Genetic damage in multiple organs of acutely exercised rats

Abstract

The aim of this study was to investigate the effects of acute exercise on genomic damage in an animal model. Male adult Wistar rats were divided into the following groups: control and acute exercised (experimental). For this purpose, 15 animals were accustomed to running on a rodent treadmill for 15 min per day for 5 days (10-20 m min(-1); 08 grade). After 4 days at rest, active animals ran on the treadmill (22 m min(-1), 58 grade) till exhaustion. Cells from peripheral blood, liver, heart, and brain were collected after 0, 2, and 6 h after exercise. The results showed that acute exercise was able to induce genetic damage in peripheral blood cells after 2 and 6 h of exercise, whereas liver pointed out genetic damage for all periods evaluated. No genetic damage was induced either in brain or in heart cells. In conclusion, our results suggest that acute exercise could contribute to the genetic damage in peripheral blood and liver cells. It seems that liver is a sensitive organ to the genotoxic insult after acute exercise.

Alpha-Tocopherol May Protect Hepatocytes Against Oxidative Damage Induced by Endurance Training in Growing Organisms

“…Hepatocytes are not indifferent to the genotoxic effect of physical activity, which could lead to genetic instability [29]. In this study, no changes in 8-OHdG levels were observed in the liver.…”

“…Similar results were reported by Kinoshita and Tsuji [27] and by Wawrzyniak et al [16], who demonstrated that endurance exercise had little effect on hepatic DNA damage and that 8-OHdG levels did not differ significantly between training and non-training subjects. Pozzi et al reported significantly higher levels of DNA damage in hepatic tissue after strenuous exercise [29]. According to Radak et al, exercise training accelerates DNA repair [31].…”

Exercise preconditioning modulates genotoxicity induced by doxorubicin in multiple organs of rats

“…Because of its simplicity and sensitivity, the comet assay has gained fast acceptance as a genotoxicity assay . Our group has consistently demonstrated that the single‐cell gel (comet) assay is a useful tool for detecting DNA breaks in multiple organs and under different paradigms, such as medium‐term carcinogenesis assays, acute EXC, sleep deprivation, drug abuse, or even following exposure to xenobiotics present in the environment in vivo …”

Effects of CoQ10 supplementation and swimming training on exhaustive exercise-induced oxidative stress in rat heart

“…Asami et al (51) found that the 8-OHdG levels in the forced exercise rats were signifi cantly elevated as compared with those in the spontaneously exercised rats in heart and other tissues and they indicate that the intensity of exercise training is an important determinant of oxidative DNA damage. Conversely, other investigators reported that DNA damage in heart tissue was unaffected by either acute or chronic exercise (36,52). However, Ramirez-Tortosa et al (53) stated that CoQ 10 supplementation decreased DNA damage in peripheral blood lymphocytes.…”