Genetic divergence among and within Colletotrichum lindemuthianum races assessed by RAPD

Talamini, Viviane; Souza, Elaine Aparecida de; Pozza, Edson Ampélio; Silva, Gilvan Ferreira da; Ishikawa, Francine Hiromi; Júnior, Osnil A. Camargo

doi:10.1590/s0100-41582006000600002

Cited by 13 publications

(18 citation statements)

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“…Although some authors (Sicard et al 1997;González 1998) have described a tendency of isolates to cluster by region of origin, Mahuku & Riascos (2004) reported no separation of groups according to host genetic pool or geographic region. Similar results have been obtained by Fabre et al (1995), Balardin et al (1997Balardin et al ( , 1999, Talamini et al (2006) and Silva et al (2007).…”

Section: Discussionsupporting

confidence: 90%

“…In these investigations, races from various countries were evaluated, and when several isolates per race were analyzed they generally clustered in different genetic similarity groups showing the existence of within-race variability. Additionally, Alzate-Marin et al (2001), Talamini et al (2006) and Silva et al (2007) have observed within-race variation in Brazilian isolates. Based on the clustering of similarity estimates for anastomosis, a high number of anastomosis groups were observed within C. lindemuthianum race 65, however, of the 13 isolates investigated only three (LV58, LV61 and LV73) belonged to the same anastomosis group.…”

Section: Discussionmentioning

confidence: 97%

“…The use of molecular techniques for the detection of genetic variability offers an alternative solution. Molecular studies have shown that there is large within-race variability in C. lindemuthianum (Silva et al 2007), although a direct relationship between molecular genotype and races identified by inoculation in differential cultivars has not been observed (Otoya et al 1995;Balardin et al 1997;Alzate-Marin et al 2001;Talamini et al 2006). Anastomosis, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil

2008

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Colletotrichum lindemuthianum, the causal agent of anthracnose in the common bean (Phaseolus vulgaris), presents a wide genetic and pathogenic variability that gives rise to complications in the development of resistant bean cultivars. The aim of this study was to identify the variability within race 65 of C. lindemuthianum, the race most commonly encountered in Brazil, through randomly amplified polymorphic DNA (RAPD) and anastomosis analyses. Thirteen isolates of race 65, collected in different years and from various host cultivars located in diverse areas of the state of Minas Gerais, Brazil, were investigated. Twenty-four RAPD primers were employed and 83 polymorphic bands amplified. Genetic similarities were estimated from the Sorensen-Dice coefficient and ranged from 0.54 to 0.82. The dendrogram obtained by cluster analysis classified the isolates into 11 separate groups. For the purposes of the analysis of anastomosis, isolates were considered to be compatible when the fusion of hyphae from different isolates could be observed. The proportion of compatible reactions for each isolate was estimated and similarity estimates, based on the Russel & Rao coefficient, ranged from 0.28 to 0.85. Isolates were classified into 11 anastomosis groups, 10 of which were formed by only one isolate. Although isolates LV61, LV73 and LV58 were classified in the same anastomosis group, they were genetically distinct according to RAPD analysis. Results from both RAPD and anastomosis analyses revealed great variability within C. lindemuthianum race 65.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil

2008

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“…On the other hand, the isolate 4 from the State of Minas Gerais revealed the presence of one SNP in the ITS1 region and one in the ITS2 region, both are different from the SNPs observed in the other isolates. Studies conducted by Talamini et al (2006) noted the presence of molecular variability between and within isolates belonging to race 65 from the State of Minas Gerais.…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

“…The common bean anthracnose is one of the main fungal diseases affecting this crop, especially if the environmental conditions favor the pathogen's development, leading to yield losses of up to 100% when susceptible cultivars are used (Talamini et al, 2006; Damasceno e Silva, Souza, & Ishikawa, 2007 pathogen and is therefore widely distributed in common bean-producing regions (Bianchini, Maringoni, & Carneiro, 2005). Anthracnose occurs more severely in places where relative humidity conditions above 91% and temperatures ranging from 18° and 22°C predominate.…”

Section: Introductionmentioning

confidence: 99%

<b>Characterization of race 65 of <i>Colletotrichum lindemuthianum</i> by sequencing ITS regions

et al. 2016

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ABSTRACT. The present work aimed characterize isolates of C. lindemuthianum race 65 from different regions in Brazil by ITS sequencing. A total of 17 isolates of race 65, collected in the states of Mato Grosso, Minas Gerais, Paraná, Santa Catarina and São Paulo, were studied. Analysis of the sequences of isolates 8, 9, 12, 14 and 15 revealed the presence of two single nucleotide polymorphisms (SNPs) in the ITS1 region at the same positions. These isolates, when analyzed together with the sequence of isolate 17, revealed a SNP in the ITS2 region. The highest genetic dissimilarity, observed between isolates 11 and 3 and between isolates 11 and 10, was 0.772. In turn, isolates 7 and 2 were the most similar, with a value of 0.002 for genetic distance. The phylogenetic tree obtained based on the sequences of the ITS1 and ITS2 regions revealed the formation of two groups, one with a subgroup. The results reveal high molecular variability among isolates of race 65 of C. lindemuthianum.Keywords: anthracnose, genetic variability, pathogens, dissimilarity.Caracterização da raça 65 de Colletotrichum lindemuthianum utilizando sequenciamento das regiões ITS RESUMO. O presente trabalho teve como objetivo caracterizar isolados de C. lindemuthianum da raça 65 provenientes de diversas regiões do Brasil, por meio de sequenciamento de regiões ITS. Um total de 17 isolados da raça 65, coletados nos estados do Mato Grosso, Minas Gerais, Paraná, Santa Catarina e São Paulo, foram estudados. As análises das sequências dos isolados 8, 9, 12, 14 e 15 revelaram a presença de dois SNPs na região ITS1 nas mesmas posições. Estes mesmos isolados quando analisados juntamente com a sequência do isolado 17 apresentaram um SNP na região ITS2. A maior dissimilaridade genética foi de 0,772 observada entre os isolados 11 e 3 e entre os isolados 11 e 10. Por sua vez, os isolados 7 e 2 foram os mais similares, com valor de distância genética de 0,002. A árvore filogenética obtida com base nas sequências das regiões ITS1 e ITS2 revelou a formação de dois grupos, sendo um com a divisão de um subgrupo. Estes resultados revelam uma elevada variabilidade molecular entre isolados da raça 65 de C. lindemuthianum.

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Development of new molecular markers for the Colletotrichum genus using RetroCl1 sequences

Santos

Queiroz

Santana

et al. 2011

World J Microbiol Biotechnol

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A nonautonomous element of 624 bp, called RetroCl1 (Retroelement Colletotrichum lindemuthianum 1), was identified in the plant pathogenic fungus Colletotrichum lindemuthianum. RetroCl1 contains terminal direct repeats (223 bp) that are surrounded by CTAGT sequences. It has a short internal domain of 178 bp and shows characteristics of terminal-repeat retrotransposon in miniature (TRIM) family. We used RetroCl1 sequence to develop molecular markers for the Colletotrichum genus. IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism) markers were used to analyze the genetic diversity of C. lindemuthianum. Fifty-four isolates belonging to different races were used. A total of 45 loci were amplified. The Nei index showed significant differences among the populations divided according to race, indicating that they are structured according to pathotype. No clear correlation between IRAP and REMAP markers with pathogenic characterization was found. C. lindemuthianum has high genetic diversity, and the analysis of molecular variance showed that 51% of variability is found among the populations of different races. The markers were also tested in different Colletotrichum species. In every case, multiple bands were amplified, indicating that these markers can be successfully used in different species belonging to the Colletotrichum genus.

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Genetic divergence among and within Colletotrichum lindemuthianum races assessed by RAPD

Cited by 13 publications

References 13 publications

Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil

Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil

<b>Characterization of race 65 of <i>Colletotrichum lindemuthianum</i> by sequencing ITS regions

Development of new molecular markers for the Colletotrichum genus using RetroCl1 sequences

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