Genetic control of infection-related development in Magnaporthe oryzae

Li, Guotian; Zhou, Xiaoying; Xu, Jin‐Rong

doi:10.1016/j.mib.2012.09.004

Cited by 163 publications

(201 citation statements)

References 55 publications

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“…This gene (named FgSCH9 ) encodes a serine/threonine protein kinase that is highly similar to yeast SCH9 . It is well conserved in filamentous fungi and shares limited homology with MEK kinases of the MAP kinase pathways [31]. In this study, we first confirmed the Fgsch9 mutant generated in an earlier study [21] by Southern blot analyses.…”

Section: Resultssupporting

confidence: 64%

The Sch9 Kinase Regulates Conidium Size, Stress Responses, and Pathogenesis in Fusarium graminearum

et al. 2014

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Fusarium head blight caused by Fusarium graminearum is an important disease of wheat and barley worldwide. In a previous study on functional characterization of the F. graminearum kinome, one protein kinase gene important for virulence is orthologous to SCH9 that is functionally related to the cAMP-PKA and TOR pathways in the budding yeast. In this study, we further characterized the functions of FgSCH9 in F. graminearum and its ortholog in Magnaporthe oryzae. The ΔFgsch9 mutant was slightly reduced in growth rate but significantly reduced in conidiation, DON production, and virulence on wheat heads and corn silks. It had increased tolerance to elevated temperatures but became hypersensitive to oxidative, hyperosmotic, cell wall, and membrane stresses. The ΔFgsch9 deletion also had conidium morphology defects and produced smaller conidia. These results suggest that FgSCH9 is important for stress responses, DON production, conidiogenesis, and pathogenesis in F. graminearum. In the rice blast fungus Magnaporthe oryzae, the ΔMosch9 mutant also was defective in conidiogenesis and pathogenesis. Interestingly, it also produced smaller conidia and appressoria. Taken together, our data indicate that the SCH9 kinase gene may have a conserved role in regulating conidium size and plant infection in phytopathogenic ascomycetes.

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Section: Resultssupporting

confidence: 64%

The Sch9 Kinase Regulates Conidium Size, Stress Responses, and Pathogenesis in Fusarium graminearum

et al. 2014

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“…Studies have shown that there is an interaction between Pmk1 MAPK pathway and cAMP signaling (Li et al, 2012) and it is likely that there is cross talk between Mps1 MAPK and Ca 2+ /calcineurin pathways, similar to that found in yeast (Li et al, 2012). One current line of research is to establish the nature of these interactions and to elucidate which processes they affect.…”

Section: Magnaporthe Oryzaementioning

confidence: 66%

Fungal model systems and the elucidation of pathogenicity determinants

Pérez‐Nadales

Nogueira

Baldin

et al. 2014

Fungal Genetics and Biology

144

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“…WscB and other so-called cell surface sensors have been extensively characterized in yeast, where they monitor cell stress, including internal turgor pressure, through the CWI pathway (52)(53)(54). Interestingly, the CWI pathway is essential for appressorium penetration and invasive growth in Magnaporthe oryzae (55). In addition, four WSC proteins in M. haptotylum (H072_10165, H072_2046, H072_6644, and H072_8090) displayed sequence similarity (Յ1eϪ124) to a legume lectin beta-domain-containing protein (H1UW68) identified in the plant-pathogenic fungus Colletotrichum higginsianum.…”

Section: Cell Surface Proteins (I) Proteins Containing the Wsc Domaimentioning

confidence: 99%

Proteome of the Nematode-Trapping Cells of the Fungus Monacrosporium haptotylum

Andersson

Meerupati

Levander

et al. 2013

Appl Environ Microbiol

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Many nematophagous fungi use morphological structures called traps to capture nematodes by adhesion or mechanically. To better understand the cellular functions of adhesive traps, the trap cell proteome of the fungus Monacrosporium haptotylum was characterized. The trap of M. haptotylum consists of a unicellular structure called a knob that develops at the apex of a hypha. Proteins extracted from knobs and mycelia were analyzed using SDS-PAGE and liquid chromatography-tandem mass spectrometry (LC-MS-MS). The peptide sequences were matched against predicted gene models from the recently sequenced M. haptotylum genome. In total, 336 proteins were identified, with 54 expressed at significantly higher levels in the knobs than in the mycelia. The upregulated knob proteins included peptidases, small secreted proteins with unknown functions, and putative cell surface adhesins containing carbohydrate-binding domains, including the WSC domain. Phylogenetic analysis showed that all upregulated WSC domain proteins belonged to a large, expanded cluster of paralogs in M. haptotylum. Several peptidases and homologs of experimentally verified proteins in other pathogenic fungi were also upregulated in the knob proteome. Complementary profiling of gene expression at the transcriptome level showed poor correlation between the upregulation of knob proteins and their corresponding transcripts. We propose that the traps of M. haptotylum contain many of the proteins needed in the early stages of infection and that the trap cells can tightly control the translation and degradation of these proteins to minimize the cost of protein synthesis. N ematode-trapping fungi have the unique ability to capture and infect free-living nematodes (1). Given their potential use as biological control agents for plant-and animal-parasitic nematodes (2), there is much interest in studying their infection biology. To enter the parasitic stage, nematode-trapping fungi develop a unique morphological structure called traps. These traps develop from hyphal branches, either spontaneously or in response to signals from the environment, such as peptides or other compounds released by the host nematode (3). Molecular phylogeny studies have shown that the majority of nematode-trapping fungi belong to a monophyletic group in the order Orbiliales (Ascomycota). Within this clade, the trapping mechanisms have evolved along two major lineages, one leading to species with constricting rings and the other to species with adhesive traps, including three-dimensional networks, knobs, and branches (4-6).Despite large variation in their morphology, adhesive traps share a unique ultrastructure that clearly separates them from vegetative hyphae (3). One feature that is common to all traps is the presence of numerous cytosolic organelles called dense bodies. These organelles have catalase and D-amino acid oxidase activities, which indicates that they are peroxisome-like organelles (3). However, the function of these organelles is not yet fully understood. Another feature of th...

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Genetic control of infection-related development in Magnaporthe oryzae

Cited by 163 publications

References 55 publications

The Sch9 Kinase Regulates Conidium Size, Stress Responses, and Pathogenesis in Fusarium graminearum

The Sch9 Kinase Regulates Conidium Size, Stress Responses, and Pathogenesis in Fusarium graminearum

Fungal model systems and the elucidation of pathogenicity determinants

Proteome of the Nematode-Trapping Cells of the Fungus Monacrosporium haptotylum

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