Genetic Control of Wiring Specificity in the Fly Olfactory System

Hong, Weizhe; Luo, Liqun

doi:10.1534/genetics.113.154336

Cited by 99 publications

(106 citation statements)

References 66 publications

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“…The transcriptomes of closely related PN classes differed substantially during development (Figure 7F; Table S1), consistent with a recent report that closely related retinal cells have dozens of differentially expressed CSMs (Tan et al, 2015). A certain degree of redundancy can provide robustness to wiring precision (Hong and Luo, 2014), but creates challenges for dissecting genetic control of wiring specificity using single gene manipulation. The transcriptomes of identified PN classes can inform design of more precise experiments in which simultaneous manipulation of multiple genes through loss- and gain-of-function approaches allows experimental testing of the combinatorial TF and CSM codes.…”

Section: Discussionmentioning

confidence: 99%

Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-Cell RNA Sequencing

Horns

et al. 2017

Cell

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243

189

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Summary The definition of neuronal type and how this relates to the transcriptome are open questions. Drosophila olfactory projection neurons (PNs) are among the best-characterized neuronal types: different PN classes target dendrites to distinct olfactory glomeruli, while PNs of the same class exhibit indistinguishable anatomical and physiological properties. Using single-cell RNA-sequencing, we comprehensively characterized the transcriptomes of most PN classes and unequivocally mapped transcriptomes to specific olfactory function for 6 classes. Transcriptomes of closely related PN classes exhibit the largest differences during circuit assembly but become indistinguishable in adults, suggesting that neuronal subtype diversity peaks during development. Transcription factors and cell-surface molecules are the most differentially expressed genes between classes and are highly informative in encoding cell identity, enabling us to identify a new lineage-specific transcription factor that instructs PN dendrite targeting. These findings establish that neuronal transcriptomic identity corresponds with anatomical and physiological identity defined by connectivity and function.

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Section: Discussionmentioning

confidence: 99%

Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-Cell RNA Sequencing

Horns

et al. 2017

Cell

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243

189

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“…In the Drosophila olfactory system, wiring specificity of OSNs and projection neurons (a Drosophila equivalent of mitral/tufted cells) are genetically pre-determined and precise matching between OSNs and projection neurons is mediated by various guidance molecules, including semaphorins, ephrins, and Teneurins [35]. These mechanisms ensure innate stereotyped behaviors to odors.…”

Section: Establishment Of a Glomerular Circuitrymentioning

confidence: 99%

Construction of functional neuronal circuitry in the olfactory bulb

Imai

2014

Seminars in Cell & Developmental Biology

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Recent studies using molecular genetics, electrophysiology, in vivo imaging, and behavioral analyses have elucidated detailed connectivity and function of the mammalian olfactory circuits. The olfactory bulb is the first relay station of olfactory perception in the brain, but it is more than a simple relay: olfactory information is dynamically tuned by local olfactory bulb circuits and converted to spatiotemporal neural code for higher-order information processing. Because the olfactory bulb processes ∼1000 discrete input channels from different odorant receptors, it serves as a good model to study neuronal wiring specificity, from both functional and developmental aspects. This review summarizes our current understanding of the olfactory bulb circuitry from functional standpoint and discusses important future studies with particular focus on its development and plasticity.

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“…1A;Jefferis and Hummel 2006;Hong and Luo 2014;Sakuma et al 2014). The primary olfactory center, the antennal lobe (AL), consists of ∼50 discrete structures called glomeruli that are identifiable from their shape, relative size, and position (Fig.…”

mentioning

confidence: 99%

Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila

Anzo¹,

Sekine²,

Makihara³

et al. 2017

Genes Dev.

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Proper function of the neural network results from the precise connections between axons and dendrites of presynaptic and postsynaptic neurons, respectively. In the Drosophila olfactory system, the dendrites of projection neurons (PNs) stereotypically target one of ∼50 glomeruli in the antennal lobe (AL), the primary olfactory center in the brain, and form synapses with the axons of olfactory receptor neurons (ORNs). Here, we show that Eph and Ephrin, the well-known axon guidance molecules, instruct the dendrodendritic segregation during the discrete olfactory map formation. The Eph receptor tyrosine kinase is highly expressed and localized in the glomeruli related to reproductive behavior in the developing AL. In one of the pheromone-sensing glomeruli (DA1), the Eph cell-autonomously regulates its dendrites to reside in a single glomerulus by interacting with Ephrins expressed in adjacent PN dendrites. Our data demonstrate that the trans interaction between dendritic Eph and Ephrin is essential for the PN dendritic boundary formation in the DA1 olfactory circuit, potentially enabling strict segregation of odor detection between pheromones and the other odors.

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Genetic Control of Wiring Specificity in the Fly Olfactory System

Cited by 99 publications

References 66 publications

Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-Cell RNA Sequencing

Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-Cell RNA Sequencing

Construction of functional neuronal circuitry in the olfactory bulb

Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila

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