Genetic differentiation ofOmpok bimaculatus(Teleostei: Siluridae) population based on mtDNA cytochromebgene

Abstract

Ompok bimaculatus commonly called as Indian butter catfish is a commercially important food fish and distributed throughout India and Southeast Asia. Genetic differentiation of O. bimaculatus was investigated using cytochrome b (cyt b) gene of three different riverine populations. A total of 37 sequences of cyt b gene (1118 bp) were generated from O. bimaculatus. The mitochondrial DNA analysis revealed that O. bimaculatus has relatively high genetic diversity. The estimated haplotype and nucleotide diversity were 0.063 and 0.005, respectively. The analysis of molecular variance indicated 73.53% genetic variability between the populations and 26.47% genetic variation within population. The understanding of genetic differentiation of O. bimaculatus populations will play a key role in conservation and management of the important endangered fish species.

Untitled

“…In this study we have not yet determined genetic variation of O. bimaculatus, but previous studies have revealed genetic differentiation levels suggesting very limited gene flow among populations (Malakar et al, 2013). Biological effects and/ or unsustainable fishing practices may lead to a modification of their reproductive strategies: decreasing their L50 and increasing their reproduction effort (Kovacic, 2004;Perera-Garcia et al, 2011).…”

“…The differences in reproductive potential of O. bimaculatus among different populations may reflect environmentally induced phenotypic plasticity and /or genetic variations (Gueye et al, 2012). In a recent study, Malakar et al (2013) reported relatively high genetic diversity of O. bimaculatus using cyt-b gene of three different riverine populations of O. bimaculatus. It can be predicted, therefore, that the O bimaculatus populations studied here and showing high variations in reproductive traits are genetically distinct.…”

“…To date, information concerning the reproductive potential of butter catfish is very limited and despite its economic importance, no systematic studies have been done. Previous studies have established length weight relationship (Sivakami, 1987), condition factor (Parameshwaran et al, 1970;Qayyum and Qasim, 1965), spawning biology (Raizada et al, 2013;Rao and Karamchandani, 1986;Sridhar et al, 1998) and genetic characterization (Malakar et al, 2013). The life history of O. bimaculatus has earlier been described by many researchers (Chakrabarty et al, 2007;Choudhuri, 1962;Mukherjee and Das, 2002;Rao, 1981;Sarkar et al, 2005); however, no attempts have been made on comparative analysis of reproductive traits of fish from different wild population.…”

Short Communication: Molecular characteristics and phylogenetic relationships of silurid catfishes (Kryptopterus, Ompok and Phalacronotus) from the Kampar River, Indonesia, based on the cytochrome b gene

“…Low G values are commonly found in the mitochondrial DNA of the Iberian Barbels fishes (Doadrio et al 2002) and Glyptosternoid fishes (Peng et al 2004). This lower G value is also found in O. bimaculatus (Malakar et al 2013). The pattern of differences between A, T, C, and G can show genetic signs and characteristics of a species (Afreixo et al 2009).…”

“…The cytochrome b gene contains the position of codons that develop slowly and rapidly, as well as more intraspecific variations, especially in the third position of codons that can be used to identify stocks (Habib et al 2011). Therefore, this gene has been used for a diversity of systematic questions (Malakar et al 2013). In this study, the mitochondrial cytochrome b gene region (906 base pairs) was used for genetic markers of 14 individuals from four species from three genera of silurid catfishes in the Kampar River, Indonesia.…”