Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients

Silva, Rosana Bellan de Oliveira; Fusco‐Almeida, Ana Marisa; Matsumoto, Marcelo Teruyuki; Baeza, Lílian Cristiane; Benaducci, Tatiane; Mendes-Giannini, María José Soares

doi:10.1590/s1517-83822008000300033

Cited by 10 publications

(4 citation statements)

References 54 publications

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“…reported that those samples were susceptible to Amphotericin B, but susceptible to Fluconazole. Silva, et al ., (15) also evaluate the antifungal susceptibility profile of T. asahii clinical isolates. The isolates had reduced susceptibility in vitro to all drugs, showing 4-6 times higher MICs to itraconazole and 3-4 times to 5-flucytosine compared of MICs breakpoint values.…”

Section: Discussionmentioning

confidence: 99%

Antifungal susceptibility profile of Trichosporon isolates: correlation between CLSI and etest methodologies

Lemes¹,

Lyon²,

Moreira³

et al. 2010

Braz. J. Microbiol.

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The aim of the present study was to evaluate the antifungal susceptibility profile of Trichosporon species isolated from different sources employing the Clinical and Laboratory Standards Institute (CLSI) method and E-test method. Thirty-four isolates of Trichosporon spp. and six CBS reference samples were tested for their susceptibility to Amphotericin B, 5-flucytosine, Fluconazole, Itraconazole, Voriconazole and Terbinafine. All species showed high Minimun Inhibitory Concentrations (MIC) for Itraconazole and susceptibility to Fluconazole, The comparison among the results obtained by the CLSI method and E-test revealed larger discrepancies among 5-flucytosine and Itraconazole. The present work provides epidemiological data that could influence therapeutic choices. Furthermore, the comparison between different methodologies could help to analyze results obtained by different laboratories.

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Section: Discussionmentioning

confidence: 99%

Antifungal susceptibility profile of Trichosporon isolates: correlation between CLSI and etest methodologies

Lemes¹,

Lyon²,

Moreira³

et al. 2010

Braz. J. Microbiol.

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“…clinical isolates resistant to amphotericin B, azoles and caspofungin, and even a multidrug-resistant phenotype (Di Bonaventura et al, 2006;Silva et al, 2008;Wolf et al, 2001). Biofilms produced by Trichosporon spp.…”

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confidence: 99%

Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals

Cordeiro

Serpa

Alexandre

et al. 2015

Journal of Medical Microbiology

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The aim of this study was to determine experimental conditions for in vitro biofilm formation of clinical isolates of Trichosporon inkin, an important opportunistic pathogen in immunocompromised patients. Biofilms were formed in microtitre plates in three different media (RPMI, Sabouraud and CLED), with inocula of 10 4 , 10 5 or 10 6 cells ml 21, at pH 5.5 and 7.0, and at 35 and 28 8C, under static and shaking conditions for 72 h. Growth kinetics of biofilms were evaluated at 6, 24, 48 and 72 h. Biofilm milieu analysis were assessed by counting viable cells and quantification of nucleic acids released into biofilm supernatants. Biofilms were also analysed for proteolytic activity and antifungal resistance against amphotericin B, caspofungin, fluconazole, itraconazole and voriconazole. Finally, ultrastructural characterization of biofilms formed in microtitre plates and catheter disks was performed by scanning electron microscopy. Greater biofilm formation was observed with a starter inoculum of 10 6 cells ml 21, at pH 7.0 at 35 8C and 80 r.p.m., in both RPMI and Sabouraud media. Growth kinetics showed an increase in both viable cells and biomass with increasing incubation time, with maximum production at 48 h. Biofilms were able to disperse viable cells and nucleic acids into the supernatant throughout the developmental cycle. T. inkin biofilms produced more protease than planktonic cells and showed high tolerance to amphotericin B, caspofungin and azole derivatives. Mature biofilms were formed by different morphotypes, such as blastoconidia, arthroconidia and hyphae, in a strain-specific manner. The present article details the multicellular lifestyle of T. inkin and provides perspectives for further research.

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“…There is little data available regarding T. asahii's susceptibility to antifungal drugs in Brazil. The emergence of T. asahii with reduced susceptibility to common antifungal drugs is cause for concern 14 . In our study, resistance to fluconazole was detected, but the strain was susceptible to amphotericin B.…”

Section: Resultsmentioning

confidence: 99%

Isolation and antifungal susceptibility testing of Trichosporon asahii in Ceará, Brazil

Menezes

Marinho

Ângelo

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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SUMMARYTrichosporon spp. are yeasts capable of causing invasive disease, which mainly affect immunocompromised patients. A clinical strain of T. asahii was isolated from the blood cultures of patients admitted to the General Hospital of Fortaleza. Susceptibility tests were conducted by disk diffusion and broth microdilution. The isolated strain of T. asahii was resistant to fluconazole. The patient used amphotericin B and caspofungin in order to facilitate the microbiological cure. It was the first isolation and identification of T. asahii in blood culture in Ceará, Brazil.

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Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients

Cited by 10 publications

References 54 publications

Antifungal susceptibility profile of Trichosporon isolates: correlation between CLSI and etest methodologies

Antifungal susceptibility profile of Trichosporon isolates: correlation between CLSI and etest methodologies

Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals

Isolation and antifungal susceptibility testing of Trichosporon asahii in Ceará, Brazil

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