Genetic diversity and differentiation among populations of the Indian eri silkworm, Samia cynthia ricini, revealed by ISSR markers

Vijayan, K.; Anuradha, H. J.; Nair, C. V.; Pradeep, A. R.; Awasthi, A. K.; Saratchandra, B.; Rahman, S. A. S.; Singh, Kamendra; Chakraborti, Rajat K.; Urs, S. Raje

doi:10.1673/2006_06_30.1

Cited by 16 publications

(15 citation statements)

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“…Twenty primers showed reproducible amplification and were used for the whole study. PCR conditions were kept the same as explained earlier (Vijayan et al 2006) and the products were viewed in UV light after 1.5% agarose gel electrophoresis. The profile was scored into a binary matrix where '1' represents presence of the marker and '0' for absence of the marker and was used for deriving a genetic distance matrix and dendrogram (Nei 1978).…”

Section: Methodsmentioning

confidence: 99%

“…Presumably, altitude induces genetic as well as phenotypic alterations in the populations, which was analyzed here by inter simple sequence repeat (ISSR) marker system. Simple sequence repeats (SSRs) and ISSRs were used for genetic analysis in silkworms and other animals (Goldsmith et al 2005;Behura 2006;Vijayan et al 2006). The ISSR primers contain microsatellite motifs and three anchoring nucleotides at the 5′ terminal end and were used in genetic analysis (Zietkiewicz et al 1994;Santana et al 2009) and marker-association studies (Pradeep et al 2005a;Srivastava et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Genetic evaluation of eri silkworm Samia cynthia ricini: ISSR loci specific to high and low altitude regimes and quantitative attributes

et al. 2011

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Genetic structure of populations is under constant pressure from varying geographical conditions that induce phenotypic plasticity in insects. Spatial distribution of 15 populations of Indian eri silkworm, Samia cynthia ricini originated at various altitudes of sub Himalayas based on Euclidean distance realized from yield attributes showed two population clusters irrespective of their place of origin and altitude. However, DNA amplification profile by inter SSR (ISSR) markers showed genetic variations among the populations depend on low and high altitudes. One ISSR locus each specific to high and low altitude population was identified. The locus from high altitude showed deviation from Hardy-Weinberg equilibrium but that from low altitude was in neutrality suggests that the high altitude loci could be under pressure from the altitudinal variations. In association with different yield traits, 18 loci were identified. Of which, three markers showed association with more than one trait indicative of pleiotropic influence. Stepwise addition of markers enhanced the correlation between markers and the associated trait pointed to polygenic influence. Association of markers with altitude and yield traits suggests an imperative relation of rare genetic loci with gene-environment interaction and phenotypic variability in S. c. ricini.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic evaluation of eri silkworm Samia cynthia ricini: ISSR loci specific to high and low altitude regimes and quantitative attributes

et al. 2011

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“…Philosamia cynthia ricini, the Indian eri silkworm, contributes significantly to the production of commercial silk in India and China (Vijayan et al 2006). The disease caused by P. cynthia ricini NPV (PhcyNPV) is a major threat to the silk-spinning industry.…”

Section: Analysis Of Oral Infection and Helicase Gene Of The Nucleopomentioning

confidence: 99%

Analysis of oral infection andhelicasegene of the nucleopolyhedroviruses isolated fromPhilosamia cynthia riciniandAntheraea pernyi

Chen

Shen

et al. 2008

Biocontrol Science and Technology

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“…As ISSR, the dominant marker system is simpler to use though not entailing prior information on target regions (Zietkiewicz et al , 1994; Nagaraju et al , 2002), it has been extensively employed for identifying inter-and intra-populational genetic variability in several insect species, including domestic and wild silkworms (Ehtesham et al , 1995; Pradeep et al , 2005, 2007, 2008; Vijayan et al , 2005, 2006; Santana et al , 2009; Saravanakumar et al , 2010). Hence the ISSR marker system has been selected for genetic analysis of the eri populations.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of scattered populations of the Indian eri silkworm, Samia cynthia ricini Donovan: differentiation of subpopulations

Pradeep¹,

Jingade²,

Singh

et al. 2011

Genet. Mol. Biol.

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Deforestation and exploitation has led to the fragmentation of habitats and scattering of populations of the economically important eri silkworm, Samia cynthia ricini, in north-east India. Genetic analysis of 15 eri populations, using ISSR markers, showed 98% inter-population, and 23% to 58% intra-population polymorphism. Nei’s genetic distance between populations increased significantly with altitude (R2 = 0.71) and geographic distance (R2 = 0.78). On the dendrogram, the lower and upper Assam populations were clustered separately, with intermediate grouping of those from Barpathar and Chuchuyimlang, consistent with geographical distribution. The Nei’s gene diversity index was 0.350 in total populations and 0.121 in subpopulations. The genetic differentiation estimate (Gst) was 0.276 among scattered populations. Neutrality tests showed deviation of 118 loci from Hardy-Weinberg equilibrium. The number of loci that deviated from neutrality increased with altitude (R2 = 0.63). Test of linkage disequilibrium showed greater contribution of variance among eri subpopulations to total variance. D’2IS exceeded D’2ST, showed significant contribution of random genetic drift to the increase in variance of disequilibrium in subpopulations. In the Lakhimpur population, the peripheral part was separated from the core by a genetic distance of 0.260. Patchy habitats promoted low genetic variability, high linkage disequilibrium and colonization by new subpopulations. Increased gene flow and habitat-area expansion are required to maintain higher genetic variability and conservation of the original S. c. ricini gene pool.

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Genetic diversity and differentiation among populations of the Indian eri silkworm, Samia cynthia ricini, revealed by ISSR markers

Cited by 16 publications

References 1 publication

Genetic evaluation of eri silkworm Samia cynthia ricini: ISSR loci specific to high and low altitude regimes and quantitative attributes

Genetic evaluation of eri silkworm Samia cynthia ricini: ISSR loci specific to high and low altitude regimes and quantitative attributes

Analysis of oral infection andhelicasegene of the nucleopolyhedroviruses isolated fromPhilosamia cynthia riciniandAntheraea pernyi

Genetic analysis of scattered populations of the Indian eri silkworm, Samia cynthia ricini Donovan: differentiation of subpopulations

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