Genetic detection of Babesia bigemina from Mongolian cattle using apical membrane antigen-1 gene-based PCR assay

Sivakumar, Thillaiampalam; Altangerel, Khukhuu; Battsetseg, Badgar; Battur, Banzragch; AbouLaila, Mahmoud; Munkhjargal, Tserendorj; Yoshinari, Takeshi; Yokoyama, Naoki; Igarashi, Ikuo

doi:10.1016/j.vetpar.2012.01.008

Cited by 59 publications

(38 citation statements)

References 19 publications

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“…Sequence analyses confirmed that the AMA-1 PCR assay amplified only the B. ovata AMA-1 gene. All sequences (GenBank accession numbers AB703297, AB703298, and AB733631) were identical to each other and to the previously reported Miyake strain of B. ovata(AB634843) [7,11].…”

confidence: 57%

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A PCR Based Survey of Babesia ovata in Cattle from Various Asian, African and South American Countries

et al. 2013

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ABSTRACT. Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B. ovata infection in Japan, Mongolia, and Thailand.

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confidence: 57%

“…Our previous study suggested the possible presence of B. ovata in Mongolia, a country where the parasite has not been previously reported [11]. Therefore, it is important to accurately detect B. ovata in cattle populations and determine its prevalence in different regions across the world.…”

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confidence: 98%

A PCR Based Survey of Babesia ovata in Cattle from Various Asian, African and South American Countries

et al. 2013

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“…The PCR reaction mixtures and cycling conditions have been described in previous reports (Sivakumar et al, 2013b;Tattiyapong et al, 2014). The amplified gene fragments were gel-extracted and cloned as described previously (Sivakumar et al, 2012a). For each cloned product, three clones were picked and both DNA strands of each were sequenced.…”

Section: Pcr Detection Of B Bovismentioning

confidence: 99%

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

Yokoyama

Sivakumar

Tuvshintulga

et al. 2015

Infection, Genetics and Evolution

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The genes that encode merozoite surface antigens (MSAs) in Babesia bovis are genetically diverse. In this study, we analyzed the genetic diversity of B. bovis MSA-1, MSA-2b, and MSA-2c genes in Vietnamese cattle and water buffaloes. Blood DNA samples from 258 cattle and 49 water buffaloes reared in the Thua Thien Hue province of Vietnam were screened with a B. bovis-specific diagnostic PCR assay. The B. bovis-positive DNA samples (23 cattle and 16 water buffaloes) were then subjected to PCR assays to amplify the MSA-1, MSA-2b, and MSA-2c genes. Sequencing analyses showed that the Vietnamese MSA-1 and MSA-2b sequences are genetically diverse, whereas MSA-2c is relatively conserved. The nucleotide identity values for these MSA gene sequences were similar in the cattle and water buffaloes. Consistent with the sequencing data, the Vietnamese MSA-1 and MSA-2b sequences were dispersed across several clades in the corresponding phylogenetic trees, whereas the MSA-2c sequences occurred in a single clade. Cattle- and water-buffalo-derived sequences also often clustered together on the phylogenetic trees. The Vietnamese MSA-1, MSA-2b, and MSA-2c sequences were then screened for recombination with automated methods. Of the seven recombination events detected, five and two were associated with the MSA-2b and MSA-2c recombinant sequences, respectively, whereas no MSA-1 recombinants were detected among the sequences analyzed. Recombination between the sequences derived from cattle and water buffaloes was very common, and the resultant recombinant sequences were found in both host animals. These data indicate that the genetic diversity of the MSA sequences does not differ between cattle and water buffaloes in Vietnam. They also suggest that recombination between the B. bovis MSA sequences in both cattle and water buffaloes might contribute to the genetic variation in these genes in Vietnam.

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“…Japan from 10 sika deer [15], E. canis (supplied by Dr. S. Harrus, Israel), E. muris (strain NA1) [33], Ehrlichia sp. from I. ovatus (strain HF565; supplied by dr. H. Fujita, Japan), A. bovis [29], A. centrale [14], A. platys [13], A. marginale [27], Theileria orientalis, Babesia bovis and B. ovata [30]. Moreover, plasmid DNAs from 2 of the positive samples were used as templates for the serial dilution PcR assay (herein designated as plasmid-IP11 and plasmid-HD10).…”

Section: Samplesmentioning

confidence: 99%

Dual Presence of Anaplasma phagocytophilum and Its Closely Related Anaplasma sp. in Ixodid Ticks in Hokkaido, Japan, and Their Specific Molecular Detection

Ybañez

Matsumoto

Kishimoto

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRAcT. Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely related and potentially novel Anaplasma sp. in Japan was recently characterized. The aims of the study were to provide molecular evidence for the presence of these 2 species in Japan, and to develop a reliable PcR method based on the nucleotide differences within the citrate synthase (gltA) gene. DNA samples from 182 ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii and 13 I. ovatus) collected from 2 sites in Hokkaido, Japan, were screened for A. phagocytophilum and its closely related Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PcR, revealing a combined prevalence rate of 27.5% (50 samples). The positive samples were then used to evaluate a newly developed gltA-based nested PcR method. Selected positive samples were further characterized using the groEL gene for confirmation and phylogenetic analyses. Two groups of sequence results were obtained: those that had closer identities with (1) A. phagocytophilum (99.5-99.6% for 16S rRNA, 97.5% for gltA and 98.4% for groEL), and those that had closer identities with (2) Anaplasma sp. closely related to A. phagocytophilum in Japan (99.3% for 16S rRNA, 96.4-98.7% for gltA and 97.5-97.9% for groEL). The present study confirmed the distinct presence of A. phagocytophilum and its closely related Anaplasma sp. in Japan, and developed a new PcR detection method based on gltA that can distinguish the 2 organisms.

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Genetic detection of Babesia bigemina from Mongolian cattle using apical membrane antigen-1 gene-based PCR assay

Cited by 59 publications

References 19 publications

A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries

A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

Dual Presence of <i>Anaplasma phagocytophilum</i> and Its Closely Related <i>Anaplasma</i> sp. in Ixodid Ticks in Hokkaido, Japan, and Their Specific Molecular Detection

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