Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals

Cordeiro, Rossana de Aguiar; Serpa, Rosana; Alexandre, Camila Flávia Uchoa; Marques, Francisca Jakelyne de Farias; Melo, Charlline Vládia Silva de; Franco, Jônatas da Silva; Evangelista, Antonio José de Jesus; Camargo, Zoilo Pires de; Brilhante, Raimunda Sâmia Nogueira; Rocha, Marcos Fábio Gadelha; Moreira, José Luciano Bezerra; Bandeira, Tereza de Jesus Pinheiro Gomes; Sidrim, José Júlio Costa

doi:10.1099/jmm.0.000159

Cited by 34 publications

(21 citation statements)

References 50 publications

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“…Concentration of rhamnolipid and amphotericin B corresponding to the lowest FIC had an effect equal to that of the individual substances applied in their MIC. Cordeiro et al (2015) and Liao et al (2015) reported similar results; an almost complete suppression of biofilm formation needed a much higher concentration of amphotericin B than inhibition of planktonic cells.…”

Section: Effect Of Rhamnolipid and Amphotericin B On Biofilm Formationsupporting

confidence: 76%

“…Adhesion of microbial cells is linked with cell phenotype variations, which are usually manifested by new metabolic pathways, enhanced virulence and, as in the basis of this work, increased resistance to antimicrobial compounds (Nikolaev and Plakunov 2007). Biofilm resistance to various types of antimicrobial agents including biocides, antibiotics, and antiseptics is well reported (Cordeiro et al 2015;Donlan and Costerton 2002;Gilbert et al 2002;Leung et al 2012;Liao et al 2015). Although this phenomenon was firstly described for bacterial biofilms, yeast infections caused by biofilms, including Candida and Trichosporon, were also reported to exhibit high drug resistance (Al-Fattani and Douglas 2006;d'Enfert 2006;Di Bonaventura et al 2006).…”

Section: Introductionmentioning

confidence: 98%

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Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

et al. 2017

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Emerging resistance of microbial cells towards antibiotics or disinfectants leads to an increase of nosocomial diseases, often biofilm related, and gives rise to the need for research for new antimicrobial substances. These studies are often focused on substances that are able to support the efficacy of currently used antimicrobials, reduce their required dosage and reduce the probability of resistance development. This work brings new insights into the in vitro interaction of rhamnolipid biosurfactant and amphotericin B against Candida sp. or Trichosporon sp.. The minimum inhibitory concentrations and fractional inhibitory concentration indexes for both planktonic and biofilm cells were determined by the chequerboard microdilution method. Combination effect of rhamnolipid and amphotericin B was observed for both yeasts. Synergy, defined as a fractional inhibitory concentration (FICi) index of 0.50, was observed in both planktonic and biofilm cells of Trichosporon cutaneum. For C. parapsilosis, synergistic effect (FICi 0.5) was observed for planktonic cells, and additive effect (FICi 0.8) for biofilm cells. The influence of concentrations established as FICi on biofilm formation and susceptibility was studied by light microscopy. The highest inhibition (90% colonized area reduction) of initial adhesion of C. parapsilosis was observed when biofilm FIC was applied.

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Section: Effect Of Rhamnolipid and Amphotericin B On Biofilm Formationsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 98%

Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

et al. 2017

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“…C. albicans biofilms were fixed with 2.5 % glutaraldehyde in 0.15 M of sodium cacodylate buffer with Alcian blue (0.1 %), incubated overnight at 4 °C, dehydrated with an ascending ethanol series, dried with hexamethyldisilazane (Polysciences Europe, Hirschberg an der Bergstraße, Germany), coated with 10 nm of gold (Emitech Q150T, Quorum Technologies Ltd, Laughton, UK) and observed with an FEI Inspect S50 scanning electron microscope (Brno, Czech Republic) operating in high-vacuum mode at 15 kV [17].…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein

Sá

Silva

Neto

et al. 2020

Journal of Medical Microbiology

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This study evaluated the effect of etomidate against biofilms of Candida spp. and analysed through molecular docking the interaction of this drug with ALS3, an important protein for fungal adhesion. Three fluconazole-resistant fungi were used: Candida albicans, Candida parapsilosis and Candida tropicalis. Growing biofilms were exposed to etomidate at 31.25–500 µg ml−1. Then, an ALS3 adhesive protein from C. albicans was analysed through a molecular mapping technique, composed of a sequence of algorithms to perform molecular mapping simulation based on classic force field theory. Etomidate showed antifungal activity against growing biofilms of resistant C. albicans, C. parapsilosis and C. tropicalis at all concentrations used in the study. The etomidate coupling analysis revealed three interactions with the residues of interest compared to hepta-threonine, which remained at the ALS3 site. In addition, etomidate decreased the expression of mannoproteins on the surface of C. albicans. These results revealed that etomidate inhibited the growth of biofilms.

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“…fibuligera is a nonpathogenic yeast which has been employed as a comparator in C. tropicalis gut inflammation studies (17, 18). Next, we determined the ability of the designed probiotic to prevent and treat PMB formed by C.…”

Section: Introductionmentioning

confidence: 99%

Effects of a Novel Probiotic Combination on Pathogenic Bacterial-Fungal Polymicrobial Biofilms

Hager

Isham

Schrom

et al. 2019

mBio

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Dysbiosis of the gut microbiome has been implicated in inflammatory bowel diseases. We have shown that levels ofCandida tropicalis, along with those ofEscherichia coliandSerratia marcescens, are significantly elevated in Crohn’s disease (CD) patients. Here, we evaluated the ability of a novel probiotic to prevent and treat polymicrobial biofilms (PMB) formed byC. tropicaliswithE. coliandS. marcescens. SinceCandida albicanshas been reported to be elevated in CD patients, we investigated the interactions ofC. albicanswith these bacterial species in biofilm formation. We determined whether the interaction betweenCandidaspp. and bacteria is specific by usingTrichosporon inkinandSaccharomyces fibuligeraas comparators. Additionally, the effects of probiotics onC. albicansgermination and biofilm formation were determined. To determine the ability of the probiotic to prevent or treat mature biofilms, probiotic filtrate was added to the PMB at early (prevention) and mature (treatment) phases. Biofilm thickness and architecture were assessed by confocal scanning laser microscopy. The effects of the probiotic on germination were evaluated in the presence of serum. Exposure ofC. tropicalisPMB to probiotic filtrate reduced biofilm matrix, decreased thickness, and inhibited hyphal formation. We showed thatC. albicansorC. tropicalisformed significantly thicker PMB than control biofilms, indicating that this interaction isCandidaspecific. Treatment with probiotic filtrate inhibitedC. albicansgermination and prevented/treatedC. albicansPMB. The designed probiotic may have utility in the management of biofilm-associated gastrointestinal diseases such as Crohn’s and colorectal cancer.IMPORTANCEThe effects of diversity of the gut microbiome on inflammation have centered mainly on bacterial flora. Recent research has implicated fungal species and their interactions with other organisms in the inflammatory process. New ways to restore microbial balance in the gut are being explored. Our goal was to identify beneficial probiotic strains that would antagonize these fungal and bacterial pathogens that are elevated in the inflamed gut, and which also have antibiofilm activity. Fungus-bacterium correlation analysis allowed us to identify candidate probiotic species that can antagonize microbial pathogens, which we subsequently incorporated into a novel probiotic formulation. Amylase, which is known to have some antibiofilm activity, was also added to the probiotic mixture. This novel probiotic may have utility for the management of inflammatory bowel diseases by disrupting polymicrobial biofilm formation.

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Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals

Cited by 34 publications

References 50 publications

Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein

Effects of a Novel Probiotic Combination on Pathogenic Bacterial-Fungal Polymicrobial Biofilms

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