Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan

Lin, Mei-Wei; Chan, Hsiu-Chuan; Chi, Hsin; Chiu, Shu-Chun; Nora-Krūkle, Zaiga; Rasa-Dzelzkalēja, Santa; Vilmane, Anda; Murovska, Modra; Lin, Jer-Huei; Liu, Hsin‐Fu

doi:10.3390/ijerph17031086

Cited by 12 publications

(15 citation statements)

References 28 publications

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“…In the present study, the prevalence of HBoV in children suffering from AGE was 58% which is higher than the previous reports of AGE associated with HBoV in Egypt (2%) [13], Brazil (24 and 42%) [35], and Taiwan (8.5%) [36]. This difference in detection rate could be attributed to the difference of sensitivity of the detection method, geographical region, hygiene and sanitary conditions, and/or the sample size of the study.…”

Section: Discussioncontrasting

confidence: 83%

Etiology of diarrheal disease among children under 5 years in Egypt: a high incidence of human bocavirus

Rizk

Abd-Elmaksoud

Farid

et al. 2021

J. Egypt. Public. Health. Assoc.

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Background Human bocavirus (HBoV) is globally distributed and associated with respiratory and enteric infections. Limited data are available about the incidence of HBoV in Egyptian children. We aimed to investigate the association of HBoV genotypes in children with diarrheal disease and also to determine the possibility of HBoV co-infections with other human enteric pathogens. Methods A total of 102 stool samples were collected from children under five years old with diarrhea. Samples were analyzed for the presence of HBoV by real-time PCR. HBoV positive samples were tested for adenovirus (AdV), rotavirus (RoV), parasitic helminths, and enteric protozoa. Results HBoV was detected in 58% of examined cases. HBoV-3 was the most prevalent genotype observed (44%; 45 of 102), followed by HBoV-2/4 (33%; 34 of 102) and HBoV-1 (30%; 31 of 102). Although the incidence of HBoV was higher in males (66.6%; 34 of 51) than females (49%; 25 of 51), the analysis showed no significant difference for HBoV between genders. The average HBoV concentrations were 5.3 × 104 GC/g in males and 1.03 × 105 GC/g in females. Among the HBoV-positive samples, the single infection of HBoV was 52.5% (31/59), while the co-infections with multiple viruses were found in 1.7% (1/59) for HBoV and AdV, 33.9% (20/59) for HBoV and RoV, and 11.9% (7/59) for HBoV, and RoV and AdV. No co-infection with parasitic helminths or enteric protozoa was found. Conclusions The single infection of HBoV in some children suffering from acute gastroenteritis indicated that HBoV could be the main etiologic agent of the disease. The study highlights the high incidence of HBoVs genotypes with remarkable multiple co-infections in the pre-school children in Egypt.

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Section: Discussioncontrasting

confidence: 83%

Etiology of diarrheal disease among children under 5 years in Egypt: a high incidence of human bocavirus

Rizk

Abd-Elmaksoud

Farid

et al. 2021

J. Egypt. Public. Health. Assoc.

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“…The rate of human Bocavirus according to the result of conventional PCR was 9% (9/100 cases). This percentage is close to that of its prevalence in many countries, such as in Iran [13],Taiwan [14], Turkey [15] and Albania [16], where the rates were (8%,8.5%, 8.7%, 9.1% respectively), while this percentage was higher than in other countries such as Russia [17], Egypt [18], South Africa [19], India [20], Bangladesh [21] and Beijing-China [22]. This wide variation among studies could be attributed to the time and period of samples collection, number of samples, the age of the study population, the season, the techniques are used to diagnose the virus, variations in habits, geographical differences, climate changes and the immunological and nutritional situations of individuals, all of these factors may contribute to the difference in the prevalence rates of the virus between studies.…”

Section: Discussionsupporting

confidence: 63%

Detecting and Genotyping of Human Bocavirus among Children with Gastroenteritis in Diyala Governorate

Ali¹,

Hussein²,

kadim³

et al. 2020

ATMPH

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Human Bocavirus a newly detected parvovirus initially described as a respiratory pathogen, to be a possible causative agent of gastroenteritis in children. This cross-sectional study was carried out for patients with acute gastroenteritis who present in the Emergency Department of Pediatrics in Al-Batool Teaching Hospital for Maternity and Children in Baqubah city, during the period from July 2019 to March 2020. The study population consists of 100 children with diarrhea collected from children less than five years old (58 males, 42 females) to detect human Bocavirus by conventional polymerase chain reaction. Human Bocavirus was detected in 9 samples (9%), high infection observed in males (66.66%) and in age group 6-12 months (55.55%). Most infection occur in children whose mothers were illiterates and primary education with equal percentage (44.44%). The genetic analysis of the sequence of four samples (4 out of 9 positive case) showed three of them belong to HBoV-3,whereas the fourth belong to HBoV-2.

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“…Bocaparvoviruses in the genus Bocaparvovirus frequently infect young animals and humans through the fecal–oral tract, causing respiratory (Priestnall et al 2014 ; Verbeke et al 2019 ) and gastrointestinal symptoms (Bodewes et al 2014 ; Lin et al 2020 ), and have a broad host spectrum that infect animals such as cows, pigs, rats, cats, and dogs (Qiu et al 2007 ; Bodewes et al 2014 ; Lau et al 2017 ; Pfankuche et al 2016 ; Piewbang et al 2019 ). According to the latest report of the International Committee on Taxonomy of Viruses (ICTV), the genus Bocaparvovirus is divided into 25 species, most of which are novel bocavirus: Carnivore bocaparvovirus 1–6, Chiropteran bocaparvovirus 1–4, Lagomorph bocaparvovirus 1, Pinniped bocaparvovirus 1–2, Primate bocaparvovirus 1–2, Rodent bocaparvovirus 1–2, and Ungulate bocaparvovirus 1–8.…”

Section: Introductionmentioning

confidence: 99%

First detection of feline bocaparvovirus 2 and feline chaphamaparvovirus in healthy cats in Turkey

Hasan

Can-Şahna

2021

Vet Res Commun

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The pet cat’s population and the number of viruses that infect them are increasing worldwide. Recently, feline chaphamaparvovirus (FeChPV, also called fechavirus) and feline bocaparvovirus (FBoV) infections, which are novel parvovirus species, have been reported in cats from different geographic regions. Here, we investigated FBoV 1–3 and FeChPVs in healthy cats in Turkey using PCR, where nuclear phosphoprotein 1 (NP1) is targeted for FBoV and NP for FeChPV. For this purpose, oropharygeal swabs were obtained from 70 healthy cats with different housing status from June 15 to December 1, 2020. After PCR screening tests, six out of 70 cats (5/47 shelter cats; 1/23 domestic cats) were found to be positive for FBOV, while two were positive for FeChPV (1/47 shelter cats; 1/23 domestic cats). No cat was found in which both viruses were detected. The nucleotide (nt) sequence comparison in the 310 base pair (bp) NP gene of the two FeChPVs identified in this study shared a high identity with each other (95.0% nt and 99% aa identities) and with previously reported FeChPVs (92.4–97.1% nt and 98.1–99.0% aa identities), including 313R/2019/ITA, 49E/2019/ITA, VRI_849, 284R/2019/ITA, and IDEXX-1. Here, the near-full length (1489 nt, 495 amino acids-aa) of the VP2 gene of the FechaV/Tur-2020/68 isolate obtained from the study was also sequenced. The nt and aa identity ratio of this isolate with other FeChPVs was 98.0–98.5%-96–96.5%, respectively. Sequences of the 465 bp NP1 gene of the six Turkish FBoV strains shared high identities with each other (99.6–100% nt and 99.3–100% aa identities) and with those of FBoV-2 strains (97.8–99.1% nt and 98.0–100% aa identities), including 16SY0701, 17CC0505-BoV2, HFXA-6, and POR1. All FBoVs detected in this study were classified as genotype 2, similar to the study conducted in Japan and Portugal. Here, the NS1 (partial), NP1, VP1 and VP2 gene of the FBoV-2/TUR/2020–14 strain obtained from the study were also sequenced and the nt and aa sequences showed high identities to the above-mentioned FBoV-2 strain/isolates (> 96%, except for the aa ratio of strain 16SY0701). In conclusion, this study shows that FBoV and FeChPV are present in healthy cats in Turkey, and these viruses can be detected from oropharyngeal swabs. Our findings contribute to further investigation of the prevalence, genotype distribution, and genetic diversity of Turkish FBoVs and FeChPVs, adding to the molecular epidemiology of FBoV and FeChPVs worldwide.

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Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan

Cited by 12 publications

References 28 publications

Etiology of diarrheal disease among children under 5 years in Egypt: a high incidence of human bocavirus

Etiology of diarrheal disease among children under 5 years in Egypt: a high incidence of human bocavirus

Detecting and Genotyping of Human Bocavirus among Children with Gastroenteritis in Diyala Governorate

First detection of feline bocaparvovirus 2 and feline chaphamaparvovirus in healthy cats in Turkey

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