Genetic diversity and population structure of wild and domesticated black tiger shrimp (Penaeus monodon) broodstocks in the Indo-Pacific regions using consolidated mtDNA and microsatellite markers

IOP Conf. Ser.: Earth Environ. Sci.

Tenriulo

Suryati

et al. 2022

The morphological difference of tiger shrimp, Penaeus monodon from Aceh waters is limited to explore for female and male discrimination using morphometric analyses. The study aimed to evaluate the morphological characters between female and male tiger shrimp using traditional and truss measurements. A total of 57 samples (39 females and 18 males) were analysed using 9 characters for traditional morphometric and 40 characters for truss morphometric. Multivariate analyses were applied to reveal the morphometric variation between male and female populations. The result of principal component analysis indicated that 4 characters, i.e: carapace length (CL), carapace width (CW), rostrum length (RL), and sixth segment length (SL6), were significantly different (P<0.05), while three truss morphological characters, i.e: distance between anterior and posterior carapace, distance between ventral posterior sixth segment to telson end, and distance between anterior and posterior second segment, were significantly discriminated (P<0.05) between female and male of tiger shrimp. Overall, 94.9% of the male and 88.9% of the female individuals were assigned to their native populations using traditional morphometric analysis, while truss morphometric was 87.2% and 88.9%, respectively. The individual proportion of 93.0% was properly classified into their original population group for traditional morphometric and 87.7% for truss morphometric characters.

Section: Truss Morphometricmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological discrimination of tiger shrimp Penaeus monodon between female and male based on traditional and truss morphometric analyses

IOP Conf. Ser.: Earth Environ. Sci.

Tenriulo

Suryati

et al. 2022

“…The increasing demand of the world market requires an increase in tiger shrimp production. To support the sustainable production of tiger shrimp, it needs quality broodstock with enough quantities for seed production (Wong et al, 2021). Until now, the provision of tiger shrimp broodstock for breeding still relies on mature wild broodstock.…”

Section: Introductionmentioning

confidence: 99%

Improved reproductive performance of captive male broodstock of tiger shrimp Penaeus monodon with 17α-methyltestosterone induction

Rosmiati

Israeli Journal of Aquaculture - Bamidgeh

Suryati

et al. 2022

The purpose of this study was to evaluate the influence of different 17αmethyltestosterone injection frequencies at optimal doses to improve the quality of spermatophore and reproductive performance of captive male broodstock of tiger shrimp Penaeus monodon. Broodstocks of tiger shrimp weighing 51-65 g for males and 86-161 g for females were collected from ponds and stored in a 3,000 m 3 concrete tank. Male broodstocks were injected with 17α-methyltestosterone at the optimized protocol at 300 ng/100 g body weight at different injection frequencies, three times, four times, and five times at seven-day intervals. As a control, male broodstocks were injected with a saline solution to determine the spermatophore quality. Evaluation of the reproductive performance of the captive male was observed in the captive female broodstock tiger shrimp using artificial insemination. The weight of spermatophore (0.041 g), gonadosomatic index (7.71%), the total number of spermatozoa (64 × 10 6 cells/mL) significantly increased, and the percentage of abnormal spermatozoa significantly decreased by 73% as well as hatching rate increased to 43.14% at the injection frequency of four times compared to the other treatments. In conclusion, injection of 17α-methyltestosterone at the dosage of 300 ng/100 g body weight at the frequency of giving four times was effective enough to improve the reproductive performance of captive tiger shrimp. Therefore, the method proposed can be a strategy to produce better larvae and increase the income of the aquaculture business.

“…Tiger shrimp, Penaeus monodon has been commercially cultivated in the Western Indo-Pacific region to meet the growing demand of the global seafood market. Penaeus monodon species have been cultivated extensively in various areas of their natural habitat where wild broodstock was originally sought for domestication programs [2]. The wild tiger shrimp 1119 (2022) 012038 IOP Publishing doi:10.1088/1755-1315/1119/1/012038 2 broodstock is seasonal [3] and the use of the wild broodstock for continuous seed production will lead to overexploitation.…”

Section: Introductionmentioning

confidence: 99%

Application of 17α-methyltestosterone to enhance broodstock maturation of the domesticated black tiger shrimp (Penaeus monodon): A comparative study of injection method and oral method

Rosmiati

Lante

IOP Conf. Ser.: Earth Environ. Sci.

et al. 2022

The effects of 17α-methyltestosterone administration on spermatophore quality in captive broodstock of tiger shrimp Penaeus monodon were investigated using injection method and oral method based on the optimized protocol. The experiment was designed to compare the oral administration group and injection group with two replications during a 38-day experimental period. Captive male broodstock of tiger shrimp weighing 57-95 g was collected from ponds and stored in a 10,000 m3 concrete tank (20 shrimp/tank). Male broodstocks were injected and orally administrated with 17α-methyltestosterone at the dose of 300 ng/100 g body weight. Data indicates that the injection of 17α-methyltestosterone showed the percentage of mature broodstock count and the percentage of normal spermatozoa count 1.07 and 1.38% higher and significantly differs from oral administration through mixing in feed at the maturation stage. However, at the time of the maturation and 1st re-maturation of both the methods, the weight of the spermatophores and the number of spermatozoa did not show any difference. Based on the comparison of the two methods used, the results are obtained that the administration of the hormone 17α-methyltestosterone can be done both through mixing in feed and through injection, but the administration of hormones through injection gives better results than the broodstock matures first simultaneously.