Development of new molecular markers for the Colletotrichum genus using RetroCl1 sequences

Abstract

A nonautonomous element of 624 bp, called RetroCl1 (Retroelement Colletotrichum lindemuthianum 1), was identified in the plant pathogenic fungus Colletotrichum lindemuthianum. RetroCl1 contains terminal direct repeats (223 bp) that are surrounded by CTAGT sequences. It has a short internal domain of 178 bp and shows characteristics of terminal-repeat retrotransposon in miniature (TRIM) family. We used RetroCl1 sequence to develop molecular markers for the Colletotrichum genus. IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism) markers were used to analyze the genetic diversity of C. lindemuthianum. Fifty-four isolates belonging to different races were used. A total of 45 loci were amplified. The Nei index showed significant differences among the populations divided according to race, indicating that they are structured according to pathotype. No clear correlation between IRAP and REMAP markers with pathogenic characterization was found. C. lindemuthianum has high genetic diversity, and the analysis of molecular variance showed that 51% of variability is found among the populations of different races. The markers were also tested in different Colletotrichum species. In every case, multiple bands were amplified, indicating that these markers can be successfully used in different species belonging to the Colletotrichum genus.

<b>Classification of <i>Colletotrichum lindemuthianum</i> races in differential cultivars of common bean

“…Moreover, they report the possibility of CAT formation between C. lindemuthianum and G. cingulata, increasing the pathogen variability (Barcelos, Pinto, Vaillancourt, & Souza, 2014). The genetic diversity of C. lindemuthianum was also reported by molecular markers using IRAP (Interretrotransposon Amplified Polymorphism) and Remap (retrotransposon Microsatellite), indicating high variability between groups of the pathogen and effectiveness in perform genetic diversity analysis (Santos et al, 2012;Gonzaga, Costa, Santos, Araujo, & Queiroz, 2014).…”

Genetic variability of Fusarium solani and Fusarium oxysporum f. sp. passiflorae isolates from Pantanal, Amazon and Cerrado biomes of Mato Grosso, Brazil

“…The primers used in this study Table 1. Primers used in the analysis of IRAP and ISSR markers (Longato and Bonfante, 1997;Santos et al, 2012).…”

Draft Genome Sequences of Two Isolates of Colletotrichum lindemuthianum , the Causal Agent of Anthracnose in Common Beans

“…Under favorable conditions of humidity and temperature, in extreme cases, anthracnose can cause death of the plant (4). C. lindemuthianum has a high genetic variability, manifested by the presence of many physiologically diverse races (5). This high genetic variability is one of the main limiting factors to combating this pathogen, because it prevents the long-term use of resistant cultivars (6).…”