Genetic diversity and evolution of two capsid protein genes of citrus tristeza virus isolates from China

Wu, Guanwei; Tang, Min; Wang, Guoping; Jin, Fengyin; Yang, Zuokun; Liu, Cheng; Hóng, Ní

doi:10.1007/s00705-014-2281-2

Cited by 11 publications

(5 citation statements)

References 28 publications

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“…The CTV complexes investigated showed distinct genetic profiles, even when they are from the same region of origin, as for the trees 89A and 89BL. These results are similar to findings published elsewhere where the structure of a CTV population was not associated with geographic origin, but with gene flow, negative selection, and frequent recombination events (Wu et al 2015). In contrast, the similarity between mildly virulent CTV isolates from several regions of the world indicated that some of these isolates may remain relatively conserved throughout the years, independently of geographic origin (Albiach-Martí et al 2000b).…”

Section: Recombinant Genotypessupporting

confidence: 90%

Severity and genetic diversity of Citrus tristeza virus complexes infecting ‘Pêra Bianchi’ sweet orange in Paraná, Brazil

Giampani

Santos

Silva

et al. 2017

Trop. plant pathol.

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The aggressiveness and genetic diversity of the Citrus tristeza virus (CTV) infecting sweet orange 'Pêra Bianchi' (Citrus sinensis) trees were evaluated under the environmental conditions of the state of Paraná, Brazil. Disease severity was assessed based on stem pitting symptoms in the citrus trees, while genetic diversity was determined by sequencing the virus coat protein (CP) gene. Stem pitting symptoms were less severe in trees maintained under greenhouse conditions and field experimental plots in the municipalities of Paranavaí and Umuarama, where the temperature is higher. In contrast, the most severe stem pitting symptoms were observed in trees from experimental orchards established in colder areas (i.e. municipalities of Arapongas, Cascavel and Londrina), suggesting a temperature effect on symptom development. Analysis of CP sequences indicated intra-and interspecific diversity of the CTV complex for most of the trees included in this study. Recombination analyses of the CP gene also allowed to identify recombinants with parentals related to PIAC-5, a mild isolate, and 1BL-3, found in trees from a field plot in Londrina. The CTV complex infecting 'Pêra Bianchi' trees under field conditions showed a high degree of genetic diversity, implying the possibility of multiple infections or even a dynamic rearrangement of the genotypes into different CTV complexes under the environmental conditions of the state of Paraná.

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Section: Recombinant Genotypessupporting

confidence: 90%

Severity and genetic diversity of Citrus tristeza virus complexes infecting ‘Pêra Bianchi’ sweet orange in Paraná, Brazil

Giampani

Santos

Silva

et al. 2017

Trop. plant pathol.

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“…The p25 gene encoding the major capsid protein (CP) [12] has been used for molecular characterization and typing of CTV isolates around the world [13, 14]. Generally, a correlation between geographical origin and/or biological characteristics of CTV isolates and their CP gene sequences was observed [15–22]. Thus, the CP gene of a large number of CTV isolates with various biological properties from many different citrus growing regions of the world has been sequenced and submitted to GenBank.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey

Erkiş-Güngör

Çevik

2019

Advances in Virology

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The presence of Citrus tristeza virus (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates have not been studied to date in Turkey. In this study, molecular characteristics of 15 CTV isolates collected from different citrus growing regions of Turkey were determined by amplification, cloning, and sequencing of their major coat protein (CP) genes. The sequence analysis showed that the CP genes were highly conserved among Turkish isolates. However, isolates from different regions showed more genetic variation than isolates from the same region. Turkish isolates were clustered into three phylogenetic groups showing no association with geographical origins, host, or symptoms induced in indicator plants. Phylogenetic analysis of Turkish isolates with isolates from different citrus growing regions of the world including well-characterized type isolates of previously established strain specific groups revealed that some Turkish isolates were closely related to severe quick decline or stem pitting isolates. The results demonstrated that although CTV isolates from Turkey are considered biologically mild, majority of them contain severe components potentially causing quick decline or stem pitting.

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“…The exact parents for these recombinants need to be clarified as more genomic sequences available from a wide host source. The data will help to understand whether an ancient recombination event occurred before the recombinants spread worldwide, as previously suggested for CTV (Roy et al, 2005;Rubio et al, 2013;Wu G. et al, 2015). Most of the recombination events occurred in the region following the viral ORF1b.…”

Section: Discussionmentioning

confidence: 59%

Next-Generation Sequencing Combined With Conventional Sanger Sequencing Reveals High Molecular Diversity in Actinidia Virus 1 Populations From Kiwifruit Grown in China

et al. 2020

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Kiwifruit (Actinidia spp.) is native to China. Viral disease–like symptoms are common on kiwifruit plants. In this study, six libraries prepared from total RNA of leaf samples from 69 kiwifruit plants were subjected to next-generation sequencing (NGS). Actinidia virus 1 (AcV-1), a tentative species in the family Closteroviridae, was discovered in the six libraries. Two full-length and two near-full genome sequences of AcV-1 variants were determined by Sanger sequencing. The genome structure of these Chinese AcV-1 variants was identical to that of isolate K75 and consisted of 12 open reading frames (ORFs). Analyses of these sequences together with the NGS-derived contig sequences revealed high molecular diversity in AcV-1 populations, with the highest sequence variation occurring at ORF1a, ORF2, and ORF3, and the available variants clustered into three phylogenetic clades. For the first time, our study revealed different domain compositions in the viral ORF1a and molecular recombination events among AcV-1 variants. Specific reverse transcriptase–polymerase chain reaction assays disclosed the presence of AcV-1 in plants of four kiwifruit species and unknown Actinidia spp. in seven provinces and one city.

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Genetic diversity and evolution of two capsid protein genes of citrus tristeza virus isolates from China

Cited by 11 publications

References 28 publications

Severity and genetic diversity of Citrus tristeza virus complexes infecting ‘Pêra Bianchi’ sweet orange in Paraná, Brazil

Severity and genetic diversity of Citrus tristeza virus complexes infecting ‘Pêra Bianchi’ sweet orange in Paraná, Brazil

Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey

Next-Generation Sequencing Combined With Conventional Sanger Sequencing Reveals High Molecular Diversity in Actinidia Virus 1 Populations From Kiwifruit Grown in China

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