Genetic diversity and evolution of Mycoplasma capricolum subsp. capripneumoniae strains from eastern Africa assessed by 16S rDNA sequence analysis

Heldtander, M.; Wesonga, Hezron; Bölske, Göran; Pettersson, Bertil; Johansson, Karl‐Erik

doi:10.1016/s0378-1135(00)00290-x

Cited by 32 publications

(20 citation statements)

References 30 publications

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“…Most of them had already been analysed in previous studies [7,8,16] and corresponding 16S rDNA and H2 types are presented whenever available (Table 1). Mycoplasma strains were cultured in modified Hayflick's broth [6] at 37°C, 5% CO 2 and were harvested in the late exponential phase of growth.…”

Section: Methodsmentioning

confidence: 99%

Multi-locus sequence analysis of mycoplasma capricolum subsp. capripneumoniae for the molecular epidemiology of contagious caprine pleuropneumonia

Manso-Silván

Dupuy

Chu

et al. 2011

Vet Res

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Mycoplasma capricolum subsp. capripneumoniae (Mccp) is the causative agent of contagious caprine pleuropneumonia (CCPP), a devastating disease of domestic goats. The exact distribution of CCPP is not known but it is present in Africa and the Middle East and represents a significant threat to many disease-free areas including Europe. Furthermore, CCPP has been recently identified in Tajikistan and China. A typing method with an improved resolution based on Multi-Locus Sequence Analysis (MLSA) has been developed to trace new epidemics and to elucidate whether the recently identified cases in continental Asia were due to recent importation of Mccp. The H2 locus, a polymorphic region already in use as a molecular marker for Mccp evolution, was complemented with seven new loci selected according to the analysis of polymorphisms observed among the genome sequences of three Mccp strains. A total of 25 strains, including the two new strains from Asia, were analysed by MLSA resulting in the discrimination of 15 sequence types based on 53 polymorphic positions. A distance tree inferred from the concatenated sequences of the eight selected loci revealed two evolutionary lineages comprising five groups, which showed good correlation with geographic origins. The presence of a distinct Asian cluster strongly indicates that CCPP was not recently imported to continental Asia. It is more likely that the disease has been endemic in the area for a long time, as supported by historical clinical descriptions. In conclusion, this MLSA strategy constitutes a highly discriminative tool for the molecular epidemiology of CCPP.

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Section: Methodsmentioning

confidence: 99%

Multi-locus sequence analysis of mycoplasma capricolum subsp. capripneumoniae for the molecular epidemiology of contagious caprine pleuropneumonia

Manso-Silván

Dupuy

Chu

et al. 2011

Vet Res

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“…The Mccp organism is genetically and antigenically very closely related to Mycoplasma capricolum subspecies capricolum (Mcc), the causative agent of respiratory pneumonia in goats and sheep (Bascunana et al 1994;Monnerat et al 1999). Mccp is a member of the so-called Mycoplasma mycoides cluster (Heldtander et al 2001), and is difficult to identify through ordinary procedures, as it belongs to a group of five closely related ruminant pathogenic Mycoplasmas Thiaucourt et al 2011), that share similar serological cross reactions and similar biochemical features sometimes leading to the erroneous diagnosis of CCPP (Cottew et al 1987;Erno 1987;Adehan et al 2006). F38 was taxonomically reported as a subspecies of Mycoplasma capricolum and was recently renamed as Mycoplasma capricolum subsp.…”

Section: Causative Agent and Its Relationship To Other Mycoplasmasmentioning

confidence: 99%

“…Mycoplasmas are phylogenetically related to gram positive bacteria with low G + C content (Razin et al 1983;Bascunana et al 1994). The Mycoplasma mycoides cluster has two rRNA operons in which intraspecific variations have been demonstrated (Heldtander et al 2001). Mccp was once thought to be a homogenous taxon (Abu-Groun et al 1994;Nicholas 2002a;Manso-Silvan et al 2007), but the discovery of two molecular markers showed some degree of heterogeneity among strains that opened a further channel for studies on the molecular epidemiology of CCPP .…”

Section: Genetic Diversity Of Mycoplasma Capricolum Subsp Capripneummentioning

confidence: 99%

“…Mccp was once thought to be a homogenous taxon (Abu-Groun et al 1994;Nicholas 2002a;Manso-Silvan et al 2007), but the discovery of two molecular markers showed some degree of heterogeneity among strains that opened a further channel for studies on the molecular epidemiology of CCPP . Polymorphisms in Mccp strains can be used as epidemiological markers for CCPP in smaller geographical areas and to study the molecular evolution of this species (Heldtander et al 2001). Eleven polymorphic positions were observed in the sequence of 2400 bp long fragments, obtained from 19 Mccp strains from various geographical locations (Lorenzon et al 2002).…”

Section: Genetic Diversity Of Mycoplasma Capricolum Subsp Capripneummentioning

confidence: 99%

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Contagious caprine pleuropneumonia and its current picture in Pakistan: a review

Samiullah¹

2013

Vet. Med. - Czech

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Contagious caprine pleuropneumonia (CCPP) is caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp) which belongs to the Mycoplasma mycoides cluster, a group of five closely related Mycoplasmas, pathogenic to ruminants. The true lesions of CCPP are restricted to the alveolar tissues of infected goats, which distinguish it from other respiratory diseases of small ruminants caused by members of the Mycoplasma mycoides cluster. The typical signs of CCPP are an accumulation of pleural fluid, unilateral hepatisation, adhesions, pleurisy and pleuropneumonia which clearly differentiate it from "MAKePS" syndrome caused by Mycoplasma mycoides subsp. capri (Mmc). The available literature on CCPP shows that so far in Pakistan, the true causative agent (Mccp) of this disease has only been isolated in the Pashin District of Balochistan and that the disease is more frequently confused with other respiratory diseases of goat caused by the Mycoplasma mycoides cluster. The lack of suitable techniques and extensive knowledge in the field is a big limitation for the isolation and characterisation of Mccp from prevailing CCPP-like cases in the goat population of Pakistan.

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“…Heldtander et al (2002) showed that RAPD allows avian mycoplasma isolates to be distinguished with much more sensitivity than RFLP analysis. The simple and rapid DNA preparation associated with the technique should allow cost effective analyses of numerous f ield isolates, providing signif icant new Six Mycoplasma pullorum isolates analysed with three arbitrary primers (M16SPCR5', M13F and S1OLIGO3').…”

Section: )mentioning

confidence: 99%

Short communication. Strain heterogeneity in Mycoplasma pullorum isolates identified by random amplified polymorphic DNA techniques

Lobo

Garcı́a

Moscoso

et al. 2004

Span. j. agric. res.

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Mycoplasmas are of great concern in veterinary medicine since they are responsible for economic losses in animal production. The most important avian mycoplasmas that cause severe respiratory disease in chickens and turkeys are Mycoplasma gallisepticum and M. synoviae. M. iowae and M. meleagrides affect turkeys alone (Fan et al., 1995).Chronic respiratory syndrome (CRS) in birds is a disease with a multifactorial aetiology. M. gallisepticum and M. synoviae are the most important pathogens in CRS, although Citti and Rosengarten (1997) indicate that other species may also participate in the disease.In Cuba, CRS causes losses of some US$1 million per year. Recent data (Instituto Medicina Veterinaria, 2003) show that in the chicken-raising industry CRS is the second most important cause of death induced by an infectious agent, with an incidence of 12.67%.In 1997, during field investigations of an outbreak of CRS in western Cuba, mycoplasmas were isolated from chickens with respiratory problems. Lobo (1998) showed the incidence of M. gallisepticum, M. synoviae and M. pullorum in these diseased birds to be 40%, 30% and 22% respectively.For a long time M. pullorum was thought to be a saprophytic organism of the avian respiratory tract (Jordan, 1985), but since 1995 it has been isolated alone and with other mycoplasma species from birds with CRS (Bencina et al., 1987). Two isolates with the biochemical and serological characteristics of M. pullorum have been isolated from adult turkeys and dead turkey embryos in France (Moalic et al., 1997). AbstractMycoplasmas were isolated from chickens with respiratory problems during field investigations of a concentrated respiratory disease outbreak in western Cuba, 1997. A high percentage of mycoplasma cultures from tracheas and air-sac lesions yielded pure cultures of Mycoplasma pullorum. The aim of the present work was to investigate the heterogeneity among M. pullorum isolates from Cuba and strains from other countries using random amplified polymorphic DNA (RAPD) techniques. The results show that the RAPD method may be a useful identification tool for studying the epidemiology of poultry mycoplasmosis in Cuba.Key words: avian mycoplasmosis, RAPD, chronic respiratory syndrome. ResumenNota corta. Análisis de heterogeneidad entre aislados de Mycoplasma pullorum por medio de técnicas de amplificación de DNA polimórficoEn 1997 se aislaron micoplasmas en el curso de investigaciones de campo en pollos con un brote de enfermedad respiratoria localizada en la zona oeste de Cuba. Un alto porcentaje de los cultivos de micoplasmas procedentes de lesiones de tráquea y alveolos revelaron la presencia de Mycoplasma pullorum. El objetivo de este trabajo fue investigar la heterogeneidad de los aislados cubanos entre sí y con cepas de otros países, mediante la técnica RAPD (DNA polimórfico amplificado al azar). Los resultados muestran que la técnica RAPD puede ser una herramienta de identificación útil en el estudio de la epidemiología de la micoplasmosis aviar en Cuba.Palabras clave: micop...

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Genetic diversity and evolution of Mycoplasma capricolum subsp. capripneumoniae strains from eastern Africa assessed by 16S rDNA sequence analysis

Cited by 32 publications

References 30 publications

Multi-locus sequence analysis of mycoplasma capricolum subsp. capripneumoniae for the molecular epidemiology of contagious caprine pleuropneumonia

Multi-locus sequence analysis of mycoplasma capricolum subsp. capripneumoniae for the molecular epidemiology of contagious caprine pleuropneumonia

Contagious caprine pleuropneumonia and its current picture in Pakistan: a review

Short communication. Strain heterogeneity in Mycoplasma pullorum isolates identified by random amplified polymorphic DNA techniques

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