Genetic divergence in Tetragonisca angustula Latreille, 1811 (Hymenoptera, Meliponinae, Trigonini) based on rapd markers

Oliveira, Rosana de Cassia; Nunes, Francis de Morais Franco; Campos, Ana Paula Soraggi; Vasconcelos, Soraya Matos de; Roubik, David W.; Goulart, Luiz Ricardo; Kerr, Warwick Estevam

doi:10.1590/s1415-47572004000200009

Cited by 27 publications

(23 citation statements)

References 9 publications

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“…Studies reporting on the detection of species-specific patterns that permit distinguishing closely related organisms using RAPD (Waldschmidt et al, 2000;Oliveira et al, 2004) or microsatellite markers (Estoup et al, 1995;Haavie et al, 2000;Dhuyvetter et al, 2004) are also well documented.…”

Section: Discussionmentioning

confidence: 99%

Genetic divergence between populations of the stingless bee uruçu amarela (Melipona rufiventris group, Hymenoptera, Meliponini): is there a new Melipona species in the Brazilian state of Minas Gerais?

et al. 2007

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Allozyme, microsatellite and random amplified polymorphic DNA (RAPD) molecular markers were used to investigate the within and between population genetic variability and between population genetic differentiation of the Brazilian stingless bee uruçu amarela (nominally Melipona rufiventris Lepeletier, 1836) present in savanna and Atlantic forest habitats of the Brazilian state of Minas Gerais (MG). We found low levels of within population variability, although there were a large number of private alleles that specifically characterized these populations. The F ST values indicated a high level of genetic diversity between populations. Analysis of molecular variance (AMOVA) showed a high degree of population differentiation between the savanna and Atlantic forest habitats, confirmed by population pairwise F ST data. Principal coordinates analysis and unweighted pair-group method using an arithmetic average (UPGMA) dendrograms also confirmed that in Minas Gerais the savanna populations (M. rufiventris) were genetically distinct from those present in the Atlantic forest (M. mondury). In addition, populations from locations near the towns of Dom Bosco and Brasilândia de Minas were genetically different from those collected in other localities in the savanna. Our data indicate that populations of uruçu amarela found in the savanna and Atlantic forest habitats of Minas Gerais state should be treated separately for conservation purposes and that special attention should be given to the populations found in the region of Dom Bosco and Brasilândia de Minas until their taxonomic status is clarified.

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Section: Discussionmentioning

confidence: 99%

Genetic divergence between populations of the stingless bee uruçu amarela (Melipona rufiventris group, Hymenoptera, Meliponini): is there a new Melipona species in the Brazilian state of Minas Gerais?

et al. 2007

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“…Relatively few genetic studies have investigated bee species within other genera, such as Plebeia remota (Francisco et al, 2001), Eufrisea violacea (Sofia et al, 2005), Macrotera portalis (Danforth et al, 2003), Tetragonisca angustula (Oliveira et al, 2004), Partamona helleri (Brito and Arias, 2010), Partamona seridoensis (Fernandes et al, 2012), Melipona subnitida (Cruz et al, 2006), and Euglossa fimbriata (Suzuki et al, 2010). Some of these studies have used PCR-RFLP (Francisco and Arias, 2010;Brito and Arias, 2010;Suzuki et al, 2010) revealing interesting information that has allowed researchers to distinguish populations.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA variability in populations of Centris aenea (Hymenoptera, Apidae), a crop-pollinating bee in Brazil

Ferreira

Aguiar²,

Oliveira³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Centris spp are oil-collecting solitary bees that are valuable pollinators of crops such as Brazil nut, cashew, and acerola. We investigated the genetic variability of populations of C. aenea in the northeastern region of Brazil. Total DNA was extracted from 59 individuals from 6 locations in the States of Pernambuco and Bahia and a 600-650-bp fragment of the mitochondrial COI/COII region amplified by PCR, followed by digestion with the restriction enzymes DraI and SspI. PCR-RFLP analysis revealed eight different haplotypes among the populations. Haplotype A1, revealed by DraI, was the most frequent (50%), and haplotypes A3 and A4 were exclusive to Feira de Santana, Bahia and Morro do Chapéu, Bahia, respectively. Among the haplotypes revealed by SspI, B2 was the most frequent (37%) and B3 was exclusive to Feira de Santana. This information revealing high haplotype diversity will be useful for developing management strategies for Centris, especially because of increasing interest in the rearing and/ or relocation of these bees for crop pollination.

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“…Utilizando a mesma metodologia, Paiva (2001) avaliou efeitos de obstáculos naturais na estruturação de populações em espécies de peixes. Gimenez et al (2003) analisaram subespécies de suínos e seus híbridos, e Oliveira et al (2004) determinaram as distâncias genéticas entre populações de abelhas de 25 localidades, de três países da América Latina.…”

Section: Introductionunclassified

Variabilidade genética em búfalos estimada por marcadores RAPD

Albuquerque

Egito

Marques

et al. 2006

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi caracterizar, por meio de marcadores RAPD, dois grupos genéticos de búfalos, Carabao e tipo Baio, que estão sendo conservados in situ, assim como verificar as relações genéticas entre eles e os outros três grupos genéticos de búfalos existentes no Brasil, Murrah, Jafarabadi e Mediterrâneo, considerados raças comerciais. Foram estudados 48 animais de cada grupo, com exceção dos grupos Murrah e Mediterrâneo, com 47 e 42 animais, respectivamente, compreendendo um total de 233 animais. Os 21 iniciadores polimórficos geraram 98 marcadores. A variabilidade genética entre e dentro dos grupos foi estimada em 26,5 e 73,5%, respectivamente, sugerindo divergência significativa entre os cinco grupos genéticos. Na análise entre pares de grupos, foi verificado que a maior e a menor divergência estavam em torno de 40 e 18%, quando se compararam os grupos Carabao x Mediterrâneo e Murrah x Jafarabadi, respectivamente. Entre os grupos Baio e Murrah, a análise revelou divergência genética de 20,42%, indicando que esses grupos são distintos. Os cinco grupos são geneticamente distintos, o que reforça a necessidade de conservação dos grupos genéti-cos Carabao e Baio, ameaçados de extinção no Brasil.Termos para indexação: Bubalus bubalis, caracterização genética, marcadores moleculares. Genetic variability of buffaloes estimated by RAPD markersAbstract -The objective of this work was to characterize genetically, using RAPD markers, two genetic groups of buffalos, Carabao and Baio, which are being conserved in situ, as well as to verify the genetic relationship among them and the other three genetic groups of buffalos raised in Brazil, considered as commercial breeds: Murrah, Jaffarabadi and Mediterrâneo. Forty eight animals of each group were studied, with the exception of the Murrah and Mediterrâneo, in which 47 and 42 animals, respectively, were sampled, comprising a total of 233 animals. The 21 polymorphic primers produced 98 markers. Genetic variability within and between groups was estimated in 26.5 and 73.5%, respectively, suggesting a significant divergence among groups. On the analysis between pairs of groups, the divergence was about 40 and 18%, respectively, when the Carabao x Mediterranâneo and the Murrah x Jaffarabadi were compared. The estimated genetic divergence between Baio and Murrah groups was 20.42%, indicating that they are distinct groups. The five genetic groups are genetically distinct, indicating the need to conserve the Carabao and Baio, two genetic groups in danger of extinction in Brazil.

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Genetic divergence in Tetragonisca angustula Latreille, 1811 (Hymenoptera, Meliponinae, Trigonini) based on rapd markers

Cited by 27 publications

References 9 publications

Genetic divergence between populations of the stingless bee uruçu amarela (Melipona rufiventris group, Hymenoptera, Meliponini): is there a new Melipona species in the Brazilian state of Minas Gerais?

Genetic divergence between populations of the stingless bee uruçu amarela (Melipona rufiventris group, Hymenoptera, Meliponini): is there a new Melipona species in the Brazilian state of Minas Gerais?

Mitochondrial DNA variability in populations of Centris aenea (Hymenoptera, Apidae), a crop-pollinating bee in Brazil

Variabilidade genética em búfalos estimada por marcadores RAPD

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