Analysis of oral infection andhelicasegene of the nucleopolyhedroviruses isolated fromPhilosamia cynthia riciniandAntheraea pernyi

Chen, Yan; Li, Bing; Shen, Weide; Zhu, Shanying; Wu, Yan; Wang, Wenbing

doi:10.1080/09583150802416593

Cited by 1 publication

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AnpeNPV caused 57% mortality in larvae of P. cynthia rici , whereas PcrNPV did not kill the larvae of A. pernyi [19]. Hence, the six genes ( pif 0 to 5) required for infectivity via the oral route were analyzed.…”

Section: Resultsmentioning

confidence: 99%

Analysis of the genomic sequence of Philosamia cynthia nucleopolyhedrin virus and comparison with Antheraea pernyinucleopolyhedrin virus

Qian

Zhang

et al. 2013

BMC Genomics

Self Cite

View full text Add to dashboard Cite

BackgroundTwo species of wild silkworms, the Chinese oak silkworm (Antheraea pernyi) and the castor silkworm Philosamia cynthia ricini, can acquire a serious disease caused by Nucleopolyhedrin Viruses (NPVs) (known as AnpeNPV and PhcyNPV, respectively). The two viruses have similar polyhedral morphologies and their viral fragments share high sequence similarity. However, the physical maps of the viral genomes and cross-infectivity of the viruses are different. The genome sequences of two AnpeNPV isolates have been published.ResultsWe sequenced and analyzed the full-length genome of PhcyNPV to compare the gene contents of the two viruses. The genome of PhcyNPV is 125, 376 bp, with a G + C content of 53.65%, and encodes 138 open reading frames (ORFs) of at least 50 amino acids (aa) (GenBank accession number: JX404026). Between PhcyNPV and AnpeMNPV-L and -Z isolates, 126 ORFs are identical, including 30 baculovirus core genes. Nine ORFs were only found in PhcyNPV. Four genes, cath, v-chi, lef 10 and lef 11, were not found in PhcyNPV. However, most of the six genes required for infectivity via the oral route were found in PhcyNPV and in the two AnpeNPV isolates, with high sequence similarities. The pif-3 gene of PhcyNPV contained 59 aa extra amino acids at the N-terminus compared with AnpeNPV.ConclusionsMost of the genes in PhcyNPV are similar to the two AnpeNPV isolates, including the direction of expression of the ORFs. Only a few genes were missing from PhcyNPV. These data suggest that PhcyNPV and AnpeNPV might be variants of each other, and that the differences in cross-infection might be caused by gene mutations.

show abstract

Section: Resultsmentioning

confidence: 99%