Genetic dissection reveals effects of interaction between high-molecular-weight glutenin subunits and waxy alleles on dough-mixing properties in common wheat

Abstract

The glutenin and waxy loci of wheat are important determinants of dough quality. This study was conducted to evaluate the effects of high-molecular-weight glutenin (HMW-GS) and waxy alleles on dough-mixing properties. Molecular mapping was used to investigate these effects on Mixograph properties in a population of 290 (Nuomai1 x Gaocheng8901) recombinant inbred lines (RILs) from three environments in the harvest years 2008, 2009 and 2011. The results indicated the following: (i) the Glu-A1 and Glu-D1 loci have greater impacts on Mixograph properties compared to the Wx-1 loci and the effects of Glu-D1d and Glu-D1h on dough mixing are better than those of Glu-D1f and Glu-D1new1 in this population; (ii) the interactions between the Glu-1 and Wx-1 loci affected some traits, especially the midline peak value (MPV), and the lack of Wx-B1 or Wx-D1 led to increased MPV for all types of Glu-1 loci; and (iii) 30 quantitative-trait loci (QTL) over nine wheat chromosomes were identified with ICIM analysis based on the genetic map of 498 loci. Eight major QTL and 16 QTL in the Glu-1 loci from the three environments were found. The major QTL clusters were associated with the Glu-1 loci, and also were found in two regions on chromosome 3B and one region on chromosome 6A, which is one of the novel chromosome regions influencing dough-mixing strength. The two QTL for MPV are located around Wx-B1 on chromosome 4A. QMPT-1D.1, QMPI-1D.1 and Q8MW-1D.1 were stable in different environments and could potentially be used in molecular marker-assisted breeding.

Genome-Wide QTL Mapping for Wheat Processing Quality Parameters in a Gaocheng 8901/Zhoumai 16 Recombinant Inbred Line Population

“…Although 119 additive QTLs were detected for dough rheological and starch pasting properties, 50 of them were grouped into 11 clusters. QTL clusters for dough strength on chromosomes 1AL, 1BL, and 1DL detected in the present study were associated with HMW-GSs, consistent with previous reports (Huang et al, 2006 ; Deng et al, 2013 ). The interval 24.9–34.2 cM on chromosome 2BS is a QTL cluster impacting MPT, MTxW, WA, ST and C2, which coincides with their significant phenotypic correlations.…”

“…A similar co-localized region for dough development time, stability and softening was also located on chromosome 2B (Maphosa et al, 2015 ). A QTL cluster for MPV, MPW and PTI between BS00072151_51 and GENE-1617_131 , positioned in the interval of 61.3–81.9 cM on chromosome 3B, is likely to be in the same region for MPT, MPI, and MTxW reported by Deng et al ( 2013 ). Another QTL cluster for MPT, MTxW, DT, ST, C2, and C3 identified in the interval 35.8–63.1 cM on chromosome 4B, is likely the same as a QTL cluster for Mixograph midline peak time, midline tail width, envelope peak energy and envelope peak time (Patil et al, 2009 ).…”

“…Although there are many reports on QTL for quality traits in wheat (Huang et al, 2006 ; McCartney et al, 2006 ; Sun et al, 2008 ; Patil et al, 2009 ; Zhao et al, 2009 ; Tsilo et al, 2011 ; Li et al, 2012 ; Deng et al, 2013 , 2015 ; Zheng et al, 2013 ; Prashant et al, 2015 ), few genes are identified in single QTL analysis. The main reason is that SSR markers previously used in QTL analysis have insufficient density and are often in non-coding regions.…”

“…Dough strength and starch pasting characteristics are quantitative traits controlled by multiple genes. Several QTLs for Mixograph (Huang et al, 2006 ; McCartney et al, 2006 ; Patil et al, 2009 ; Tsilo et al, 2011 ; Li et al, 2012 ; Deng et al, 2013 ; Zheng et al, 2013 ; Prashant et al, 2015 ) and RVA parameters (McCartney et al, 2006 ; Sun et al, 2008 ; Zhao et al, 2009 ; Deng et al, 2015 ) were reported using different populations. However, these reports were primarily based on simple sequence repeat (SSR) markers, resulting in relatively large genetic distances defining QTL and markers due to the limited numbers of such markers, which leads to difficulty for gene cloning based on QTL analysis and marker-assisted selection (MAS) in wheat breeding.…”

Identification of chromosomal regions in the genetic control of quality traits in durum wheat (Triticumturgidum L.) from the Fertile Crescent

“…Conti et al (2011) identified 3 QTLs on chromosome 1A. Deng et al (2013) indicated that the Glu-A1 locus has greater impacts on mixograph properties associated with dough mixing. Jin et al (2016) reported many QTLs linked with Glu-A1-HMW for the mixograph midline peak value, midline peak width, and mixograph development time, and the most important QTLs causing significant variations in the studied traits were located on chromosomes 1BL, 6AL, 6BL, and 7AS.…”