Genetic Diversity and Phylogenetic Analyses of Juglans regia L. (Juglandaceae) Populations Using RAPD, ISSR Markers and nrDNA ITS Regions

Sevindik, Emre; Okan, Kemalcan; Sevindik, Mustafa; Erċışlı, Sezai

doi:10.1007/s10341-023-00834-7

Cited by 2 publications

(1 citation statement)

References 56 publications

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“…Additionally, the microscopic characteristics of the seed coat are relatively stable and have significant value for the genetic classification of plants [8]. With the development of molecular biology, nrDNA-ITS has also been commonly used for species identification and phylogenetic Foods 2024, 13, 503 2 of 16 analysis in plants [9]. Therefore, in this study, the seed morphological and microscopic characteristics combined with nrDNA-ITS sequences were studied to reveal the species classification of edible Trichosanthes germplasm.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Edible Trichosanthes Germplasm on Its Seed Oil to Enhance Antioxidant and Anti-Aging Activity in Caenorhabditis elegans

Wang,

Li,

Zhu

et al. 2024

Foods

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The seeds of various Trichosanthes L. plants have been frequently used as snacks instead of for traditional medicinal purposes in China. However, there is still a need to identify the species based on seeds from Trichosanthes germplasm for the potential biological activities of their seed oil. In this study, 18 edible Trichosanthes germplasm from three species were identified and distinguished at a species level using a combination of seed morphological and microscopic characteristics and nrDNA-ITS sequences. Seed oil from the edible Trichosanthes germplasm significantly enhanced oxidative stress tolerance, extended lifespan, delayed aging, and improved healthspan in Caenorhabditis elegans. The antioxidant activity of the seed oil exhibits a significant positive correlation with its total unsaturated fatty acid content among the 18 edible Trichosanthes germplasm, suggesting a genetic basis for this trait. The biological activities of seed oil varied among species, with T. kirilowii Maxim. and T. rosthornii Harms showing stronger effects than T. laceribractea Hayata.

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Section: Introductionmentioning

confidence: 99%

Effect of Different Edible Trichosanthes Germplasm on Its Seed Oil to Enhance Antioxidant and Anti-Aging Activity in Caenorhabditis elegans

Wang,

Li,

Zhu

et al. 2024

Foods

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Development of SSR Markers for and Fingerprinting of Walnut Genetic Resources

Zhang,

Li,

et al. 2024

Forests

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Walnut is one of four major nuts in the world. China has abundant walnut germplasm resources, but there are still shortcomings in the identification of germplasm resources. This study used different walnut varieties as materials and developed 14 high-quality SSR molecular markers from 60 pairs of primers based on genome re-sequencing results. This study analyzed the genetic diversity of Chinese walnut genetic resources using 14 selected SSR markers. A total of 64 alleles were detected in 47 walnut resources, with an average of 4.571 alleles per locus. The variation range of polymorphism information content was 0.096~0.711, with an average value of 0.422. Cluster analysis, population genetic structure, and principal coordinate analysis divided 47 walnut resources into ordinary walnuts, Juglans hopeiensis, and Liaoyi 1. In addition, core SSR markers (Jr45, Jr40, Jr29, Jr35, and Jr11) were selected from 14 SSR markers, which were sufficient to distinguish 47 walnut resources. At the same time, 47 unique molecular fingerprints of walnuts were constructed using these core SSR markers. This study provides strong scientific support for rapid and efficient identification, germplasm innovation, and a variety of property protection of walnut germplasm.

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Genetic Diversity and Phylogenetic Analyses of Juglans regia L. (Juglandaceae) Populations Using RAPD, ISSR Markers and nrDNA ITS Regions

Cited by 2 publications

References 56 publications

Effect of Different Edible Trichosanthes Germplasm on Its Seed Oil to Enhance Antioxidant and Anti-Aging Activity in Caenorhabditis elegans

Effect of Different Edible Trichosanthes Germplasm on Its Seed Oil to Enhance Antioxidant and Anti-Aging Activity in Caenorhabditis elegans

Development of SSR Markers for and Fingerprinting of Walnut Genetic Resources

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