Genetic control of lymphocyte antigens in sheep: TheOLA complex and two minor loci

Millot, P

doi:10.1007/bf01570447

Cited by 21 publications

(11 citation statements)

References 9 publications

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“…Lymphocyte alloantigen polymorphisms which are not coded by the MHC have been described in cattle (Stear et al, 1982), sheep (Millot, 1979), pigs (Hruban et al, 1978), dogs (Krumbacher & Schnauppauf, 1976), and horses (Lazary et al, 1982). It will be important to keep these non-MHC antigens in mind when conducting disease association studies in domestic species.…”

Section: Discussionmentioning

confidence: 99%

Lymphocyte alloantigens of the horse. III. ELY‐2.1: a lymphocyte alloantigen not coded for by the MHC

Antczak

1984

Animal Blood Groups and Biochemical Genetics

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Summary A new polymorphic locus of the horse which has several unusual properties is described. The suggested name for the locus is ELY‐2. The gene product of one allele at this locus, designated ELY‐2.1, has been identified with antisera raised as a result of pregnancy. Antibody to ELY‐2.1 was first detected on day 55 after conception in the serum of a mare in first pregnancy. This early onset of antibody is similar to that seen for antibody to ELA antigens, and suggests that the source of the antigenic stimulus may be the tissue of the equine endometrial cups. The antisera identifying ELY‐2.1 are cytotoxic and kill all peripheral blood lymphocytes from horses carrying the antigen. ELY‐2.1 is a cell surface molecule expressed on lymphocytes, erythrocytes, and platelets. Other cell types have not been investigated. The overall phenotypic frequency of ELY‐2.1 in several horse breeds was 16 %. The ELY‐2.1 antigen is controlled by an autosomal, dominant gene which is not coded by the ELA region (the major histocompatibility complex of the horse), nor is it identical to the ELY‐1 locus, which codes for another cell surface alloantigen of equine lymphocytes. Stimulator cells carrying ELY‐2.1 did not induce proliferation of ELY‐2.1 negative responder cells in mixed cultures of horse lymphocytes. Attempts to raise alloantisera to other alleles of the ELY‐2 locus through immunization with lymphocytes were unsuccessful. It is possible that the alternate allele(s) does not code for a gene product which is expressed. The function and biochemical nature of the ELY‐2.1 molecule are unknown.

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Section: Discussionmentioning

confidence: 99%

Lymphocyte alloantigens of the horse. III. ELY‐2.1: a lymphocyte alloantigen not coded for by the MHC

Antczak

1984

Animal Blood Groups and Biochemical Genetics

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“…level of allelic variation at different loci varies (Klein 1986). In domestic animal species serological evidence has been presented for two or more polymorphic class I loci in the dog (Vriesendorp et al 1977), the pig (Vaiman et al 1979), and the sheep (Millot 1979). In the horse and cow it has been more difficult to demonstrate multiple expressed class T loci.…”

Section: Discussionmentioning

confidence: 99%

At least two loci encode polymorphic class I MHC antigens in the horse

et al. 1988

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Six monoclonal antibodies and ten alloantisera were used to precipitate cell surface molecules of approximately 44 kDa (class I MHC antigens) from radiolabelled equine peripheral blood lymphocytes. All ten antisera were raised against antigens of a single donor horse (horse 0834, ELA-A2,-A2). Four methods of producing antisera were compared: one or two pregnancies, skin allografting, and skin grafting followed by pregnancy. Immunization by pregnancy appeared to produce antibodies against class I products only, while skin grafting raised antibodies to class I1 antigens as well. Nine of the antisera were raised across an entire MHC haplotype barrier, while one recipient carried the ELA-A2 antigen of the donor. The pregnancy antiserum raised across this barrier probably identifies a second polymorphic class I locus in the horse. Sequential immunoprecipitation using this antiserum in the first stage and an anti-MHC haplotype antiserum or monoclonal antibody reagent in the second stage supported this hypothesis. Gene products of this second ELA class I locus are immunogenic in pregnancy.

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“…vided by Dr Stear and Dr Spooner are listed in Table 4; these sera, which have been previously published (Stear & Spooner, 1982), were used at the dilutions recommended and represent the specificities ED1,2,3/4,5/6,7,8,9,12 and 13. Eighteen antisera were used, each at two dilutions, to define the OLA specificities listed in Table 3; these antisera were produced by immunizing sheep of the Prtalpe breed as described previously (Millot, 1979).…”

Section: Sera Used In the Cytotoxic Testmentioning

confidence: 99%

“…This led, in preliminary studies, to weak or ambiguous antigen definition and it was therefore decided empirically to extend the test by 30 min before the addition of C' and 60 min afterwards. This modified test, which gave antigen definition comparable with that previously obtained in Oxford, is referred to in this paper as the 'long test' and was as follows: 1 pl antiserum + 1 pl cell suspension Animal Blood Groups and Biochemical Genetics 16 (198s) (3x 106 cells/ml) incubated for 60 min at 22 "C, 4 p1 C' added, followed by incubation for 160 min at 22 "C. The test used by Millot, referred to in this paper as the 'short test', was as follows: 2 pl antiserum + 1 pl cell suspension (3x 106 cells/ml) + 1 pl C' incubated for 55 min at 37 "C. Both tests were terminated by the addition of eosin and formalin as described previously (Cullen et al, 1982;Millot, 1979).…”

Section: Equivalent Serum Designationsmentioning

confidence: 99%

“…Since 1970 five groups in Europe have begun independent studies of the sheep major histocompatibility system (MHS). Using the complement-dependent cytotoxicity test and sera from parous or immunized sheep, these groups each identified from 13 to 31 provisional lymphocyte specificities which were apparently controlled by at least two chief genetic loci (Millot, 1971(Millot, , 1979Ford, 1974Ford, , 1975Ford & Elves, 1974;Schmid, Cwik & Forschner, 1975;Stear & Spooner, 1981;Cullen et al, 1982;Cullen, Brownlie & Kimberlin, 1984), and a third locus was recently described (Millot, 1983). Although the studies of Ford & Elves and of Schmid, Cwik & Forschner ended in the mid-l970's, and that of Stear & Spooner in 1980, some exchange of antisera has been possible between the different groups for the cornparison of specificities.…”

Section: Introductionmentioning

confidence: 99%

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Sheep histo compatibility antigens: a population level comparison between lymphocyte antigens previously defined in France, England and Scotland, and sheep red cell groups

Cullen

Millot

Nguyen

1985

Animal Blood Groups and Biochemical Genetics

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A comparison test was performed to look for correlations between the three nomenclature systems for sheep histocompatibility antigens which have been previously described in France, England and Scotland. 187 French sheep from a wide variety of breeds were typed for lymphocyte antigens with antisera which detect the OLA, P and E D series of antigens; they were also tested against. 387 uncharacterized French antisera. Six clusters of sera were found which showed correspondence between antigens of at least two of the three nomenclatures; five of these clusters gave high r values of 0.78-0.94. New antisera from French sheep were found which contributed to the above clusters but few additional clusters were noted. No correlation was found between any of the lymphocyte groups of antisera tested and the sheep red cell antigens which were also tested.

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Genetic control of lymphocyte antigens in sheep: TheOLA complex and two minor loci

Cited by 21 publications

References 9 publications

Lymphocyte alloantigens of the horse. III. ELY‐2.1: a lymphocyte alloantigen not coded for by the MHC

Lymphocyte alloantigens of the horse. III. ELY‐2.1: a lymphocyte alloantigen not coded for by the MHC

At least two loci encode polymorphic class I MHC antigens in the horse

Sheep histo compatibility antigens: a population level comparison between lymphocyte antigens previously defined in France, England and Scotland, and sheep red cell groups

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