Genetic Diversity Analysis of Medicinal Plant Cassia alata in Andaman Island through ISSR Markers

Molecular characterization of Asplenium nidus L. – A potential ornamental fern species from Andaman

“…The level of polymorphism in A. nidus as number of polymorphic allele per primer and percent polymorphism ranged from1to 5 alleles and 33.3 to 83.3 %, respectively among twelve accessions. This vast range in percent polymorphism obtained in the present study proved the discriminatory power of ISSR molecular markers for distinguishing different cultivars reported in earlier literatures (Powell et al, 1996;Joshi et al, 2000;Ahmad et al 2010;Abirami et al, 2016).The present study revealed the ISSR marker, UBC 807 with higher number of polymorphic alleles and percent polymorphism; whereas, the marker, ISSR 23 with the lowest number of polymorphic alleles and percent polymorphism( Table 2). The number of alleles and polymorphic nature of these ISSR markers indicated their robustness in fingerprinting of species.…”

“…Generally, the relationships among accessions in the cluster could be attributed to their diversity, geographical locations and also due to exchange of plant genetic resources among farmers within and between the different regions of the Island. It has been shown that ISSR molecular markers have sufficient discriminatory power to distinguish different cultivars (Powell et al, 1996;Joshi et al, 2000;Ahmad et al 2010;Abirami et al 2016) and reliably used for quantification of genetic diversity and relationships, species identification and evaluation of conservation status (Abirami et al, 2018). The variability within a species is low due to less degree of gene flow in population through random mating without barrier (Ahmad et al, 2010).…”