Genetic diversity and genotype multiplicity of Plasmodium falciparum infections in symptomatic individuals living in Bangui (CAR)

Dolmazon, Virginie; Matsika-Claquin, Marcelle Diane; Manirakiza, Alexandre; Yapou, Ferdinand; Nambot, Marina; Ménard, Didier

doi:10.1016/j.actatropica.2008.04.012

Cited by 19 publications

(23 citation statements)

References 23 publications

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“…This corroborates reports from other areas of Central Africa with intense malaria transmission [22,28]. In this study, the number of parasite genotypes carried by subjects with symptomatic infections was not influenced by age as shown elsewhere [29]. Irrespective of age, the sensitivity of the technique may not allow for the detection of minor genotypes when the parasite density of disease-inducing strain is high.…”

Section: Discussionsupporting

confidence: 90%

“…falciparum infection in this population is associated with the presence and growth of recently inoculated parasite strain(s) to which individuals have either not previously been exposed to or have not fully developed immunity [13,30]. This observation in Congolese population is in agreement with the study conducted in Bangui [29] and in Mozambique [31]. The msp-1 and msp-2 genes appeared polymorphic, and both taken together, the relation between parasite density and multiplicity of infection become more pronounced.…”

Section: Discussionsupporting

confidence: 84%

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Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo

Mayengue

Ndounga²,

Malonga

et al. 2011

Malar J

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BackgroundThe characterization of malaria parasite populations circulating in an area is part of site characterization, as a basis for evaluating the impact of malaria interventions on genetic diversity, parasite species, and multiplicity of infection. The present study was aimed at analysing genetic diversity of Plasmodium falciparum merozoite surface proteins 1 and 2 (MSP-1 and MSP-2) and to determine the multiplicity of infection in clinical isolates collected from children living in the Southern district of Brazzaville in the Republic of Congo.MethodsA total of 125 isolates from patients with uncomplicated malaria attending Terinkyo and Madibou health centres were collected between January and June 2005 while evaluating the therapeutic efficacy of amodiaquine-artesunate combination. DNA was extracted and msp-1 and msp-2 genes were genotyped using allele-specific nested-PCR.ResultsOut of 468 distinct fragments detected, 15 msp-1 and 20 msp-2 genotypes were identified. For the msp-1 gene, K1 family was the predominant allelic type carried alone or in association with RO33 and Mad20 types, whereas the 3D7 family was the most prevalent in the msp-2 gene. Overall, the mean multiplicity of infection was 2.2. Out of 125 samples, 104 (83%) harboured more than one parasite genotype. There was no statistical significant difference in the multiplicity of infection by either sex or age of patients. However, a statistically significant correlation was found between parasite densities and the number of genotypes.ConclusionPolymorphism in P. falciparum clinical isolates from Brazzaville was high and mainly of multiple clones. The basis for the positive association between parasite densities and multiplicity of infection is discussed.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 84%

Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo

Mayengue

Ndounga²,

Malonga

et al. 2011

Malar J

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“…The results of this study show thatage has no associationon multiplicity of infection, similar to reports from others studies [33]. Onthecontrary, this finding contrasts with reports from Brazzaville, Republic of Congo [34].…”

Section: Discussionsupporting

confidence: 77%

Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

et al. 2017

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BackgroundIn malaria endemic regions, Plasmodium falciparum infection is characterized by extensive genetic diversity. Describing this diversity provides important information about the local malaria situation. This study was conducted to evaluate the extent of genetic diversity of P. falciparum in Pawe district, North West Ethiopia, using the highly polymorphic merozoite surface protein 2 gene.MethodsAtotal of 92 isolates from patients with uncomplicated P. falciparum attending Pawe Health Centre were collected from September to December 2013. Genomic DNA was extracted using the Chelex method and analyzed by length polymorphism following gel electrophoresis of DNA products from nested PCR of msp2 (block 3), targeting allelic families of FC27 and 3D7/IC.ResultsThere were twenty-two different msp2 alleles, 11 corresponding to the 3D7/ IC and 11 to the FC27 allelic family. The frequency of isolates of the msp2 3D7/IC allelic familywas higher (51%) compared to FC27 (49%). The majority of the isolates (76%) contained multiple infections andthe overall mean multiplicity of infection was 2.8 (CI 95% 2.55–3.03). The heterozygosity index was 0.66 for msp2. There was no statically significant difference in the multiplicity of infection by age or parasite density.ConclusionsThe results of this study show that P.falciparum polymorphismsare extensive in Northwest Ethiopia and most of the infections are composed of multiple clones.

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“…The Central African Republic (CAR), located in tropical Africa, is considered to be an area of high endemicity for the main infectious diseases, including infections with HIV [15], hepatitis B virus and other hepatotropic viruses [16,17], yellow fever [18], malaria [19], tuberculosis [20] and other infections [21-23]. No HEV epidemics were documented in the CAR before 2001, although HEV antibodies were detected in 24% of young sexually active adults [24].…”

Section: Introductionmentioning

confidence: 99%

Clinical and epidemiological aspects of a hepatitis E outbreak in Bangui, Central African Republic

2011

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BackgroundOutbreaks of hepatitis E frequently occur in tropical developing countries during the rainy season due to overflowing drains, short-circuiting of networks of clean water and use of contaminated water from wells. Hepatitis E virus (HEV) infections are usually accompanied by general symptoms of acute liver disease. This study was conducted to define the clinical and epidemiological aspects of the HEV outbreak that occurred in May 2004 in Bangui.MethodsBlood samples were collected from 411 patients aged 1-87 years, most of whom presented with jaundice, asthenia or signs of uncomplicated malaria, for a transversal study from June 2004 to September 2005. Patients were recruited at 11 health care centres, including two referral hospitals, after they had given informed consent. The diagnosis of HEV was made with a commercial ELISA test to detect IgM and/or IgG antibodies. HEV RNA was amplified by RT-PCR to confirm the presence of the viral genome.ResultsThe most frequent clinical signs found were jaundice (93.4%), vomiting (50.7%), hepatalgia (47.4%), hepatomegaly (30.9%) and asthenia (26.8%), which are the general clinical signs of hepatic disease. Acute hepatitis E was found in 213 patients (51.8%) who were positive for HEV IgM antibodies. The IgG anti-HEV seroprevalence during this outbreak was high (79.5%). The age group 18-34 years was more frequently infected (91.2%) than those aged 1-17 (78.0%) or over 34 (64.9%) (p < 10-6). RT-PCR performed on 127 sera from the 213 IgM-HEV-positive patients was amplified, and the presence of the viral genome was found in 65 samples.ConclusionAlthough no specific clinical signs exist for hepatitis E infection, people presenting with jaundice, vomiting, hepatalgia, asthenia, hepatomegaly or distended abdomen with no signs of uncomplicated malaria in tropical developing countries should be sent to a laboratory for testing for hepatitis E.

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Genetic diversity and genotype multiplicity of Plasmodium falciparum infections in symptomatic individuals living in Bangui (CAR)

Cited by 19 publications

References 23 publications

Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo

Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo

Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

Clinical and epidemiological aspects of a hepatitis E outbreak in Bangui, Central African Republic

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