Genetic control of multiple esterases from needles and macrogametophytes ofPicea abies

Abstract

The patterns of multiple esterases from needles and macrogametophytes ofPicea abies were examined by polyacrylamide-gel disc-electrophoresis. There are three patterns: (1) two slow-migrating bands, (2) three fast-migrating bands, and (3) a combination of (1) and (2). Re-electrophoresis of the individual bands and genetic analysis demonstrated the existence of two distinct isozymes controlled by two alleles at one locus. In a native population the esterase patterns were in a Hardy-Weinberg equilibrium. In the offspring of crosses between clonal grafts which represent individual trees with different esterase patterns, the patterns segregated in Mendelian ratios. In the haploid macrogametophytes of individual trees they also segregated as expected, i.e. in a 1:1 ratio. This latter fact allows a genetic analysis of individual trees without crossing experiments by investigation of the isozyme patterns only.

Levels of genetic variation in European silver fir (Abies alba)

“…With this method, initiated by Bartels (1971) and explained in detail by Feret and Bergmann (1976), it was possible to assign single activity zones in zymograms of most enzyme systems to individual gene loci, since there were no significant deviations from the 1:1 ratio of alternatively occurring isozyme variants expected for simple allelic segregations. Details of this procedure and the resulting data from fir seed samples will be given elsewhere.…”

Genetic diversity in managed subpopulations of Norway spruce [Picea abies (L.) Karst.]

“…Morphological markers (Langner, 1953), isoenzymes (Bartels, 1971;Morgante and Vendramin, 1991;Krutovskii and Bergmann, 1995;Müller-Starck, 1995;Geburek, 1999) or random amplified polymorphic DNAs (RAPDs) (Collignon and Favre, 2000) have been used to assess the population genetic structure and gene dispersal.…”

Inheritance of RAPD fragments in haploid and diploid tissues of Pinus sylvestris (L.)

“…Conifers are particularly well suited for studies on RAPD inheritance because they have both haploid and *Correspondence diploid tissues (Bartels, 1971). So far, however, this advantage has not been fully utilized and only one type of tissue (haploid or diploid) was used in the earlier studies (Carlson et a!., 1991;Tulsieram et a!., 1992;Kaya & Neale, 1993;Nelson et a!., 1993).…”