 generation performance and heterosis for seed yield. The objective of this study was to determine the feasibility of using amplified fragment length polymorphism (AFLP) fluorescent‐labeled primers to evaluate the genetic relationships between 101 oilseed rape (Brassica napus L.) genotypes to maximize the selection of different parents in a breeding program. The collection of genotypes included 25 cytoplasmic male sterility ogura and 21 restorer lines (Rfo). The set of genotypes was completed with B. napus cultivars of Polish, European, and Asian origin and resynthesized lines. Ten primer‐enzyme combinations (EcoRI and MseI) were used. Genetic similarities among genotypes were estimated in accordance with the Nei and Li coefficient of similarity, and then the unweighted pair group method using arithmetic averages method was applied for the creation of a dendrogram. A total of 344 reproducible, polymorphic markers were obtained. Unweighted pair group method using arithmetic averages clustering based on the Nei and Li coefficient of similarity separated the 101 genotypes into four distinct clusters. Analysis of molecular variance showed that 90% of the detected variation was found within the group, while the variation among groups contributed to only 10%. Our results indicate that the AFLP technique seemed to be a suitable molecular method for assessing genetic relationships between oilseed rape inbred lines with high levels of accuracy. Genetic similarity evaluated on the basis of AFLP molecular markers is a significant addition to classical methods of selection of parental components of winter oilseed rape hybrid varieties (e.g., ‘Poznaniak’).

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“…However, the easily cross-pollinations and the ambiguous taxonomic status of the obtained crosses make the determination of the exact number of Pyrus species relatively difficult. The presence of such huge number of species, sub-species, cultivars, hybrids, and clones make the application of reliable molecular tools crucial for their accurate identification, genetic characterization, and verification (9).…”

Section: Introducctionmentioning

confidence: 99%

Evaluation of genetic variations and phylogeny of the most popular pear (Pyrus communis L.) cultivars in Duhok city using AFLP markers

Ali

Othman²,

ZIBARI

et al. 2018

Innovaciencia

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Introduction: Genotyping and evaluation of genetic variation and polymorphic information content of the locally cultivated pear (Pyrus communis L.) might play an important role in building the genetic bank. These are also immensely important for present and future pear breeding program in the region. Methods: In the current study, AFLP markers have been employed to estimate the level of genetic diversity and to assess the phylogeny among the seven most popular pear cultivars in Duhok city. Results: Eight selective primer combinations generated a total of 653 AFLP fragments from which 445 (68.2%) fragments were polymorphic. The number of visible amplified products per primer combination were varied and ranged from 66 to 96 bands. The highest percentage of polymorphism (78.4%) was observed by the primer pair P174/M182, while the lowest percentage of polymorphism (58.6%) was observed by the primer pair P174/M100. The highest PIC (0.85) was obtained with the primer combination P174/ M182, while, the lowest PIC (0.49) was obtained by the primer combination P174/M307. The genetic distance was ranged from 0.1348 (between Danimarki and Amreki cultivars) to 0.3131 (between Italy and Zaafaran2 cultivars). Based on the AFLP data, all the seven pear genotypes were successfully clustered into two separate clusters (C1 and C2) with an out-group of Itali cultivar. Conclusions: Overall, it can be concluded that there was high polymorphism among the studied genotypes. Also, it can be stated that the AFLP was a reliable and a powerful technique in genotyping and discriminating of respective pear cultivars.Evaluación de variantes genéticas y filogenia de la pera común (Pyrus communis L.) cultivada en la ciudad Duhok empleando AFLP como marcadores moleculares Check for updatesHow to cite this paper: Ali S H., Othman H E., Zibari P A A., Jubrael J M S. Evaluation of genetic variations and phylogeny of the most popular pear (Pyrus communis L.) cultivars in Duhok city using AFLP markers, Duhok, Iraq. Innovaciencia. 2018; 6(1): 1-10.

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Genetic diversity and population structure of wild pear (Pyrus pyraster (L.) Burgsd.) in Poland

Cited by 21 publications

References 46 publications

Assessment of genetic structure among different pear species (Pyrusspp.) using apple-derived SSR and evidence of duplications in the pear genome

Assessment of genetic structure among different pear species (Pyrusspp.) using apple-derived SSR and evidence of duplications in the pear genome

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Evaluation of genetic variations and phylogeny of the most popular pear (Pyrus communis L.) cultivars in Duhok city using AFLP markers

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