Genetic Diversity Among Brazilian Cultivars and Landraces of Tomato Lycopersicon Esculentum Mill. Revealed by RAPD Markers

Abstract

Random amplified polymorphic DNA markers (RAPD) were used to estimate the variability of 35 tomato accessions (Lycopersicon esculentum Mill.). A total of 257 reproducibly scorable bands were obtained from 20 primers, 78.6% of which were polymorphic. The percentage distribution of RAPD markers shows a bimodal distribution, and the frequency of rare alleles is similar in commercial and landrace accessions. Genetic distances among accessions were calculated and a dendrogram showing the genetic relationships among them was constructed allowing for the separation of four groups. Twenty out of 23 Brazilian landraces fell within one group, whereas commercial cultivars were distributed in the four groups. AMOVA analysis of RAPD data showed that, despite the high within Brazilian landraces and commercial cultivars variation, these two groups are significantly different, indicating that landraces can be a source of variation for breeding programs.

Caracterização química e física de frutos de diferentes acessos de tomateiro em casa de vegetação

“…Mesmo as variedades tradicionalmente brasileiras são originadas de sementes trazidas por imigrantes europeus, embora alguns materiais selvagens sejam encontrados eventualmente (CARELLI et al, 2006). Segundo Ferreira (2004), a preferência do consumidor é determinada por características como tamanho, cor, forma, firmeza da polpa e casca e aparência geral do fruto.…”

Identification and Genetic Diversity of Korean Tomato Cultivars by RAPD Markers

“…The lack of polymorphism has also been reported in other self-pollinated crops such as soybean, potato, and ground nut [7,11,20]. Such insufficient variability in DNA polymorphism makes it difficult to construct a genetic map, if using populations derived from across between cultivars [2]. A low level DNA polymorphism in cultivated tomato was also reported using AFLP (amplified fragment length polymorphisms) and SSR (simple sequence repeats) primer pairs [14].…”

“…The progression has greatly increased the responsibility and visibility of those charged with long-term conservation and the use of plant genetic resources [2]. It is very important to define identity, purity, and stability of varieties for breeder's rights protection as well as for an effective seed quality control program [11,13].…”

Genetic diversity among tomato’s subsamples for pre-breeding

“…However, in spite of their great potential as a source of variability, the lack of information about their origin, genealogy, agronomic performance and genetic background has hindered the use of these varieties in breeding programs (Carelli et al 2006), causing a narrowing of the genetic base of the new cultivars.…”

“…Therefore, studies aiming at estimating the diversity of several plant species have been reported for several cultures (Carvalho and Quesenberry 2009, Serqueira-Silva et al 2009, Mazzucato et al 2008, Nick et al 2008, Nizio et al 2008, Rocha et al 2008, Carelli et al 2006, Karasawa et al 2005, Silva et al 2001.…”